3.7.1.15	(+)-caryolan-1-ol synthase	transferred. Now EC 4.2.1.138	
3.5.2.B2	(+)-gamma-lactamase	preliminary BRENDA-supplied EC number	
3.1.7.12	(+)-kolavelool synthase	-	
3.5.2.B3	(-)-gamma-lactamase	preliminary BRENDA-supplied EC number	
3.1.7.10	(13E)-labda-7,13-dien-15-ol synthase	-	
3.1.2.30	(3S)-malyl-CoA thioesterase	-	
3.1.1.117	(4-O-methyl)-D-glucuronate---lignin esterase	-	
3.2.1.187	(Ara-f)3-Hyp beta-L-arabinobiosidase	-	
3.2.1.B48	(Ara-f)4-Hyp alpha-L-arabinofuranosidase	preliminary BRENDA-supplied EC number	
3.6.1.65	(d)CTP diphosphatase	-	
3.5.1.134	(indol-3-yl)acetyl-L-aspartate hydrolase	-	
3.8.1.9	(R)-2-haloacid dehalogenase	-	
3.5.1.100	(R)-amidase	-	
3.13.2.3	(R)-S-adenosyl-L-methionine hydrolase (adenosine-forming)	-	
3.1.6.19	(R)-specific secondary-alkylsulfatase (type III)	-	
3.8.1.2	(S)-2-haloacid dehalogenase	-	
3.1.2.17	(S)-methylmalonyl-CoA hydrolase	-	
3.5.1.85	(S)-N-acetyl-1-phenylethylamine hydrolase	-	
3.5.3.26	(S)-ureidoglycine aminohydrolase	-	
3.2.1.63	1,2-alpha-L-fucosidase	-	
3.1.4.36	1,2-cyclic-inositol-phosphate phosphodiesterase	transferred to EC 3.1.4.43	
3.2.1.204	1,3-alpha-isomaltosidase	-	
3.2.1.111	1,3-alpha-L-fucosidase	-	
3.1.2.28	1,4-dihydroxy-2-naphthoyl-CoA hydrolase	-	
3.1.1.25	1,4-lactonase	-	
3.2.1.163	1,6-alpha-D-mannosidase	-	
3.2.1.127	1,6-alpha-L-fucosidase	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	-	
3.5.99.7	1-aminocyclopropane-1-carboxylate deaminase	-	
3.5.1.131	1-carboxybiuret hydrolase	-	
3.2.2.13	1-methyladenosine nucleosidase	-	
3.1.4.10	1-phosphatidylinositol phosphodiesterase	transferred to EC 4.6.1.13	
3.5.4.22	1-pyrroline-4-hydroxy-2-carboxylate deaminase	-	
3.1.1.63	11-cis-retinyl-palmitate hydrolase	-	
3.5.1.113	2'''-acetyl-6'''-hydroxyneomycin C deacetylase	-	
3.1.4.16	2',3'-cyclic-nucleotide 2'-phosphodiesterase	-	
3.1.4.37	2',3'-cyclic-nucleotide 3'-phosphodiesterase	-	
3.13.1.3	2'-hydroxybiphenyl-2-sulfinate desulfinase	-	
3.5.1.112	2'-N-acetylparomamine deacetylase	-	
3.1.3.80	2,3-bisphosphoglycerate 3-phosphatase	-	
3.7.1.24	2,4-diacetylphloroglucinol hydrolase	-	
3.7.1.26	2,4-didehydro-3-deoxy-L-rhamnonate hydrolase	-	
3.3.2.14	2,4-dinitroanisole O-demethylase	-	
3.5.2.13	2,5-dioxopiperazine hydrolase	-	
3.2.1.64	2,6-beta-fructan 6-levanbiohydrolase	-	
3.7.1.19	2,6-dihydroxypseudooxynicotine hydrolase	-	
3.7.1.8	2,6-dioxo-6-phenylhexa-3-enoate hydrolase	-	
3.1.2.24	2-(2-hydroxyphenyl)benzenesulfinate hydrolase	transferred to EC 3.13.1.3	
3.5.1.29	2-(acetamidomethylene)succinate hydrolase	-	
3.5.1.66	2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase	-	
3.3.2.B1	2-amino-2-deoxyisochorismate hydrolase	deleted, replaced by EC 3.3.2.15	
3.5.1.102	2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase	-	
3.1.2.32	2-aminobenzoylacetyl-CoA thioesterase	-	
3.5.99.5	2-aminomuconate deaminase	-	
3.5.1.120	2-aminomuconate deaminase (2-hydroxymuconate-forming)	transferred to EC 3.5.99.11	
3.5.99.11	2-aminomuconate deaminase (2-hydroxymuconate-forming)	-	
3.1.3.63	2-carboxy-D-arabinitol-1-phosphatase	-	
3.1.3.68	2-deoxyglucose-6-phosphatase	-	
3.2.1.112	2-deoxyglucosidase	-	
3.8.1.10	2-haloacid dehalogenase (configuration-inverting)	-	
3.8.1.11	2-haloacid dehalogenase (configuration-retaining)	-	
3.8.1.B1	2-haloacrylate hydratase	preliminary BRENDA-supplied EC number	
3.1.3.87	2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase	-	
3.7.1.13	2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase	-	
3.7.1.25	2-hydroxy-6-oxohepta-2,4-dienoate hydrolase	-	
3.7.1.14	2-hydroxy-6-oxonona-2,4-dienedioate hydrolase	-	
3.6.1.56	2-hydroxy-dATP diphosphatase	-	
3.7.1.9	2-hydroxymuconate-6-semialdehyde hydrolase	-	
3.5.99.10	2-iminobutanoate/2-iminopropanoate deaminase	-	
3.1.3.106	2-lysophosphatidate phosphatase	-	
3.5.99.9	2-nitroimidazole nitrohydrolase	-	
3.1.1.91	2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase	-	
3.5.1.111	2-oxoglutaramate amidase	-	
3.1.3.71	2-phosphosulfolactate phosphatase	-	
3.1.1.57	2-pyrone-4,6-dicarboxylate lactonase	-	
3.2.1.195	20-O-multi-glycoside ginsenosidase	-	
3.1.3.7	3'(2'),5'-bisphosphate nucleotidase	-	
3.1.4.53	3',5'-cyclic-AMP phosphodiesterase	-	
3.1.4.35	3',5'-cyclic-GMP phosphodiesterase	-	
3.1.4.17	3',5'-cyclic-nucleotide phosphodiesterase	-	
3.1.3.97	3',5'-nucleoside bisphosphate phosphatase	-	
3.1.3.6	3'-nucleotidase	-	
3.1.3.30	3'-phosphoadenylylsulfate 3'-phosphatase	deleted, included in EC 3.1.3.31	
3.1.2.B5	3,4-hydroxyphenylacetyl-CoA thioesterase	preliminary BRENDA-supplied EC number	
3.2.1.124	3-deoxy-2-octulosonidase	-	
3.1.3.103	3-deoxy-D-glycero-D-galacto-nonulopyranosonate 9-phosphatase	-	
3.1.3.45	3-deoxy-manno-octulosonate-8-phosphatase	-	
3.2.1.144	3-deoxyoctulosonase	-	
3.7.1.20	3-fumarylpyruvate hydrolase	-	
3.1.2.4	3-hydroxyisobutyryl-CoA hydrolase	-	
3.1.1.105	3-O-acetylpapaveroxine carboxylesterase	-	
3.1.1.24	3-oxoadipate enol-lactonase	-	
3.7.1.28	3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase	-	
3.1.3.38	3-phosphoglycerate phosphatase	-	
3.1.3.8	3-phytase	-	
3.13.1.4	3-sulfinopropanoyl-CoA desulfinase	-	
3.2.1.105	3alpha(S)-strictosidine beta-glucosidase	-	
3.7.1.22	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (ring-opening)	-	
3.7.1.17	4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase	-	
3.5.1.63	4-acetamidobutyrate deacetylase	-	
3.5.1.51	4-acetamidobutyryl-CoA deacetylase	-	
3.2.1.141	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase	-	
3.5.1.B18	4-aminobenzoylglutamate hydrolase	preliminary BRENDA-supplied EC number	
3.1.1.16	4-carboxymethyl-4-hydroxyisocrotonolactonase	deleted, reaction is that of EC 5.3.3.4 and EC 3.1.1.24	
3.8.1.6	4-chlorobenzoate dehalogenase	-	
3.8.1.7	4-chlorobenzoyl-CoA dehalogenase	-	
3.5.1.B13	4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase	preliminary BRENDA-supplied EC number	
3.2.1.182	4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase	-	
3.1.2.23	4-hydroxybenzoyl-CoA thioesterase	-	
3.5.1.37	4-L-aspartylglycosylamine amidohydrolase	deleted, identical to EC 3.5.1.26	
3.5.1.67	4-methyleneglutaminase	-	
3.1.1.44	4-methyloxaloacetate esterase	-	
3.1.3.41	4-nitrophenylphosphatase	-	
3.1.3.26	4-phytase	-	
3.1.1.27	4-pyridoxolactonase	-	
3.1.1.92	4-sulfomuconolactone hydrolase	-	
3.1.12.1	5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming)	-	
3.1.3.88	5''-phosphoribostamycin phosphatase	-	
3.6.1.59	5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase	-	
3.6.1.62	5'-(N7-methylguanosine 5'-triphospho)-[mRNA] hydrolase	-	
3.1.13.B1	5'-3' exoribonuclease	preliminary BRENDA-supplied EC number	
3.6.1.20	5'-acylphosphoadenosine hydrolase	-	
3.5.4.41	5'-deoxyadenosine deaminase	-	
3.1.3.89	5'-deoxynucleotidase	-	
3.1.3.5	5'-nucleotidase	-	
3.1.1.66	5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase	-	
3.1.3.104	5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase	-	
3.5.1.30	5-aminopentanamidase	-	
3.13.1.2	5-deoxyribos-5-ylhomocysteinase	deleted, activity most probably due to EC 4.4.1.21	
3.5.99.8	5-nitroanthranilic acid aminohydrolase	-	
3.5.2.9	5-oxoprolinase (ATP-hydrolysing)	-	
3.1.1.104	5-phospho-D-xylono-1,4-lactonase	-	
3.1.3.72	5-phytase	-	
3.1.1.33	6-acetylglucose deacetylase	-	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	-	
3.5.1.117	6-aminohexanoate-oligomer endohydrolase	-	
3.5.1.46	6-aminohexanoate-oligomer exohydrolase	-	
3.1.1.99	6-deoxy-6-sulfogluconolactonase	-	
3.7.1.18	6-oxocamphor hydrolase	-	
3.7.1.21	6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase	-	
3.2.1.85	6-phospho-beta-galactosidase	-	
3.2.1.86	6-phospho-beta-glucosidase	-	
3.1.1.31	6-phosphogluconolactonase	-	
3.1.4.56	7,8-dihydroneopterin 2',3'-cyclic phosphate phosphodiesterase	-	
3.1.3.91	7-methylguanosine nucleotidase	-	
3.6.1.69	8-oxo-(d)GTP phosphatase	-	
3.6.1.58	8-oxo-dGDP phosphatase	-	
3.6.1.55	8-oxo-dGTP diphosphatase	-	
3.5.4.32	8-oxoguanine deaminase	-	
3.2.2.B5	8-oxoguanine DNA glycosylase	preliminary BRENDA-supplied EC number	
3.1.2.11	acetoacetyl-CoA hydrolase	-	
3.1.1.54	acetoxybutynylbithiophene deacetylase	-	
3.1.2.1	acetyl-CoA hydrolase	-	
3.1.1.80	acetylajmaline esterase	-	
3.1.1.71	acetylalkylglycerol acetylhydrolase	-	
3.1.1.7	acetylcholinesterase	-	
3.5.1.B4	acetylcitrulline deacetylase	preliminary BRENDA-supplied EC number	
3.1.1.6	acetylesterase	-	
3.5.1.34	acetylhistidine deacetylase	deleted, identical to EC 3.4.13.5	
3.5.1.16	acetylornithine deacetylase	-	
3.5.1.62	acetylputrescine deacetylase	-	
