2.7.2.7 additional information metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone -, 693994 2.7.2.7 additional information pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type 679539 2.7.2.7 additional information semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico. 691411 2.7.2.7 R150F the mutant shows no butyrate kinase II activity 703499 2.7.2.7 R150K the mutant shows 21% of wild type butyrate kinase II activity 703499 2.7.2.7 R215F E332D the mutant shows 0.38% of wild type butyrate kinase II activity 703499 2.7.2.7 R215K the mutant shows 0.44% of wild type butyrate kinase II activity 703499