3.7.1.6	acetylpyruvate hydrolase	-	
3.1.1.55	acetylsalicylate deacetylase	-	
3.5.1.48	acetylspermidine deacetylase	-	
3.1.1.72	acetylxylan esterase	-	
3.4.24.8	Achromobacter iophagus collagenase	transferred to EC 3.4.24.3	
3.1.3.2	acid phosphatase	-	
3.1.3.77	acireductone synthase	-	
3.1.1.95	aclacinomycin methylesterase	-	
3.4.99.1	acrocylindricum proteinase	transferred to EC 3.4.23.28	
3.4.23.28	Acrocylindropepsin	-	
3.4.21.10	acrosin	-	
3.4.22.14	actinidain	-	
3.1.1.39	actinomycin lactonase	-	
3.5.1.70	aculeacin-A deacylase	-	
3.1.2.20	acyl-CoA hydrolase	-	
3.5.1.97	acyl-homoserine-lactone acylase	-	
3.5.1.17	acyl-lysine deacylase	-	
3.5.1.40	acylagmatine amidase	-	
3.4.14.3	acylamino-acid-releasing enzyme	transferred to EC 3.4.19.1	
3.4.19.1	acylaminoacyl-peptidase	-	
3.1.1.28	acylcarnitine hydrolase	-	
3.1.1.23	acylglycerol lipase	-	
3.4.19.10	acylmuramoyl-Ala peptidase	transferred to EC 3.5.1.28	
3.4.17.7	acylmuramoyl-alanine carboxypeptidase	transferred to EC 3.4.19.10	
3.1.1.77	acyloxyacyl hydrolase	-	
3.6.1.7	acylphosphatase	-	
3.7.1.5	acylpyruvate hydrolase	-	
3.4.24.B40	ADAM 13 enpopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.86	ADAM 17 endopeptidase	-	
3.4.24.B8	ADAM1 endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.81	ADAM10 endopeptidase	-	
3.4.24.B10	ADAM12 endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.B28	ADAM15	preliminary BRENDA-supplied EC number	
3.4.24.B27	ADAM19	preliminary BRENDA-supplied EC number	
3.4.24.B9	ADAM9 endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.46	adamalysin	-	
3.4.24.82	ADAMTS-4 endopeptidase	-	
3.4.24.B11	ADAMTS1 endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.87	ADAMTS13 endopeptidase	-	
3.4.24.B12	ADAMTS5 endopeptidase	preliminary BRENDA-supplied EC number	
3.4.22.39	adenain	-	
3.5.4.2	adenine deaminase	-	
3.2.2.31	adenine glycosylase	-	
3.5.4.4	adenosine deaminase	-	
3.2.2.7	adenosine nucleosidase	-	
3.1.11.7	adenosine-5'-diphospho-5'-[DNA] diphosphatase	transferred to EC 3.6.1.71	
3.6.1.71	adenosine-5'-diphospho-5'-[DNA] diphosphatase	-	
3.5.4.17	adenosine-phosphate deaminase	-	
3.6.1.14	adenosine-tetraphosphatase	-	
3.6.1.3	adenosinetriphosphatase	deleted, enzymes previously listed under this number are now listed separately under EC 5.6 and EC 7	
3.6.1.4	adenosinetriphosphatase (Mg-activated)	deleted, included in EC 3.6.1.3	
3.1.3.73	adenosylcobalamin/alpha-ribazole phosphatase	-	
3.5.1.90	adenosylcobinamide hydrolase	-	
3.3.1.1	adenosylhomocysteinase	transferred, now EC 3.13.2.1	
3.13.2.1	adenosylhomocysteinase	-	
3.2.2.9	adenosylhomocysteine nucleosidase	-	
3.1.4.15	adenylyl-[glutamate-ammonia ligase] hydrolase	transferred to EC 2.7.7.89	
3.6.2.1	adenylylsulfatase	-	
3.5.4.7	ADP deaminase	-	
3.1.2.19	ADP-dependent medium-chain-acyl-CoA hydrolase	-	
3.1.2.18	ADP-dependent short-chain-acyl-CoA hydrolase	-	
3.1.3.28	ADP-phosphoglycerate phosphatase	-	
3.1.3.84	ADP-ribose 1''-phosphate phosphatase	-	
3.6.1.13	ADP-ribose diphosphatase	-	
3.2.2.6	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase	-	
3.2.2.24	ADP-ribosyl-[dinitrogen reductase] hydrolase	-	
3.6.1.21	ADP-sugar diphosphatase	-	
3.4.24.B30	aeropyrolysin	preliminary BRENDA-supplied EC number	
3.6.3.53	Ag+-exporting ATPase	transferred to EC 7.2.2.15	
3.4.99.2	agavain	deleted	
3.4.21.28	Agkistrodon serine proteinase	transferred to EC 3.4.21.74	
3.5.3.11	agmatinase	-	
3.5.3.12	agmatine deiminase	-	
3.4.17.6	alanine carboxypeptidase	-	
3.1.1.18	aldonolactonase	deleted, included in EC 3.1.1.17	
3.2.1.16	alginase	deleted	
3.5.5.7	Aliphatic nitrilase	-	
3.1.3.1	alkaline phosphatase	-	
3.3.2.5	alkenylglycerophosphoethanolamine hydrolase	transferred. Now included in EC 3.3.2.2	
3.1.3.59	alkylacetylglycerophosphatase	-	
3.5.1.39	alkylamidase	-	
3.1.4.39	alkylglycerophosphoethanolamine phosphodiesterase	-	
3.8.1.1	alkylhalidase	deleted, now covered by EC 3.8.1.5, haloalkane dehalogenase	
3.1.1.90	all-trans-retinyl ester 13-cis isomerohydrolase	-	
3.5.3.9	allantoate deiminase	-	
3.5.3.4	allantoicase	-	
3.5.2.5	allantoinase	-	
3.4.22.B76	Allium alpha-fibrinogenase AFTEII	preliminary BRENDA-supplied EC number	
3.5.1.54	allophanate hydrolase	-	
3.2.1.93	alpha,alpha-phosphotrehalase	-	
3.2.1.28	alpha,alpha-trehalase	-	
3.2.1.27	alpha-1,3-glucosidase	deleted	
3.2.1.158	alpha-agarase	-	
3.1.1.43	alpha-amino-acid esterase	-	
3.2.1.1	alpha-amylase	-	
3.6.1.63	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-	
3.2.1.177	alpha-D-xyloside xylohydrolase	-	
3.6.3.48	alpha-factor-transporting ATPase	transferred to EC 7.4.2.7	
3.2.1.22	alpha-galactosidase	-	
3.2.1.20	alpha-glucosidase	-	
3.2.1.139	alpha-glucuronidase	-	
3.2.1.79	alpha-L-arabinofuranoside hydrolase	deleted, included in EC 3.2.1.55	
3.2.1.51	alpha-L-fucosidase	-	
3.2.1.40	alpha-L-rhamnosidase	-	
3.4.21.12	alpha-lytic endopeptidase	-	
3.2.1.198	alpha-mannan endo-1,2-alpha-mannanase	-	
3.2.1.24	alpha-mannosidase	-	
3.2.1.49	alpha-N-acetylgalactosaminidase	-	
3.2.1.50	alpha-N-acetylglucosaminidase	-	
3.2.1.159	alpha-neoagaro-oligosaccharide hydrolase	-	
3.4.21.16	Alternaria serine proteinase	deleted	
3.4.21.47	alternative-complement-pathway C3/C5 convertase	-	
3.1.3.107	amicoumacin phosphatase	-	
3.5.1.4	amidase	-	
3.5.3.14	amidinoaspartase	-	
3.4.3.3	aminoacyl-histidine dipeptidase, carnosinase	transferred to EC 3.4.13.3	
3.4.3.4	aminoacyl-methylhistidine dipeptidase, anserinase	transferred to EC 3.4.13.5	
3.1.1.29	aminoacyl-tRNA hydrolase	-	
3.5.4.40	aminodeoxyfutalosine deaminase	-	
3.2.2.30	aminodeoxyfutalosine nucleosidase	-	
3.5.4.8	aminoimidazolase	-	
3.4.1.2	aminopeptidase	transferred to EC 3.4.11.2	
3.4.11.11	aminopeptidase	deleted	
3.4.11.6	aminopeptidase B	-	
3.4.11.20	aminopeptidase Ey	-	
3.4.11.22	aminopeptidase I	-	
3.4.11.24	aminopeptidase S	-	
3.4.11.B8	aminopeptidase TET1	preliminary BRENDA-supplied EC number	
3.4.11.15	aminopeptidase Y	-	
3.6.3.13	aminophospholipid-transporting ATPase	deleted, identical to EC 3.6.3.1, phospholipid-translocating ATPase	
3.5.99.2	aminopyrimidine aminohydrolase	-	
3.4.1.3	aminotripeptidase	transferred to EC 3.4.11.4	
3.4.24.B38	ammodytagin	preliminary BRENDA-supplied EC number	
3.5.4.6	AMP deaminase	-	
3.2.2.4	AMP nucleosidase	-	
3.2.1.117	amygdalin beta-glucosidase	-	
3.2.1.33	amylo-alpha-1,6-glucosidase	-	
3.2.1.69	amylopectin 6-glucanohydrolase	deleted, included in EC 3.2.1.41	
3.2.1.9	amylopectin-1,6-glucosidase	deleted	
3.4.22.31	Ananain	-	
3.4.17.23	angiotensin-converting enzyme 2	-	
3.4.99.3	angiotensinase	deleted	
3.2.1.138	anhydrosialidase	transferred to EC 4.2.2.15	
3.4.24.83	anthrax lethal factor endopeptidase	-	
3.1.1.107	apo-salmochelin esterase	-	
3.6.1.5	apyrase	-	
3.4.21.111	aqualysin 1	-	
3.2.1.99	arabinan endo-1,5-alpha-L-arabinanase	-	
3.2.1.90	arabinogalactan endo-1,3-beta-galactosidase	deleted, not sufficiently characterized	
3.2.1.89	arabinogalactan endo-beta-1,4-galactanase	-	
3.2.1.215	arabinogalactan exo alpha-(1,3)-alpha-D-galactosyl-(1->3)-L-arabinofuranosidase (non-reducing end)	-	
3.6.4.B6	archaeal flagellar ATPase	preliminary BRENDA-supplied EC number	
3.1.3.B14	archaetidylinositol phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.5.3.1	arginase	-	
3.5.3.6	arginine deiminase	-	
3.4.11.B7	arginyl aminopeptidase PH1704	preliminary BRENDA-supplied EC number	
3.4.99.32	Armillaria mellea neutral proteinase	transferred to EC 3.4.24.20	
3.6.3.16	arsenite-transporting ATPase	transferred to EC 7.3.2.7	
3.4.21.17	Arthrobacter serine proteinase	deleted	
3.5.1.13	aryl-acylamidase	-	
3.5.5.5	Arylacetonitrilase	-	
3.5.1.76	Arylalkyl acylamidase	-	
3.1.8.1	aryldialkylphosphatase	-	
3.1.1.2	arylesterase	-	
3.5.1.9	arylformamidase	-	
3.1.6.1	arylsulfatase (type I)	-	
3.4.22.7	asclepain	-	
3.5.1.1	asparaginase	-	
3.4.17.5	aspartate carboxypeptidase	deleted	
3.5.1.15	aspartoacylase	-	
3.4.11.21	aspartyl aminopeptidase	-	
3.4.99.4	aspartylendopeptidase	transferred to EC 3.4.23.12	
3.4.13.16	aspartylphenylalanine dipeptidase	deleted	
3.4.23.18	Aspergillopepsin I	-	
3.4.23.19	Aspergillopepsin II	-	
3.4.4.17	aspergillopeptidase A	transferred to EC 3.4.23.20, EC 3.4.23.21, EC 3.4.23.22, EC 3.4.23.23, EC 3.4.23.24, EC 3.4.23.25, EC 3.4.23.26, EC 3.4.21.103, EC 3.4.23.28, EC 3.4.23.29, and EC 3.4.23.30
-30	
3.4.21.15	Aspergillus alkaline proteinase	transferred to EC 3.4.21.63	
3.1.22.2	Aspergillus deoxyribonuclease K1	-	
3.1.30.1	Aspergillus nuclease S1	-	
3.1.4.8	Aspergillus oryzae ribonuclease	transferred to EC 3.1.27.3	
3.4.21.97	assemblin	-	
3.4.24.21	astacin	-	
3.5.4.18	ATP deaminase	-	
3.6.1.8	ATP diphosphatase	-	
3.6.1.B17	ATP-independent RNA helicase	preliminary BRENDA-supplied EC number	
3.8.1.8	atrazine chlorohydrolase	-	
3.4.24.1	atrolysin A	-	
3.4.24.41	atrolysin B	-	
3.4.24.42	atrolysin C	-	
3.4.24.44	atrolysin E	-	
3.4.24.45	atrolysin F	-	
3.4.24.43	atroxase	-	
3.4.24.29	aureolysin	-	
3.2.1.188	avenacosidase	-	
3.4.24.28	bacillolysin	-	
3.1.27.2	Bacillus subtilis ribonuclease	transferred to EC 4.6.1.22	
3.4.11.10	bacterial leucyl aminopeptidase	-	
3.2.1.167	baicalin-beta-D-glucuronidase	-	
3.5.2.1	barbiturase	-	
3.4.23.35	Barrierpepsin	-	
3.1.1.9	benzoylcholinesterase	deleted	
3.2.1.197	beta-1,2-mannosidase	-	
3.2.1.81	beta-agarase	-	
3.4.13.20	beta-Ala-His dipeptidase	-	
3.2.1.2	beta-amylase	-	
3.2.1.161	beta-apiosyl-beta-glucosidase	-	
3.2.2.11	beta-aspartyl-N-acetylglucosaminidase	-	
3.4.19.5	beta-aspartyl-peptidase	-	
3.4.13.10	beta-aspartyldipeptidase	transferred to EC 3.4.19.5	
3.2.1.30	beta-D-acetylglucosaminidase	deleted, included in EC 3.2.1.52	
3.2.1.38	beta-D-fucosidase	-	
3.2.1.175	beta-D-glucopyranosyl abscisate beta-glucosidase	-	
3.7.1.7	beta-diketone hydrolase	-	
3.2.1.26	beta-fructofuranosidase	-	
3.2.1.146	beta-galactofuranosidase	-	
3.2.1.23	beta-galactosidase	-	
3.6.3.42	beta-glucan-transporting ATPase	transferred to EC 7.5.2.3	
3.2.1.21	beta-glucosidase	-	
3.2.1.31	beta-glucuronidase	-	
3.2.1.43	beta-L-rhamnosidase	-	
3.5.2.6	beta-lactamase	-	
3.4.24.32	beta-Lytic metalloendopeptidase	-	
3.4.99.13	beta-lytic proteinase (Mycobacterium sorangium)	transferred to EC 3.4.24.32	
3.2.1.25	beta-mannosidase	-	
3.2.1.53	beta-N-acetylgalactosaminidase	-	
3.2.1.52	beta-N-acetylhexosaminidase	-	
3.4.11.25	beta-peptidyl aminopeptidase	-	
3.2.1.178	beta-porphyranase	-	
3.2.1.149	beta-primeverosidase	-	
3.2.1.B31	beta-rutinosidase	preliminary BRENDA-supplied EC number	
3.5.1.6	beta-ureidopropionase	-	
3.1.2.26	bile-acid-CoA hydrolase	transferred to EC 2.8.3.25	
3.5.1.12	biotinidase	-	
3.1.1.60	bis(2-ethylhexyl)phthalate esterase	-	
3.6.1.29	bis(5'-adenosyl)-triphosphatase	-	
3.6.1.17	bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)	-	
3.6.1.41	bis(5'-nucleosyl)-tetraphosphatase (symmetrical)	-	
3.1.3.13	bisphosphoglycerate phosphatase	deleted, activity due to EC 5.4.2.11, phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)
	
3.5.1.84	biuret amidohydrolase	-	
3.5.4.23	blasticidin-S deaminase	-	
3.4.22.40	bleomycin hydrolase	-	
3.2.1.102	blood-group-substance endo-1,4-beta-galactosidase	-	
3.4.24.69	bontoxilysin	-	
3.4.24.50	bothrolysin	-	
3.4.24.49	bothropasin	-	
3.4.21.29	Bothrops atrox serine proteinase	transferred to EC 3.4.21.74	
3.4.23.B9	bovine leukemia virus protease	preliminary BRENDA-supplied EC number	
3.4.21.32	brachyurin	-	
3.1.6.22	branched primary-alkylsulfatase	-	
3.2.1.115	branched-dextran exo-1,2-alpha-glucosidase	-	
3.4.22.5	bromelain (juice)	transferred to EC 3.4.22.33	
3.4.22.4	bromelain (stem)	transferred to EC 3.4.22.32, EC  3.4.22.33	
3.5.5.6	bromoxynil nitrilase	-	
3.4.21.102	C-terminal processing peptidase	-	
3.4.21.110	C5a peptidase	-	
3.6.1.38	Ca2+-transporting ATPase	transferred to EC 3.6.3.8	
3.6.3.8	Ca2+-transporting ATPase	transferred to EC 7.2.2.10	
3.6.3.46	cadmium-transporting ATPase	transferred to EC 7.2.2.2	
3.1.3.55	caldesmon-phosphatase	-	
3.4.22.66	calicivirin	-	
3.4.22.17	calpain	transferred to EC 3.4.22.52 and EC 3.4.22.53	
3.4.22.B30	calpain 10	preliminary BRENDA-supplied EC number	
3.4.22.B31	calpain 11	preliminary BRENDA-supplied EC number	
3.4.22.B32	calpain 12	preliminary BRENDA-supplied EC number	
3.4.22.B35	calpain 15	preliminary BRENDA-supplied EC number	
3.4.22.B24	calpain 4	preliminary BRENDA-supplied EC number	
3.4.22.B25	calpain 5	preliminary BRENDA-supplied EC number	
3.4.22.B26	calpain 6	preliminary BRENDA-supplied EC number	
3.4.22.B27	calpain 7	preliminary BRENDA-supplied EC number	
3.4.22.B28	calpain 8	preliminary BRENDA-supplied EC number	
3.4.22.B29	calpain 9	preliminary BRENDA-supplied EC number	
3.4.22.B36	calpain A	preliminary BRENDA-supplied EC number	
3.4.22.B37	calpain B	preliminary BRENDA-supplied EC number	
3.4.22.B38	calpain C	preliminary BRENDA-supplied EC number	
3.4.22.52	calpain-1	-	
3.4.22.53	calpain-2	-	
3.4.22.54	calpain-3	-	
3.5.4.46	cAMP deaminase	-	
3.4.22.26	cancer procoagulant	-	
3.4.23.24	Candidapepsin	-	
3.2.1.87	capsular-polysaccharide endo-1,3-alpha-galactosidase	-	
3.6.3.38	capsular-polysaccharide-transporting ATPase	transferred to EC 7.6.2.12	
3.13.1.5	carbon disulfide hydrolase	-	
3.13.1.7	carbonyl sulfide hydrolase	-	
3.1.1.1	carboxylesterase	-	
3.2.1.203	carboxymethylcellulase	-	
3.1.1.45	carboxymethylenebutenolidase	-	
3.5.2.4	carboxymethylhydantoinase	-	
3.4.2.1	carboxypeptidase A	transferred to EC 3.4.17.1	
3.4.17.1	carboxypeptidase A	-	
3.4.17.15	carboxypeptidase A2	-	
3.4.2.2	carboxypeptidase B	transferred to EC 3.4.17.2	
3.4.17.2	carboxypeptidase B	-	
3.4.16.5	carboxypeptidase C	-	
3.4.16.6	carboxypeptidase D	-	
3.4.17.10	carboxypeptidase E	-	
3.4.17.12	carboxypeptidase M	-	
3.4.17.9	carboxypeptidase S	transferred to EC 3.4.17.4	
3.4.17.18	carboxypeptidase T	-	
3.4.17.19	Carboxypeptidase Taq	-	
3.4.17.20	Carboxypeptidase U	-	
3.4.22.30	Caricain	-	
3.5.1.73	carnitinamidase	-	
3.4.22.36	caspase-1	-	
3.4.22.63	caspase-10	-	
3.4.22.64	caspase-11	-	
3.4.22.B66	caspase-12	preliminary BRENDA-supplied EC number	
3.4.22.55	caspase-2	-	
3.4.22.56	caspase-3	-	
3.4.22.57	caspase-4	-	
3.4.22.58	caspase-5	-	
3.4.22.59	caspase-6	-	
3.4.22.60	caspase-7	-	
3.4.22.61	caspase-8	-	
3.4.22.62	caspase-9	-	
3.4.22.1	cathepsin B	-	
3.4.22.B6	cathepsin B-like protease	preliminary BRENDA-supplied EC number	
3.4.4.9	cathepsin C	transferred to EC 3.4.14.1	
3.4.23.5	cathepsin D	-	
3.4.23.34	cathepsin E	-	
3.4.22.41	cathepsin F	-	
3.4.21.20	cathepsin G	-	
3.4.22.16	cathepsin H	-	
3.4.22.38	cathepsin K	-	
3.4.22.15	cathepsin L	-	
3.4.22.B49	cathepsin L1	preliminary BRENDA-supplied EC number	
3.4.22.B60	cathepsin L2	preliminary BRENDA-supplied EC number	
3.4.22.B62	cathepsin L3	preliminary BRENDA-supplied EC number	
3.4.22.B61	cathepsin L5	preliminary BRENDA-supplied EC number	
3.4.22.42	cathepsin O	-	
3.4.22.B65	cathepsin P	preliminary BRENDA-supplied EC number	
3.4.21.52	cathepsin R	deleted	
3.4.99.33	cathepsin R	deleted	
3.4.22.27	cathepsin S	-	
3.4.22.24	cathepsin T	-	
3.4.22.43	cathepsin V	-	
3.4.18.1	cathepsin X	-	
3.1.21.6	CC-preferring endodeoxyribonuclease	-	
3.6.3.3	Cd2+-exporting ATPase	transferred to EC 7.2.2.21	
3.6.1.26	CDP-diacylglycerol diphosphatase	-	
3.6.1.16	CDP-glycerol diphosphatase	-	
3.1.3.98	CDP-glycerol:N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol glycerophosphotransferase	transferred to EC 3.6.1.68	
3.2.1.4	cellulase	-	
3.2.1.91	cellulose 1,4-beta-cellobiosidase (non-reducing end)	-	
3.2.1.176	cellulose 1,4-beta-cellobiosidase (reducing end)	-	
3.2.1.B42	cellulose 1,4-beta-cellotetraosidase (non-reducing end)	preliminary BRENDA-supplied EC number	
3.1.6.7	cellulose-polysulfatase	-	
3.1.1.41	cephalosporin-C deacetylase	-	
3.5.2.8	cephalosporinase	deleted, included in EC 3.5.2.6	
3.5.1.23	ceramidase	-	
3.1.6.8	cerebroside-sulfatase	-	
3.4.21.48	cerevisin	-	
3.1.1.70	cetraxate benzylesterase	-	
3.6.3.49	channel-conductance-controlling ATPase	transferred to EC 5.6.1.6	
3.6.4.9	chaperonin ATPase	transferred to EC 5.6.1.7	
3.6.4.B10	chaperonin ATPase (protein-folding, protecting from aggregation, protein stabilizing)	preliminary BRENDA-supplied EC number	
3.5.1.74	chenodeoxycholoyltaurine hydrolase	-	
3.5.1.41	chitin deacetylase	-	
3.5.1.105	chitin disaccharide deacetylase	-	
3.2.1.14	chitinase	-	
3.2.1.29	chitobiase	deleted, included in EC 3.2.1.52	
3.2.1.132	chitosanase	-	
3.1.1.42	chlorogenate hydrolase	-	
3.1.1.14	chlorophyllase	-	
3.1.1.100	chlorophyllide a hydrolase	-	
3.6.3.52	chloroplast protein-transporting ATPase	transferred to EC 7.4.2.4	
3.3.2.11	cholesterol-5,6-oxide hydrolase	-	
3.1.6.6	choline-sulfatase	-	
3.1.1.8	cholinesterase	-	
3.1.2.27	choloyl-CoA hydrolase	-	
3.5.1.24	choloylglycine hydrolase	-	
3.1.6.9	chondro-4-sulfatase	-	
3.1.6.10	chondro-6-sulfatase	-	
3.2.1.34	chondroitinase	deleted, included in EC 3.2.1.35	
3.4.24.67	choriolysin H	-	
3.4.24.66	choriolysin L	-	
3.3.2.13	chorismatase	-	
3.6.4.B11	chromosome segregation protein SegA	preliminary BRENDA-supplied EC number	
3.4.21.39	chymase	-	
3.4.4.11	chymopapain	transferred to EC 3.4.22.6	
3.4.22.6	chymopapain	-	
3.4.23.4	chymosin	-	
3.4.4.5	chymotrypsin	transferred to EC 3.4.21.1	
3.4.21.1	chymotrypsin	-	
3.4.4.6	chymotrypsin B	transferred to EC 3.4.21.1	
3.4.21.2	chymotrypsin C	-	
3.1.2.16	citrate-lyase deacetylase	-	
3.5.1.20	citrullinase	-	
3.6.3.11	Cl--transporting ATPase	deleted, the activity was only ever studied in crude extracts, and is an artifact.	
3.6.4.B8	clamp-loader complex	preliminary BRENDA-supplied EC number	
3.4.21.43	classical-complement-pathway C3/C5 convertase	-	
3.4.11.13	Clostridial aminopeptidase	-	
3.4.4.19	clostridiopeptidase A	transferred to EC 3.4.24.3	
3.4.4.20	clostridiopeptidase B	transferred to EC 3.4.22.8	
3.4.99.5	Clostridium histolyticum collagenase 2	transferred to EC 3.4.24.3	
3.4.22.8	clostripain	-	
3.1.4.40	CMP-N-acylneuraminate phosphodiesterase	-	
3.4.21.22	coagulation factor IXa	-	
3.4.21.21	coagulation factor VIIa	-	
3.4.21.6	coagulation factor Xa	-	
3.4.21.27	coagulation factor XIa	-	
3.4.21.38	coagulation factor XIIa	-	
3.7.1.12	cobalt-precorrin 5A hydrolase	-	
3.1.1.84	cocaine esterase	-	
3.4.24.30	coccolysin	-	
3.4.21.44	complement component C5 convertase	transferred to EC 3.4.21.43	
3.4.21.46	complement factor D	-	
3.4.21.45	complement factor I	-	
3.4.21.41	complement subcomponent C1r	-	
3.4.21.42	complement subcomponent C1s	-	
3.2.1.126	coniferin beta-glucosidase	-	
3.4.22.B5	CPB protease	preliminary BRENDA-supplied EC number	
3.4.99.6	crayfish low-molecular-weight proteinase	transferred to EC 3.4.24.21	
3.5.3.3	creatinase	-	
3.5.2.10	creatininase	-	
3.5.4.21	creatinine deaminase	-	
3.1.21.10	crossover junction endodeoxyribonuclease	-	
3.1.22.4	crossover junction endodeoxyribonuclease	transferred to EC 3.1.21.10	
3.4.21.30	Crotalus adamanteus serine proteinase	transferred to EC 3.4.21.74	
3.4.22.51	cruzipain	-	
3.4.21.B49	ctpB peptidase	preliminary BRENDA-supplied EC number	
3.6.3.54	Cu+-exporting ATPase	transferred to EC 7.2.2.8	
3.6.3.4	Cu2+-exporting ATPase	transferred to EC 7.2.2.9	
3.4.21.25	cucumisin	-	
3.1.1.74	cutinase	-	
3.5.5.3	cyanate hydrolase	transferred to EC 4.3.99.1	
3.5.5.4	cyanoalanine nitrilase	-	
3.4.15.6	cyanophycinase	-	
3.5.2.15	cyanuric acid amidohydrolase	-	
3.10.1.2	cyclamate sulfohydrolase	-	
3.1.4.61	cyclic 2,3-diphosphoglycerate hydrolase	-	
3.1.4.59	cyclic-di-AMP phosphodiesterase	-	
3.1.4.52	cyclic-guanylate-specific phosphodiesterase	-	
3.2.1.12	cycloheptaglucanase	deleted, included in EC 3.2.1.54	
3.2.1.13	cyclohexaglucanase	deleted, included in EC 3.2.1.54	
3.7.1.11	cyclohexane-1,2-dione hydrolase	-	
3.7.1.10	cyclohexane-1,3-dione hydrolase	-	
3.2.1.54	cyclomaltodextrinase	-	
3.4.13.6	Cys-Gly dipeptidase	transferred to EC 3.4.11.6	
3.4.3.5	cysteinyl-glycine dipeptidase	transferred to EC 3.4.11.2	
3.4.13.23	cysteinylglycine-S-conjugate dipeptidase	-	
3.4.11.3	cystinyl aminopeptidase	-	
3.5.4.5	cytidine deaminase	-	
3.5.4.1	cytosine deaminase	-	
3.5.4.B9	cytosine deaminase APOBEC3G	preliminary BRENDA-supplied EC number	
3.4.11.14	cytosol alanyl aminopeptidase	-	
3.4.13.18	cytosol nonspecific dipeptidase	-	
3.4.13.22	D-Ala-D-Ala dipeptidase	-	
3.1.1.96	D-aminoacyl-tRNA deacylase	-	
3.1.1.115	D-apionolactonase	-	
3.1.1.30	D-arabinonolactonase	-	
3.5.3.10	D-arginase	-	
3.4.99.B2	D-aspartyl endopeptidase	preliminary BRENDA-supplied EC number	
3.5.1.72	D-benzoylarginine-4-nitroanilide amidase	-	
3.1.3.94	D-galactose 1-phosphate phosphatase	-	
3.5.1.35	D-glutaminase	-	
3.1.3.83	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-	
3.1.3.82	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-	
3.1.6.17	D-lactate-2-sulfatase	-	
3.4.11.19	D-stereospecific aminopeptidase	-	
3.4.24.60	dactylysin	-	
3.5.4.12	dCMP deaminase	-	
3.5.4.13	dCTP deaminase	-	
3.5.4.30	dCTP deaminase (dUMP-forming)	-	
3.6.1.12	dCTP diphosphatase	-	
3.5.1.128	deaminated glutathione amidase	-	
3.4.21.B50	DegQ peptidase	preliminary BRENDA-supplied EC number	
3.5.4.14	deoxycytidine deaminase	transferred. Now EC 3.5.4.5	
3.1.1.46	Deoxylimonate A-ring-lactonase	-	
3.1.3.34	deoxynucleotide 3'-phosphatase	-	
3.2.2.17	deoxyribodipyrimidine endonucleosidase	-	
3.1.4.5	deoxyribonuclease	transferred to EC 3.1.21.1	
3.1.25.1	deoxyribonuclease (pyrimidine dimer)	-	
3.1.21.1	deoxyribonuclease I	-	
3.1.4.6	deoxyribonuclease II	transferred to EC 3.1.22.1	
3.1.22.1	deoxyribonuclease II	-	
3.1.21.2	deoxyribonuclease IV	-	
3.1.21.7	deoxyribonuclease V	-	
3.1.22.3	deoxyribonuclease V	transferred to EC 3.1.21.7	
3.1.22.5	deoxyribonuclease X	-	
3.4.19.15	desampylase	-	
3.4.24.88	desampylase	transferred to EC 3.4.19.15	
3.4.24.39	deuterolysin	-	
3.2.1.95	dextran 1,6-alpha-isomaltotriosidase	-	
3.2.1.11	dextranase	-	
3.1.5.1	dGTPase	-	
3.8.2.1	di-isopropyl-fluorophosphatase	transferred to EC 3.1.8.2	
3.1.3.81	diacylglycerol diphosphate phosphatase	transferred. Now EC 3.6.1.75	
3.6.1.75	diacylglycerol diphosphate phosphatase	-	
3.6.1.60	diadenosine hexaphosphate hydrolase (AMP-forming)	-	
3.6.1.61	diadenosine hexaphosphate hydrolase (ATP-forming)	-	
3.5.4.26	diaminohydroxyphosphoribosylaminopyrimidine deaminase	-	
3.2.1.134	difructose-dianhydride-I hydrolase	transferred. Now EC 4.2.1.179	
3.5.3.20	diguanidinobutanase	-	
3.1.1.35	dihydrocoumarin hydrolase	-	
3.1.2.31	dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase	-	
3.6.1.67	dihydroneopterin triphosphate diphosphatase	-	
3.5.2.3	dihydroorotase	-	
3.5.2.2	dihydropyrimidinase	-	
3.1.8.2	diisopropyl-fluorophosphatase	-	
3.5.3.18	dimethylargininase	-	
3.4.22.20	dinorphin-converting enzyme	deleted	
3.2.1.190	dioscin glycosidase (3-O-beta-D-Glc-diosgenin-forming)	-	
3.2.1.189	dioscin glycosidase (diosgenin-forming)	-	
3.4.13.11	dipeptidase	transferred to EC 3.4.13.18, EC 3.4.13.19	
3.4.13.21	dipeptidase E	-	
3.4.15.3	dipeptidyl carboxypeptidase	transferred to EC 3.4.15.5	
3.4.14.6	dipeptidyl-dipeptidase	-	
3.4.14.1	dipeptidyl-peptidase I	-	
3.4.14.2	dipeptidyl-peptidase II	-	
3.4.14.4	dipeptidyl-peptidase III	-	
3.4.14.5	dipeptidyl-peptidase IV	-	
3.6.1.52	diphosphoinositol-polyphosphate diphosphatase	-	
3.1.6.11	disulfoglucosamine-6-sulfatase	-	
3.1.4.31	DNA 5'-dinucleotidohydrolase	transferred to EC 3.1.11.4	
3.6.4.12	DNA helicase	transferred to EC 5.6.2.3 and EC 5.6.2.4	
3.1.12.2	DNA-3'-diphospho-5'-guanosine diphosphatase	transferred to EC 3.6.1.72	
3.6.1.72	DNA-3'-diphospho-5'-guanosine diphosphatase	-	
3.2.2.20	DNA-3-methyladenine glycosylase I	-	
3.2.2.21	DNA-3-methyladenine glycosylase II	-	
3.2.2.15	DNA-deoxyinosine glycosylase	-	
3.2.2.23	DNA-formamidopyrimidine glycosylase	-	
3.1.99.B4	DNase xthA	preliminary BRENDA-supplied EC number	
3.1.5.B1	dNTPase	preliminary BRENDA-supplied EC number	
3.1.2.21	dodecanoyl-[acyl-carrier-protein] hydrolase	-	
3.1.3.51	dolichyl-phosphatase	-	
3.6.1.43	dolichyldiphosphatase	-	
3.1.4.48	dolichylphosphate-glucose phosphodiesterase	-	
3.1.4.49	dolichylphosphate-mannose phosphodiesterase	-	
3.5.4.37	double-stranded RNA adenine deaminase	-	
3.2.2.28	double-stranded uracil-DNA glycosylase	-	
3.1.7.7	drimenol synthase	transferred to EC 4.2.3.194	
3.4.21.B3	duodenase	preliminary BRENDA-supplied EC number	
3.6.1.23	dUTP diphosphatase	-	
3.6.1.50	dynamin GTPase	transferred to EC 3.6.5.5	
3.6.5.5	dynamin GTPase	-	
3.6.1.33	dynein ATPase	transferred to EC 3.6.4.2	
3.6.4.2	dynein ATPase	transferred to EC 5.6.1.2	
3.4.99.27	Echis carinatus prothrombin-activating proteinase	deleted	
3.5.4.44	ectoine hydrolase	-	
3.4.22.B68	EhCP4 proteinase	preliminary BRENDA-supplied EC number	
3.4.21.11	elastase	transferred to EC 3.4.21.36, EC 3.4.21.37	
3.5.2.18	enamidase	-	
3.2.1.211	endo-(1->3)-fucoidanase	-	
3.2.1.212	endo-(1->4)-fucoidanase	-	
3.2.1.6	endo-1,3(4)-beta-glucanase	-	
3.2.1.32	endo-1,3-beta-xylanase	-	
3.2.1.8	endo-1,4-beta-xylanase	-	
3.2.1.97	endo-alpha-N-acetylgalactosaminidase	-	
3.2.1.129	endo-alpha-sialidase	-	
3.2.1.202	endo-chitodextinase	-	
3.4.22.19	endo-oligopeptidase	transferred to EC 3.4.24.15	
3.2.1.15	endo-polygalacturonase	-	
3.1.4.30	endodeoxyribonuclease	transferred to EC 3.1.21.2	
3.1.25.2	endodeoxyribonuclease (apurinic or apyrimidinic)	transferred to EC 4.2.99.18	
3.1.4.32	endodeoxyribonuclease (ATP- and S-adenosylmethionine-dependent)	deleted	
3.1.4.33	endodeoxyribonuclease (ATP-hydrolysing)	deleted	
3.2.1.109	endogalactosaminidase	-	
3.2.1.123	endoglycosylceramidase	-	
3.1.99.B6	endonuclease Bax1	preliminary BRENDA-supplied EC number	
3.1.21.B1	endonuclease Q	preliminary BRENDA-supplied EC number	
3.1.99.B5	endonuclease SSO2001	preliminary BRENDA-supplied EC number	
3.4.22.B78	endopepidase PH1704	preliminary BRENDA-supplied EC number	
3.4.21.92	Endopeptidase Clp	-	
3.4.21.53	Endopeptidase La	-	
3.4.21.67	Endopeptidase So	-	
3.2.1.210	endoplasmic reticulum Man8GlcNAc2 1,2-alpha-mannosidase	-	
3.2.1.209	endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase	-	
3.6.1.10	endopolyphosphatase	-	
3.1.4.24	endoribonuclease III	deleted	
3.4.24.71	endothelin-converting enzyme 1	-	
3.4.23.10	endothia acid proteinase	transferred to EC 3.4.23.22	
3.4.23.22	Endothiapepsin	-	
3.1.27.6	Enterobacter ribonuclease	transferred to EC 4.6.1.21	
3.4.4.8	enteropeptidase	transferred to EC 3.4.21.9	
3.4.21.9	enteropeptidase	-	
3.4.21.33	Entomophthora collagenolytic proteinase	deleted	
3.4.24.12	envelysin	-	
3.3.2.3	epoxide hydrolase	deleted. Now classified as EC 3.3.2.9, microsomal epoxide hydrolase, and EC 3.3.2.10, soluble epoxide hydrolase	
3.4.21.114	equine arterivirus serine peptidase	-	
3.4.23.B3	equine infectious anemia virus proteinase	preliminary BRENDA-supplied EC number	
3.1.1.B13	ergosteryl-3beta-O-L-aspartate hydrolase	preliminary BRENDA-supplied EC number	
3.1.1.113	ethyl acetate hydrolase	-	
3.4.21.56	euphorbain	deleted	
3.4.99.7	euphorbain	deleted	
3.2.1.214	exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end)	-	
3.2.1.165	exo-1,4-beta-D-glucosaminidase	-	
3.2.1.217	exo-acting protein-alpha-N-acetylgalactosaminidase	-	
3.2.1.18	exo-alpha-sialidase	-	
3.2.1.200	exo-chitinase (non-reducing end)	-	
3.2.1.201	exo-chitinase (reducing end)	-	
3.2.1.B47	exo-fucoidanase	preliminary BRENDA-supplied EC number	
3.2.1.82	exo-poly-alpha-digalacturonosidase	-	
3.1.11.3	exodeoxyribonuclease (lambda-induced)	-	
3.1.11.4	exodeoxyribonuclease (phage SP3-induced)	-	
3.1.4.25	exodeoxyribonuclease I	transferred to EC 3.1.11.1	
3.1.11.1	exodeoxyribonuclease I	-	
3.1.4.26	exodeoxyribonuclease II	deleted	
3.1.4.27	exodeoxyribonuclease II	transferred to EC 3.1.11.2	
3.1.11.2	exodeoxyribonuclease III	-	
3.1.4.28	exodeoxyribonuclease IV	transferred to EC 3.1.11.3	
3.1.11.5	exodeoxyribonuclease V	-	
3.1.11.6	exodeoxyribonuclease VII	-	
3.6.1.11	exopolyphosphatase	-	
3.1.4.20	exoribonuclease	transferred to EC 3.1.13.1	
3.1.13.2	exoribonuclease H	-	
3.1.13.1	exoribonuclease II	-	
3.2.1.B1	extracellular agarase	preliminary BRENDA-supplied EC number	
3.6.1.18	FAD diphosphatase	-	
3.4.22.B69	falcipain-2	preliminary BRENDA-supplied EC number	
3.1.7.6	farnesyl diphosphatase	-	
3.5.1.99	fatty acid amide hydrolase	-	
3.6.3.47	fatty-acyl-CoA-transporting ATPase	transferred to EC 7.6.2.4	
3.1.1.67	fatty-acyl-ethyl-ester synthase	-	
3.6.3.30	Fe3+-transporting ATPase	transferred to EC 7.2.2.7	
3.4.23.B4	Feline immunodeficiency virus protease	preliminary BRENDA-supplied EC number	
3.1.1.73	feruloyl esterase	-	
3.4.21.B28	fibroblast activation protein alpha subunit	preliminary BRENDA-supplied EC number	
3.4.24.72	fibrolase	-	
3.4.22.3	ficain	-	
3.4.4.12	ficin	transferred to EC 3.4.22.3	
3.1.99.B1	flap endonuclease-1	preliminary BRENDA-supplied EC number	
3.4.24.76	flavastacin	-	
3.4.21.91	Flavivirin	-	
3.1.2.29	fluoroacetyl-CoA thioesterase	-	
3.1.3.102	FMN hydrolase	-	
3.4.19.9	folate gamma-glutamyl hydrolase	-	
3.5.1.49	formamidase	-	
3.5.3.5	formimidoylaspartate deiminase	-	
3.5.3.8	formimidoylglutamase	-	
3.5.3.13	formimidoylglutamate deiminase	-	
3.1.2.10	formyl-CoA hydrolase	-	
3.5.1.8	formylaspartate deformylase	-	
3.5.1.31	formylmethionine deformylase	-	
3.5.1.10	formyltetrahydrofolate deformylase	-	
3.4.24.74	fragilysin	-	
3.2.1.153	fructan beta-(2,1)-fructosidase	-	
3.2.1.154	fructan beta-(2,6)-fructosidase	-	
3.2.1.80	fructan beta-fructosidase	-	
3.1.3.46	fructose-2,6-bisphosphate 2-phosphatase	-	
3.1.3.54	fructose-2,6-bisphosphate 6-phosphatase	-	
3.1.3.11	fructose-bisphosphatase	-	
3.4.22.33	Fruit bromelain	-	
3.4.24.B17	FtsH endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.B20	FtsH protease	preliminary BRENDA-supplied EC number	
3.1.6.B2	fucoidan-2-sulfatase	preliminary BRENDA-supplied EC number	
3.2.1.44	fucoidanase	transferred to EC 3.2.1.211 and EC 3.2.1.212	
3.7.1.2	fumarylacetoacetase	-	
3.1.1.87	fumonisin B1 esterase	-	
3.4.21.75	Furin	-	
3.1.1.48	fusarinine-C ornithinesterase	-	
3.2.2.26	futalosine hydrolase	-	
3.2.1.145	galactan 1,3-beta-galactosidase	-	
3.2.1.164	galactan endo-1,6-beta-galactosidase	-	
3.2.1.181	galactan endo-beta-1,3-galactanase	-	
3.2.1.213	galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)	-	
3.1.1.26	galactolipase	-	
3.2.1.46	galactosylceramidase	-	
3.2.1.47	galactosylgalactosylglucosylceramidase	deleted, reaction covered by EC 3.2.1.22	
3.2.1.67	galacturonan 1,4-alpha-galacturonidase	-	
3.4.24.38	gametolysin	-	
3.4.14.13	gamma-D-glutamyl-L-lysine dipeptidyl-peptidase	-	
3.4.19.11	gamma-D-glutamyl-meso-diaminopimelate peptidase	-	
3.5.1.118	gamma-glutamyl hercynylcysteine S-oxide hydrolase	-	
3.4.22.12	gamma-glutamyl hydrolase	transferred to EC 3.4.19.9	
3.5.1.94	gamma-glutamyl-gamma-aminobutyrate hydrolase	-	
3.5.1.123	gamma-glutamylanilide hydrolase	-	
3.4.13.14	gamma-glutamyldipeptidase	deleted	
3.4.21.54	gamma-Renin	-	
3.4.23.3	gastricsin	-	
3.2.1.42	GDP-glucosidase	-	
3.4.24.24	gelatinase A	-	
3.4.24.35	gelatinase B	-	
3.2.1.179	gellan tetrasaccharide unsaturated glucuronosyl hydrolase	-	
3.1.7.11	geranyl diphosphate diphosphatase	-	
3.6.1.68	geranyl diphosphate phosphohydrolase	-	
3.1.7.5	geranylgeranyl diphosphate diphosphatase	-	
3.4.22.47	gingipain K	-	
3.4.22.37	gingipain R	-	
3.2.1.193	ginsenosidase type I	-	
3.2.1.B23	ginsenosidase type II	preliminary BRENDA-supplied EC number	
3.2.1.191	ginsenosidase type III	-	
3.2.1.194	ginsenosidase type IV	-	
3.2.1.192	ginsenoside Rb1 beta-glucosidase	-	
3.4.99.8	Gliocladium proteinase	deleted	
3.4.13.7	Glu-Glu dipeptidase	-	
3.2.1.84	glucan 1,3-alpha-glucosidase	-	
3.2.1.58	glucan 1,3-beta-glucosidase	-	
3.2.1.3	glucan 1,4-alpha-glucosidase	-	
3.2.1.98	glucan 1,4-alpha-maltohexaosidase	-	
3.2.1.133	glucan 1,4-alpha-maltohydrolase	-	
3.2.1.60	glucan 1,4-alpha-maltotetraohydrolase	-	
3.2.1.116	glucan 1,4-alpha-maltotriohydrolase	-	
3.2.1.74	glucan 1,4-beta-glucosidase	-	
3.2.1.70	glucan 1,6-alpha-glucosidase	-	
3.2.1.94	glucan 1,6-alpha-isomaltosidase	-	
3.2.1.71	glucan endo-1,2-beta-glucosidase	-	
3.2.1.59	glucan endo-1,3-alpha-glucosidase	-	
3.2.1.39	glucan endo-1,3-beta-D-glucosidase	-	
3.2.1.75	glucan endo-1,6-beta-glucosidase	-	
3.1.1.17	gluconolactonase	-	
3.5.99.6	glucosamine-6-phosphate deaminase	-	
3.1.3.10	glucose-1-phosphatase	-	
3.1.4.51	glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase	-	
3.1.3.9	glucose-6-phosphatase	-	
3.4.19.16	glucosinolate gamma-glutamyl hydrolase	-	
3.1.3.85	glucosyl-3-phosphoglycerate phosphatase	-	
3.2.1.45	glucosylceramidase	-	
3.2.1.208	glucosylglycerate hydrolase	-	
3.1.3.69	glucosylglycerol 3-phosphatase	-	
3.1.6.18	glucuronate-2-sulfatase	-	
3.2.1.136	glucuronoarabinoxylan endo-1,4-beta-xylanase	-	
3.2.1.56	glucuronosyl-disulfoglucosamine glucuronidase	-	
3.1.1.B11	glucuronoyl esterase	deleted, replaced by EC 3.1.1.117	
3.4.17.11	glutamate carboxypeptidase	-	
3.4.17.21	Glutamate carboxypeptidase II	-	
3.5.1.38	glutamin-(asparagin-)ase	-	
3.5.1.2	glutaminase	-	
3.4.11.7	glutamyl aminopeptidase	-	
3.4.21.19	glutamyl endopeptidase	-	
3.4.21.82	Glutamyl endopeptidase II	-	
3.5.1.93	glutaryl-7-aminocephalosporanic-acid acylase	-	
3.4.19.13	glutathione gamma-glutamate hydrolase	-	
3.1.2.7	glutathione thiolesterase	-	
3.4.17.25	glutathione-S-conjugate glycine hydrolase	-	
3.5.1.78	Glutathionylspermidine amidase	-	
3.4.17.4	Gly-Xaa carboxypeptidase	-	
3.1.4.42	glycerol-1,2-cyclic-phosphate 2-phosphodiesterase	-	
3.1.3.21	glycerol-1-phosphatase	-	
3.1.3.19	glycerol-2-phosphatase	-	
3.6.3.20	glycerol-3-phosphate-transporting ATPase	transferred to EC 7.6.2.10	
3.1.4.38	glycerophosphocholine cholinephosphodiesterase	-	
3.1.4.2	glycerophosphocholine phosphodiesterase	-	
3.1.4.46	glycerophosphodiester phosphodiesterase	-	
3.1.4.44	glycerophosphoinositol glycerophosphodiesterase	-	
3.1.4.43	glycerophosphoinositol inositolphosphodiesterase	-	
3.2.2.18	glycopeptide N-glycosidase	deleted, included in EC 3.5.1.52	
3.2.1.130	glycoprotein endo-alpha-1,2-mannosidase	-	
3.5.1.69	glycosphingolipid deacylase	-	
3.1.6.3	glycosulfatase	-	
3.2.1.62	glycosylceramidase	-	
3.1.4.50	glycosylphosphatidylinositol phospholipase D	-	
3.4.22.25	glycyl endopeptidase	-	
3.4.3.1	glycyl-glycine dipeptidase	transferred to EC 3.4.13.18	
3.4.13.1	glycyl-glycine dipeptidase	transferred to EC 3.4.13.18	
3.4.3.2	glycyl-leucine dipeptidase	transferred to EC 3.4.13.18	
3.4.13.2	glycyl-leucine dipeptidase	transferred to EC 3.4.13.18	
3.2.1.B44	glycyrrhizin glucuronohydrolase (glycyrrhetinic acid 3-O-beta-D-glucuronide-forming)	preliminary BRENDA-supplied EC number	
3.2.1.128	glycyrrhizin hydrolase	-	
3.4.24.78	gpr endopeptidase	-	
3.4.21.78	granzyme A	-	
3.4.21.79	granzyme B	-	
3.4.21.B59	granzyme H	preliminary BRENDA-supplied EC number	
3.4.21.B4	granzyme K	preliminary BRENDA-supplied EC number	
3.4.21.B2	granzyme M	preliminary BRENDA-supplied EC number	
3.5.4.16	GTP cyclohydrolase I	-	
3.5.4.25	GTP cyclohydrolase II	-	
3.5.4.29	GTP cyclohydrolase IIa	-	
3.5.4.39	GTP cyclohydrolase IV	-	
3.5.3.2	guanidinoacetase	-	
3.5.3.7	guanidinobutyrase	-	
3.1.3.40	guanidinodeoxy-scyllo-inositol-4-phosphatase	-	
3.5.3.17	Guanidinopropionase	-	
3.5.4.3	guanine deaminase	-	
3.6.3.37	guanine-transporting ATPase	transferred to EC 7.6.2.6	
3.5.4.15	guanosine deaminase	-	
3.1.7.2	guanosine-3',5'-bis(diphosphate) 3'-diphosphatase	-	
3.6.1.B16	guanosine-3',5'-bis(diphosphate) bisphosphatase	preliminary BRENDA-supplied Ec number	
3.1.11.8	guanosine-5'-diphospho-5'-[DNA] diphosphatase	transferred to EC 3.6.1.70	
3.6.1.70	guanosine-5'-diphospho-5'-[DNA] diphosphatase	-	
3.6.1.40	guanosine-5'-triphosphate,3'-diphosphate phosphatase	-	
3.6.1.42	guanosine-diphosphatase	-	
3.6.3.6	H+-exporting ATPase	transferred to EC 7.1.2.1	
3.6.1.34	H+-transporting ATP synthase	transferred to  EC 7.1.2.2, H+-transporting two-sector ATPase	
3.6.1.35	H+-transporting ATPase	transferred to EC 3.6.3.6	
3.6.3.14	H+-transporting two-sector ATPase	transferred to EC 7.1.2.2	
3.6.1.36	H+/K+ exchanging ATPase	transferred to EC 3.6.3.10	
3.6.3.10	H+/K+-exchanging ATPase	transferred to EC 7.2.2.19	
3.8.1.3	haloacetate dehalogenase	-	
3.8.1.5	haloalkane dehalogenase	-	
3.1.99.B3	Hef endonuclease	preliminary BRENDA-supplied EC number	
3.4.22.45	helper-component proteinase	-	
3.6.3.41	heme-transporting ATPase	transferred to EC 7.6.2.5	
3.4.21.98	hepacivirin	-	
3.2.1.166	heparanase	-	
3.2.1.19	heparinase	deleted	
3.3.2.7	hepoxilin-epoxide hydrolase	-	
3.4.21.106	hepsin	-	
3.2.1.168	hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase	-	
3.6.1.46	heterotrimeric G-protein GTPase	transferred to EC 3.6.5.1	
3.6.5.1	heterotrimeric G-protein GTPase	-	
3.4.21.B42	hippostasin	preliminary BRENDA-supplied EC number	
3.5.1.32	hippurate hydrolase	-	
3.1.3.15	histidinol-phosphatase	-	
3.4.22.35	Histolysain	-	
3.5.1.98	histone deacetylase	-	
3.4.23.16	HIV-1 retropepsin	-	
3.4.23.47	HIV-2 retropepsin	-	
3.4.13.13	homocarnosinase	transferred to EC 3.4.13.3	
3.1.1.79	hormone-sensitive lipase	-	
3.4.24.47	horrilysin	-	
3.4.25.2	HslU-HslV peptidase	-	
3.4.21.108	HtrA2 peptidase	-	
3.4.23.50	human endogenous retrovirus K endopeptidase	-	
3.4.23.B8	human T-cell leukemia virus type 1 protease	preliminary BRENDA-supplied EC number	
3.4.99.9	hurain	deleted	
3.4.21.B1	hyaluronan-binding serine protease	preliminary BRENDA-supplied EC number	
3.2.1.35	hyaluronoglucosaminidase	-	
3.2.1.36	hyaluronoglucuronidase	-	
3.1.4.34	hybrid nuclease	deleted	
3.4.23.B20	HycD peptidase	preliminary BRENDA-supplied EC number	
3.4.23.51	HycI peptidase	-	
3.1.2.6	hydroxyacylglutathione hydrolase	-	
3.1.1.22	hydroxybutyrate-dimer hydrolase	-	
3.5.4.43	hydroxydechloroatrazine ethylaminohydrolase	-	
3.5.99.3	hydroxydechloroatrazine ethylaminohydrolase	transferred to EC 3.5.4.43	
3.5.2.17	hydroxyisourate hydrolase	-	
3.1.2.5	hydroxymethylglutaryl-CoA hydrolase	-	
3.4.21.49	hypodermin C	-	
3.4.24.B19	i-AAA protease	preliminary BRENDA-supplied EC number	
3.1.6.13	iduronate-2-sulfatase	-	
3.4.24.13	IgA-specific metalloendopeptidase	-	
3.4.21.72	IgA-specific serine endopeptidase	-	
3.5.2.7	imidazolonepropionase	-	
3.4.3.7	imidodipeptidase, prolidase	transferred to EC 3.4.13.9	
3.4.3.6	iminodipeptidase	transferred to EC 3.4.13.18	
3.5.4.10	IMP cyclohydrolase	-	
3.1.3.99	IMP-specific 5'-nucleotidase	-	
3.4.21.115	infectious pancreatic necrosis birnavirus Vp4 peptidase	-	
3.6.1.1	inorganic diphosphatase	-	
3.2.2.12	inosinate nucleosidase	-	
3.6.1.64	inosine diphosphate phosphatase	-	
3.2.2.2	inosine nucleosidase	-	
3.6.1.73	inosine/xanthosine triphosphatase	-	
3.6.1.B18	inositol diphosphate 1-phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.65	inositol-1,3-bisphosphate 3-phosphatase	transferred to EC 3.1.3.64	
3.1.3.61	inositol-1,4,5-trisphosphate 1-phosphatase	deleted	
3.1.3.57	inositol-1,4-bisphosphate 1-phosphatase	-	
3.1.3.25	inositol-phosphate phosphatase	-	
3.1.3.56	inositol-polyphosphate 5-phosphatase	-	
3.3.1.B1	inosylhomocysteinase	deleted, replaced by EC 3.13.2.B1	
3.13.2.B1	inosylhomocysteinase	preliminary BRENDA-supplied EC number	
3.4.22.11	insulinase	transferred to EC 3.4.99.45	
3.4.99.10	insulinase	transferred to EC 3.4.24.56	
3.4.99.45	insulinase	transferred to EC 3.4.24.56	
3.4.24.56	insulysin	-	
3.4.11.26	intermediate cleaving peptidase 55	-	
3.4.24.7	interstitial collagenase	-	
3.2.1.7	inulinase	-	
3.2.1.157	iota-carrageenase	-	
3.2.1.B16	ipecoside beta-D-glucosidase	preliminary BRENDA-supplied EC number	
3.1.1.108	iron(III)-enterobactin esterase	-	
3.1.1.109	iron(III)-salmochelin esterase	-	
3.6.3.34	iron-chelate-transporting ATPase	transferred to EC 7.2.2.16, EC 7.2.2.17, EC 7.2.2.18	
3.5.2.20	isatin hydrolase	-	
3.1.1.112	isoamyl acetate esteras	-	
3.2.1.68	isoamylase	-	
3.3.2.1	isochorismatase	-	
3.2.1.205	isomaltose glucohydrolase	-	
3.2.1.57	isopullulanase	-	
3.1.7.9	isotuberculosinol synthase	transferred to EC 2.5.1.153	
3.4.24.73	jararhagin	-	
3.5.1.127	jasmonoyl-L-amino acid hydrolase	-	
3.1.1.59	juvenile-hormone esterase	-	
3.6.3.12	K+-transporting ATPase	transferred to EC 7.2.2.6	
3.4.4.21	kallikrein	transferred to EC 3.4.21.34 and EC 3.4.21.35	
3.4.21.8	kallikrein	transferred to EC 3.4.21.34, EC 3.4.21.35	
3.4.21.B41	kallikrein 10	preliminary BRENDA-supplied EC number	
3.4.21.B43	kallikrein 12	preliminary BRENDA-supplied EC number	
3.4.21.119	kallikrein 13	-	
3.4.21.B45	kallikrein 14	preliminary BRENDA-supplied EC number	
3.4.21.118	kallikrein 8	-	
3.4.21.B40	kallikrein 9	preliminary BRENDA-supplied EC number	
3.1.3.92	kanosamine-6-phosphate phosphatase	-	
3.2.1.83	kappa-carrageenase	-	
3.2.1.103	keratan-sulfate endo-1,4-beta-galactosidase	-	
3.2.1.B43	keratanase II	preliminary BRENDA-supplied Ec number	
3.4.4.25	keratinase	deleted	
3.4.21.61	Kexin	-	
3.6.4.B1	kinesin K16	preliminary BRENDA-supplied EC number	
3.2.1.B32	kojibiose hydrolase	deleted, replaced by EC 3.2.1.216	
3.2.1.216	kojibiose hydrolase	-	
3.4.21.B48	kumamolysin	preliminary BRENDA-supplied EC number	
3.7.1.3	kynureninase	-	
3.4.24.B24	L-alanine-D-glutamate endopeptidase	preliminary BRENDA-supplied EC number	
3.1.1.15	L-arabinonolactonase	-	
3.1.3.B9	L-galactose 1-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.93	L-galactose 1-phosphate phosphatase	-	
3.2.1.76	L-iduronidase	-	
3.5.2.11	L-lysine-lactamase	-	
3.4.22.46	L-peptidase	-	
3.5.1.101	L-proline amide hydrolase	-	
3.1.1.65	L-rhamnono-1,4-lactonase	-	
3.2.1.108	lactase	-	
3.2.1.140	lacto-N-biosidase	-	
3.4.21.96	Lactocepin	-	
3.2.1.162	lambda-carrageenase	-	
3.4.99.36	leader peptidase	transferred to EC 3.4.21.89	
3.4.22.34	Legumain	-	
3.4.22.B47	legume cysteine endopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.36	leishmanolysin	-	
3.4.24.5	lens neutral proteinase	deleted, included in EC 3.4.22.17, EC 3.4.25.1	
3.4.1.1	leucine aminopeptidase	transferred to EC 3.4.11.1	
3.4.24.6	leucolysin	-	
3.4.11.1	leucyl aminopeptidase	-	
3.4.21.57	Leucyl endopeptidase	-	
3.4.99.42	leucyllysine endopeptidase	deleted	
3.4.21.37	leukocyte elastase	-	
3.4.21.51	leukocyte-membrane neutral endopeptidase	deleted	
3.3.2.6	leukotriene-A4 hydrolase	-	
3.4.19.14	leukotriene-C4 hydrolase	-	
3.2.1.65	levanase	-	
3.2.1.5	licheninase	deleted	
3.2.1.73	licheninase	-	
3.2.1.196	limit dextrin alpha-1,6-maltotetraose-hydrolase	-	
3.2.1.142	limit dextrinase	-	
3.3.2.8	limonene-1,2-epoxide hydrolase	-	
3.1.1.36	limonin-D-ring-lactonase	-	
3.4.21.86	Limulus clotting enzyme	-	
3.4.21.85	limulus clotting factor B	-	
3.4.21.84	limulus clotting factor C	-	
3.1.6.21	linear primary-alkylsulfatase	-	
3.1.3.76	lipid-phosphate phosphatase	-	
3.6.3.39	lipopolysaccharide-transporting ATPase	transferred to EC 7.5.2.5	
3.1.1.34	lipoprotein lipase	-	
3.5.1.55	long-chain-fatty-acyl-glutamate deacylase	-	
3.4.23.13	Lotus aspartic proteinase	deleted	
3.4.21.121	Lys-Lys/Arg-Xaa endopeptidase	-	
3.4.17.3	lysine carboxypeptidase	-	
3.1.1.5	lysophospholipase	-	
3.3.2.2	lysoplasmalogenase	-	
3.4.16.2	lysosomal Pro-Xaa carboxypeptidase	-	
3.4.24.75	lysostaphin	-	
3.2.1.17	lysozyme	-	
3.4.21.50	lysyl endopeptidase	-	
3.4.24.B18	m-AAA protease	preliminary BRENDA-supplied EC number	
3.6.1.30	m7G(5')pppN diphosphatase	deleted, now covered by EC 3.6.1.59 [m7GpppX diphosphatase] and EC 3.6.1.62 [m7GpppN-mRNA hydrolase]	
3.4.24.65	macrophage elastase	-	
3.4.24.62	magnolysin	-	
3.4.24.B25	MAHCE	preliminary BRENDA-supplied EC number	
3.5.1.107	maleamate amidohydrolase	-	
3.5.2.16	maleimide hydrolase	-	
3.7.1.23	maleylpyruvate hydrolase	-	
3.2.1.B8	malto-alpha-amylase (reducing end)	preliminary BRENDA-supplied EC number	
3.1.3.90	maltose 6'-phosphate phosphatase	-	
3.2.1.122	maltose-6'-phosphate glucosidase	-	
3.2.1.B36	maltose-forming alpha-amylase	preliminary BRENDA-supplied EC number	
3.6.3.19	maltose-transporting ATPase	transferred to EC 7.5.2.1	
3.5.1.86	mandelamide amidase	-	
3.6.3.35	manganese-transporting ATPase	transferred to EC 7.2.2.5	
3.2.1.77	mannan 1,2-(1,3)-alpha-mannosidase	-	
3.2.1.100	mannan 1,4-mannobiosidase	-	
3.2.1.78	mannan endo-1,4-beta-mannosidase	-	
3.2.1.101	mannan endo-1,6-alpha-mannosidase	-	
3.2.1.137	mannan exo-1,2-1,6-alpha-mannosidase	-	
3.4.21.B7	mannan-binding lectin-associated serine protease 1	preliminary BRENDA-supplied EC number	
3.4.21.104	mannan-binding lectin-associated serine protease-2	-	
3.1.3.22	mannitol-1-phosphatase	-	
3.1.3.70	mannosyl-3-phosphoglycerate phosphatase	-	
3.2.1.96	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase	-	
3.2.1.113	mannosyl-oligosaccharide 1,2-alpha-mannosidase	-	
3.2.1.114	mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase	-	
3.2.1.207	mannosyl-oligosaccharide alpha-1,3-glucosidase	-	
3.2.1.106	mannosyl-oligosaccharide glucosidase	-	
3.1.3.79	mannosylfructose-phosphate phosphatase	-	
3.1.3.B8	mannosylglucosyl-3-phosphoglycerate phosphatase	preliminary BRENDA-supplied EC number	
3.2.1.170	mannosylglycerate hydrolase	-	
3.2.1.152	mannosylglycoprotein endo-beta-mannosidase	-	
3.4.23.B6	Mason-Pfizer monkey virus proteinase	preliminary BRENDA-supplied EC number	
3.4.21.B5	mast cell protease 5	preliminary BRENDA-supplied EC number	
3.4.24.23	matrilysin	-	
3.4.21.109	matriptase	-	
3.4.24.B3	matrix metalloproteinase-11	preliminary BRENDA-supplied EC number	
3.4.24.B4	matrix metalloproteinase-13	preliminary BRENDA-supplied EC number	
3.4.24.B5	matrix metalloproteinase-15	preliminary BRENDA-supplied EC number	
3.4.24.B6	matrix metalloproteinase-20	preliminary BRENDA-supplied EC number	
3.4.24.B7	matrix metalloproteinase-26	preliminary BRENDA-supplied EC number	
3.5.4.45	melamine deaminase	-	
3.4.23.45	memapsin 1	-	
3.4.23.46	memapsin 2	-	
3.4.11.2	membrane alanyl aminopeptidase	-	
3.4.13.19	membrane dipeptidase	-	
3.4.17.16	membrane Pro-Xaa carboxypeptidase	-	
3.4.24.80	membrane-type matrix metalloproteinase-1	-	
3.4.24.18	meprin A	-	
3.4.24.63	meprin B	-	
3.4.13.12	Met-Xaa dipeptidase	-	
3.4.24.B39	metallo-alpha-fibrinogenase	preliminary BRENDA-supplied EC number	
3.4.17.22	metallocarboxypeptidase D	-	
3.5.4.9	methenyltetrahydrofolate cyclohydrolase	-	
3.5.4.27	methenyltetrahydromethanopterin cyclohydrolase	-	
3.4.11.18	methionyl aminopeptidase	-	
3.1.1.114	methyl acetate hydrolase	-	
3.1.1.97	methylated diphthine methylhydrolase	-	
3.5.3.21	methylenediurea deaminase	-	
3.5.3.16	methylguanidinase	-	
3.1.3.14	methylphosphothioglycerate phosphatase	-	
3.2.2.16	methylthioadenosine nucleosidase	-	
3.1.1.56	methylumbelliferyl-acetate deacetylase	-	
3.4.21.3	metridin	-	
3.4.99.14	mexicanain	deleted	
3.6.3.2	Mg2+-importing ATPase	transferred to EC 7.2.2.14	
3.4.23.6	microbial carboxyl proteinases	deleted, included in EC 3.4.23.18-28, EC 3.4.23.30	
3.4.24.3	microbial collagenase	-	
3.4.24.4	microbial metalloproteinases	transferred to EC 3.4.24.25-32, EC 3.4.24.39, EC 3.4.24.40	
3.4.21.14	microbial serine proteases	transferred to EC 3.4.21.62, EC 3.4.21.63, EC 3.4.21.64, EC 3.4.21.65, EC 3.4.21.66, EC 3.4.21.67	
3.1.4.7	micrococcal nuclease	transferred to EC 3.1.31.1	
3.1.31.1	micrococcal nuclease	-	
3.3.2.9	microsomal epoxide hydrolase	-	
3.6.4.3	microtubule-severing ATPase	transferred to EC 5.6.1.1	
3.5.1.61	mimosinase	-	
3.6.4.5	minus-end-directed kinesin ATPase	transferred to EC 5.6.1.4	
3.1.21.B3	mismatch-specific endonuclease	preliminary BRENDA-supplied EC number	
3.4.24.59	mitochondrial intermediate peptidase	-	
3.4.24.64	mitochondrial processing peptidase	-	
3.4.99.41	mitochondrial processing peptidase	transferred to EC 3.4.24.64	
3.6.3.51	mitochondrial protein-transporting ATPase	transferred to EC 7.4.2.3	
3.6.1.53	Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase	-	
3.6.3.29	molybdate-transporting ATPase	transferred to EC 7.3.2.5	
3.1.1.102	mono(ethylene terephthalate) hydrolase	-	
3.1.6.16	monomethyl-sulfatase	-	
3.6.3.17	monosaccharide-transporting ATPase	transferred to subgroup EC 7.5.2.	
3.1.1.83	monoterpene epsilon-lactone hydrolase	-	
3.1.7.3	monoterpenyl-diphosphatase	-	
3.4.23.B18	mouse mammary tumor virus retropepsin	preliminary BRENDA-supplied EC number	
3.6.1.74	mRNA 5'-phosphatase	-	
3.5.4.36	mRNA(cytosine6666) deaminase	-	
3.2.1.110	mucinaminylserine mucinaminidase	deleted, identical to EC 3.2.1.97, glycopeptide alpha-N-acetylgalactosaminidase	
3.4.23.23	Mucorpepsin	-	
3.4.24.54	mucrolysin	-	
3.4.99.46	multicatalytic endopeptidase complex	transferred to EC 3.4.25.1	
3.1.3.62	multiple inositol-polyphosphate phosphatase	-	
3.6.1.B11	multiple site diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase	preliminary BRENDA-supplied EC number	
3.4.17.8	muramoylpentapeptide carboxypeptidase	-	
3.4.17.13	Muramoyltetrapeptide carboxypeptidase	-	
3.4.23.B5	murine leukemia virus protease	preliminary BRENDA-supplied EC number	
3.4.24.B37	mutalysin II	preliminary BRENDA-supplied EC number	
3.2.1.61	mycodextranase	-	
3.4.24.31	mycolysin	-	
3.1.1.93	mycophenolic acid acyl-glucuronide esterase	-	
3.5.1.115	mycothiol S-conjugate amidase	-	
3.4.21.76	Myeloblastin	-	
3.6.1.32	myosin ATPase	transferred to EC 3.6.4.1	
3.6.4.1	myosin ATPase	transferred to EC 5.6.1.8	
3.4.24.B26	myroilysin	preliminary BRENDA-supplied EC number	
3.4.99.34	mytilidase	deleted	
3.4.99.29	Myxobacter AL-1 proteinase I	deleted	
3.4.99.30	Myxobacter AL-1 proteinase II	transferred to EC 3.4.24.20	
3.5.1.136	N,N'-diacetylchitobiose non-reducing end deacetylase	-	
3.5.1.56	N,N-dimethylformamidase	-	
3.5.1.60	N-(long-chain-acyl)ethanolamine deacylase	-	
3.5.1.103	N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase	-	
3.5.1.21	N-acetyl-beta-alanine deacetylase	-	
3.1.3.105	N-acetyl-D-muramate 6-phosphate phosphatase	-	
3.5.1.47	N-acetyldiaminopimelate deacetylase	-	
3.1.1.62	N-acetyldiaminopimelate deacylase	transferred to EC 3.5.1.47	
3.1.6.12	N-acetylgalactosamine-4-sulfatase	-	
3.5.1.80	N-acetylgalactosamine-6-phosphate deacetylase	deleted, identical to EC 3.5.1.25	
3.1.6.4	N-acetylgalactosamine-6-sulfatase	-	
3.1.1.58	N-acetylgalactosaminoglycan deacetylase	-	
3.5.1.33	N-acetylglucosamine deacetylase	-	
3.1.4.45	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase	-	
3.5.1.25	N-acetylglucosamine-6-phosphate deacetylase	-	
3.1.6.14	N-acetylglucosamine-6-sulfatase	-	
3.1.1.69	N-acetylglucosaminylphosphatidylinositol deacetylase	transferred to EC 3.5.1.89	
3.5.1.89	N-acetylglucosaminylphosphatidylinositol deacetylase	-	
3.4.19.4	N-acetylmethionylpeptide peptidase	deleted	
3.2.1.B37	N-acetylmuramoyl-glycoprotein endo-beta-N-acetylglucosaminidase	preliminary BRENDA-supplied EC number	
3.5.1.28	N-acetylmuramoyl-L-alanine amidase	-	
3.1.4.54	N-acetylphosphatidylethanolamine-hydrolysing phospholipase D	-	
3.5.1.14	N-acyl-aliphatic-L-amino acid amidohydrolase	-	
3.5.1.114	N-acyl-aromatic-L-amino acid amidohydrolase	-	
3.5.1.81	N-Acyl-D-amino-acid deacylase	-	
3.5.1.83	N-Acyl-D-aspartate deacylase	-	
3.5.1.82	N-acyl-D-glutamate deacylase	-	
3.1.3.29	N-acylneuraminate-9-phosphatase	-	
3.5.1.58	N-benzyloxycarbonylglycine hydrolase	-	
3.5.1.77	N-carbamoyl-D-amino-acid hydrolase	-	
3.5.1.87	N-carbamoyl-L-amino-acid hydrolase	-	
3.5.1.53	N-carbamoylputrescine amidase	-	
3.5.1.59	N-carbamoylsarcosine amidase	-	
3.5.1.71	N-feruloylglycine deacylase	-	
3.5.1.68	N-formylglutamate deformylase	-	
3.5.1.106	N-formylmaleamate deformylase	-	
3.4.19.7	N-Formylmethionyl-peptidase	-	
3.5.1.27	N-formylmethionylaminoacyl-tRNA deformylase	deleted, now covered by EC 3.5.1.88, peptide deformylase	
3.5.4.42	N-isopropylammelide isopropylaminohydrolase	-	
3.5.99.4	N-isopropylammelide isopropylaminohydrolase	transferred to EC 3.5.4.42	
3.5.1.95	N-malonylurea hydrolase	-	
3.2.2.25	N-methyl nucleosidase	-	
3.5.1.36	N-methyl-2-oxoglutaramate hydrolase	-	
3.5.1.137	N-methylcarbamate hydrolase	-	
3.5.2.14	N-methylhydantoinase (ATP-hydrolysing)	-	
3.5.1.91	N-substituted formamide deformylase	-	
3.5.3.23	N-succinylarginine dihydrolase	-	
3.10.1.1	N-sulfoglucosamine sulfohydrolase	-	
3.1.6.15	N-sulfoglucosamine-3-sulfatase	-	
3.5.3.24	N1-aminopropylagmatine ureohydrolase	-	
3.5.1.125	N2-acetyl-L-2,4-diaminobutanoate deacetylase	-	
3.4.13.15	N2-beta-alanylarginine dipeptidase	transferred to EC 3.4.13.18	
3.5.1.26	N4-(beta-N-acetylglucosaminyl)-L-asparaginase	-	
3.5.1.135	N4-acetylcytidine amidohydrolase	-	
3.5.1.129	N5-(cytidine 5'-diphosphoramidyl)-L-glutamine hydrolase	-	
3.6.3.7	Na+-exporting ATPase	transferred to EC 7.2.2.3	
3.6.3.15	Na+-transporting two-sector ATPase	transferred to EC 7.2.2.1	
3.6.1.37	Na+/K+ exchanging ATPase	transferred to EC 3.6.3.9	
3.6.3.9	Na+/K+-exchanging ATPase	transferred to EC 7.2.2.13	
3.6.1.22	NAD+ diphosphatase	-	
3.2.2.5	NAD+ glycohydrolase	-	
3.5.1.133	Nalpha-acyl-L-glutamine aminoacylase	-	
3.5.1.64	Nalpha-benzyloxycarbonylleucine hydrolase	-	
3.4.23.B1	napsin	preliminary BRENDA-supplied EC number	
3.4.24.61	nardilysin	-	
3.2.1.135	neopullulanase	-	
3.4.23.12	nepenthesin	-	
3.4.24.11	neprilysin	-	
3.4.24.B14	neprilysin-2	preliminary BRENDA-supplied EC number	
3.1.7.13	neryl diphosphate diphosphatase	-	
3.7.1.27	neryl diphosphate diphosphatase	transferred. Now EC 3.1.7.13	
3.4.24.16	neurolysin	-	
3.4.21.B10	neurosin	preliminary BRENDA-supplied EC number	
3.4.24.34	neutrophil collagenase	-	
3.6.3.24	nickel-transporting ATPase	transferred to EC 7.2.2.11	
3.5.1.19	nicotinamidase	-	
3.5.1.42	nicotinamide-nucleotide amidase	-	
3.6.3.26	nitrate-transporting ATPase	transferred to EC 7.3.2.4	
3.5.5.1	nitrilase	-	
3.2.2.14	NMN nucleosidase	-	
3.1.3.108	nocturnin	-	
3.4.23.44	nodavirus endopeptidase	-	
3.5.3.25	Nomega-hydroxy-L-arginine amidinohydrolase	-	
3.6.4.10	non-chaperonin molecular chaperone ATPase	-	
3.2.1.55	non-reducing end alpha-L-arabinofuranosidase	-	
3.2.1.185	non-reducing end beta-L-arabinofuranosidase	-	
3.2.1.88	non-reducing end beta-L-arabinopyranosidase	-	
3.4.13.17	non-stereospecific dipeptidase	-	
3.6.3.22	nonpolar-amino-acid-transporting ATPase	transferred to EC 7.4.2.2	
3.1.15.B1	NrnC nuclease	preliminary BRENDA-supplied EC number	
3.4.22.B79	nsP2 protease	preliminary BRENDA-supplied EC number	
3.4.22.44	nuclear-inclusion-a endopeptidase	-	
3.1.4.9	nucleate endonuclease	transferred to EC 3.1.30.2	
3.6.4.11	nucleoplasmin ATPase	deleted. The activity has been shown not to take place.	
3.6.1.6	nucleoside diphosphate phosphatase	-	
3.6.1.24	nucleoside phosphoacylhydrolase	-	
3.6.1.19	nucleoside-triphosphate diphosphatase	deleted, now included with EC 3.6.1.9	
3.6.1.15	nucleoside-triphosphate phosphatase	-	
3.1.3.31	nucleotidase	deleted. The activity may be that of an acid phosphatase.	
3.6.1.9	nucleotide diphosphatase	-	
3.1.1.106	O-acetyl-ADP-ribose deacetylase	-	
3.4.24.57	O-sialoglycoprotein endopeptidase	-	
3.1.2.14	oleoyl-[acyl-carrier-protein] hydrolase	-	
3.2.1.206	oleuropein beta-glucosidase	-	
3.2.1.10	oligo-1,6-glucosidase	-	
3.1.4.29	oligodeoxyribonucleate exonuclease	deleted	
3.4.24.B2	oligoendopeptidase F	preliminary BRENDA-supplied EC number	
3.1.4.19	oligonucleotidase	transferred to EC 3.1.13.3	
3.1.13.3	oligonucleotidase	-	
3.4.24.70	oligopeptidase A	-	
3.4.21.83	Oligopeptidase B	-	
3.6.3.23	oligopeptide-transporting ATPase	transferred to EC 7.4.2.6	
3.2.1.156	oligosaccharide reducing-end xylanase	-	
3.6.1.44	oligosaccharide-diphosphodolichol diphosphatase	-	
3.6.3.18	oligosaccharide-transporting ATPase	transferred to EC 7.5.2.2	
3.2.1.120	oligoxyloglucan beta-glycosidase	-	
3.2.1.150	oligoxyloglucan reducing-end-specific cellobiohydrolase	-	
3.5.1.3	omega-amidase	-	
3.4.21.87	omptin	transferred to EC 3.4.23.49	
3.4.23.49	omptin	-	
3.4.24.51	ophiolysin	-	
3.1.1.40	orsellinate-depside hydrolase	-	
3.4.21.63	Oryzin	-	
3.4.21.120	oviductin	-	
3.7.1.1	oxaloacetase	-	
3.5.1.126	oxamate amidohydrolase	-	
3.3.2.12	oxepin-CoA hydrolase	-	
3.7.1.16	oxepin-CoA hydrolase	transferred to EC 3.3.2.12	
3.4.99.28	Oxyuranus scutellatus prothrombin-activating proteinase	transferred to EC 3.4.21.60	
3.1.1.B10	p-coumaroyl esterase	preliminary BRENDA-supplied EC number	
3.4.21.B25	PACE4 proprotein convertase	preliminary BRENDA-supplied EC number	
3.4.99.15	Paecilomyces proteinase	deleted	
3.1.2.2	palmitoyl-CoA hydrolase	-	
3.1.2.22	palmitoyl[protein] hydrolase	-	
3.4.21.36	pancreatic elastase	-	
3.4.21.71	Pancreatic elastase II	-	
3.4.21.70	Pancreatic endopeptidase E	-	
3.1.27.5	pancreatic ribonuclease	transferred to EC 4.6.1.18	
3.4.4.7	pancreatopeptidase E	transferred to EC 3.4.21.36, EC 3.4.21.37	
3.5.1.92	pantetheine hydrolase	-	
3.5.1.22	pantothenase	-	
3.1.4.60	pApA phosphodiesterase	-	
3.4.4.10	papain	transferred to EC 3.4.22.2	
3.4.22.2	papain	-	
3.4.22.B14	papain-like proteinase 1	preliminary BRENDA-supplied EC number	
3.4.22.B50	papain-like proteinase 2	preliminary BRENDA-supplied EC number	
3.4.24.79	pappalysin-1	-	
3.1.1.11	pectinesterase	-	
3.5.1.11	penicillin amidase	-	
3.4.23.7	Penicillium janthinellum acid proteinase	transferred to EC 3.4.23.20	
3.4.99.16	Penicillium notatum extracellular proteinase	deleted	
3.4.23.20	Penicillopepsin	-	
3.5.1.50	pentanamidase	-	
3.4.11.23	PepB aminopeptidase	-	
3.4.15.2	pepdidyl carboxyamidase	transferred to EC 3.4.19.2	
3.4.4.1	pepsin	transferred to EC 3.4.23.1	
3.4.23.1	pepsin A	-	
3.4.4.2	pepsin B	transferred to EC 3.4.23.2	
3.4.23.2	pepsin B	-	
3.4.22.65	peptidase 1 (mite)	-	
3.4.21.107	peptidase Do	-	
3.4.21.64	peptidase K	-	
3.5.1.88	peptide deformylase	-	
3.5.1.52	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	-	
3.6.3.43	peptide-transporting ATPase	transferred to EC 7.4.2.5	
3.2.1.92	peptidoglycan beta-N-acetylmuramidase	-	
3.4.99.17	peptidoglycan endopeptidase	deleted	
3.5.1.104	peptidoglycan-N-acetylglucosamine deacetylase	-	
3.4.24.33	peptidyl-Asp metalloendopeptidase	-	
3.4.15.1	peptidyl-dipeptidase A	-	
3.4.15.4	Peptidyl-dipeptidase B	-	
3.4.15.5	Peptidyl-dipeptidase Dcp	-	
3.5.1.43	peptidyl-glutaminase	-	
3.4.19.2	peptidyl-glycinamidase	-	
3.4.24.20	peptidyl-Lys metalloendopeptidase	-	
3.4.21.B58	pernilase	preliminary BRENDA-supplied EC number	
3.4.21.B57	pernisine	preliminary BRENDA-supplied EC number	
3.6.4.7	peroxisome-assembly ATPase	-	
3.4.21.113	pestivirus NS3 polyprotein peptidase	-	
3.4.22.B20	PfpI endopeptidase	preliminary BRENDA-supplied EC number	
3.4.21.13	phaseolus proteinase	transferred to EC 3.4.16.1	
3.1.2.25	phenylacetyl-CoA hydrolase	-	
3.1.1.82	pheophorbidase	-	
3.4.24.B15	PHEX peptidase	preliminary BRENDA-supplied EC number	
3.7.1.4	phloretin hydrolase	-	
3.1.1.51	phorbol-diester hydrolase	-	
3.6.3.27	phosphate-transporting ATPase	transferred to EC 7.3.2.1	
3.1.3.4	phosphatidate phosphatase	-	
3.1.3.27	phosphatidylglycerophosphatase	-	
3.1.1.52	phosphatidylinositol deacylase	-	
3.1.3.67	phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase	-	
3.1.3.86	phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase	-	
3.1.3.66	phosphatidylinositol-3,4-bisphosphate 4-phosphatase	-	
3.1.3.95	phosphatidylinositol-3,5-bisphosphate 3-phosphatase	-	
3.1.3.64	phosphatidylinositol-3-phosphatase	-	
3.1.3.78	phosphatidylinositol-4,5-bisphosphate 4-phosphatase	-	
3.1.3.B4	phosphatidylinositol-4-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.1.111	phosphatidylserine sn-1 acylhydrolase	-	
3.6.2.2	phosphoadenylylsulfatase	-	
3.9.1.1	phosphoamidase	-	
3.1.4.1	phosphodiesterase I	-	
3.1.4.18	phosphodiesterase II	transferred to EC 3.1.16.1	
3.1.3.60	phosphoenolpyruvate phosphatase	-	
3.1.3.75	phosphoethanolamine/phosphocholine phosphatase	-	
3.1.3.20	phosphoglycerate phosphatase	-	
3.1.3.18	phosphoglycolate phosphatase	-	
3.9.1.3	phosphohistidine phosphatase	-	
3.1.3.36	phosphoinositide 5-phosphatase	-	
3.1.4.11	phosphoinositide phospholipase C	-	
3.1.1.32	phospholipase A1	-	
3.1.1.4	phospholipase A2	-	
3.1.4.3	phospholipase C	-	
3.1.4.4	phospholipase D	-	
3.1.1.118	phospholipid sn-1 acylhydrolase	-	
3.6.3.1	phospholipid-translocating ATPase	transferred to EC 7.6.2.1	
3.6.3.28	phosphonate-transporting ATPase	transferred to EC 7.3.2.2	
3.11.1.1	phosphonoacetaldehyde hydrolase	-	
3.11.1.2	phosphonoacetate hydrolase	-	
3.11.1.3	phosphonopyruvate hydrolase	-	
3.1.4.57	phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase	-	
3.1.4.55	phosphoribosyl 1,2-cyclic phosphate phosphodiesterase	-	
3.5.4.19	phosphoribosyl-AMP cyclohydrolase	-	
3.6.1.31	phosphoribosyl-ATP diphosphatase	-	
3.1.3.3	phosphoserine phosphatase	-	
3.5.1.79	Phthalyl amidase	-	
3.4.21.103	physarolisin	-	
3.4.23.27	physaropepsin	transferred to EC 3.4.21.103	
3.1.26.1	Physarum polycephalum ribonuclease	-	
3.4.23.40	Phytepsin	-	
3.4.22.29	picornain 2A	-	
3.4.22.28	picornain 3C	-	
3.1.1.85	pimelyl-[acyl-carrier protein] methyl ester esterase	-	
3.4.99.18	pinguinain	deleted	
3.4.24.55	pitrilysin	-	
3.4.99.44	pitrilysin	transferred to EC 3.4.24.55	
3.4.21.34	plasma kallikrein	-	
3.4.23.38	plasmepsin I	-	
3.4.23.39	plasmepsin II	-	
3.4.23.B14	plasmepsin IV	preliminary BRENDA-supplied EC number	
3.4.23.B19	plasmepsin V	preliminary BRENDA-supplied EC number	
3.4.4.14	plasmin	transferred to EC 3.4.21.7	
3.4.21.7	plasmin	-	
3.4.23.48	plasminogen activator Pla	-	
3.6.4.4	plus-end-directed kinesin ATPase	transferred to EC 5.6.1.3	
3.6.3.21	polar-amino-acid-transporting ATPase	transferred to EC 7.4.2.1	
3.1.1.75	poly(3-hydroxybutyrate) depolymerase	-	
3.1.1.76	poly(3-hydroxyoctanoate) depolymerase	-	
3.1.13.4	poly(A)-specific ribonuclease	-	
3.2.1.143	poly(ADP-ribose) glycohydrolase	-	
3.1.1.101	poly(ethylene terephthalate) hydrolase	-	
3.6.3.31	polyamine-transporting ATPase	transferred to EC 7.6.2.11	
3.2.1.121	polymannuronate hydrolase	-	
3.1.1.78	polyneuridine-aldehyde esterase	-	
3.1.3.32	polynucleotide 3'-phosphatase	-	
3.1.3.33	polynucleotide 5'-phosphatase	-	
3.4.23.29	Polyporopepsin	-	
3.1.27.B1	pre-mRNA splicing endonuclease EndA	preliminary BRENDA-supplied EC number	
3.4.23.52	preflagellin peptidase	-	
3.4.99.35	premurein-leader peptidase	transferred to EC 3.4.23.36	
3.1.7.1	prenyl-diphosphatase	-	
3.4.23.43	prepilin peptidase	-	
3.6.4.B4	presynaptic-filament-stabilizing ATPase	preliminary BRENDA-supplied EC number	
3.4.99.40	pro-gonadoliberin proteinase	deleted	
3.4.23.17	Pro-opiomelanocortin converting enzyme	-	
3.4.99.38	pro-opiomelanotropin-converting proteinase	transferred to EC 3.4.23.17	
3.4.24.89	Pro-Pro endopeptidase	-	
3.4.13.8	Pro-X dipeptidase	transferred to EC 3.4.13.18	
3.5.3.22	proclavaminate amidinohydrolase	-	
3.4.24.19	procollagen C-endopeptidase	-	
3.4.24.14	procollagen N-endopeptidase	-	
3.4.21.58	prohormone serine proteinase	deleted	
3.4.1.4	proline iminopeptidase	transferred to EC 3.4.11.5	
3.4.11.5	prolyl aminopeptidase	-	
3.4.22.18	prolyl endopeptidase (thiol-dependent)	transferred to EC 3.4.21.26	
3.4.21.26	prolyl oligopeptidase	-	
3.4.21.93	Proprotein convertase 1	-	
3.4.21.94	proprotein convertase 2	-	
3.4.21.B24	proprotein convertase 4	preliminary BRENDA-supplied EC number	
3.4.21.B26	proprotein convertase 5	preliminary BRENDA-supplied EC number	
3.4.21.B27	proprotein convertase 7	preliminary BRENDA-supplied EC number	
3.4.21.B12	prostase	preliminary BRENDA-supplied EC number	
3.4.21.B6	prostasin	preliminary BRENDA-supplied EC number	
3.4.24.B16	protease lasA	preliminary BRENDA-supplied EC number	
3.6.4.8	proteasome ATPase	transferred to EC 5.6.1.5	
3.4.25.1	proteasome endopeptidase complex	-	
3.9.1.2	protein arginine phosphatase	-	
3.4.21.69	Protein C (activated)	-	
3.5.1.124	protein deglycase	-	
3.5.1.121	protein N-terminal asparagine amidohydrolase	-	
3.5.1.122	protein N-terminal glutamine amidohydrolase	-	
3.2.1.169	protein O-GlcNAcase	-	
3.1.1.89	protein phosphatase methylesterase-1	-	
3.5.3.15	protein-arginine deiminase	-	
3.2.1.107	protein-glucosylgalactosylhydroxylysine glucosidase	-	
3.1.1.61	protein-glutamate methylesterase	-	
3.5.1.44	protein-glutamine glutaminase	-	
3.6.3.50	protein-secreting ATPase	transferred to EC 7.4.2.8	
3.1.3.16	protein-serine/threonine phosphatase	-	
3.6.1.48	protein-synthesizing GTPase	transferred to EC 3.6.5.3	
3.6.5.3	protein-synthesizing GTPase	-	
3.1.3.48	protein-tyrosine-phosphatase	-	
3.4.23.B13	proteinase P15	preliminary BRENDA-supplied EC number	
3.2.1.186	protodioscin 26-O-beta-D-glucosidase	-	
3.2.1.118	prunasin beta-glucosidase	-	
3.4.24.26	pseudolysin	-	
3.4.23.37	pseudomonapepsin	transferred to EC 3.4.21.100	
3.4.99.39	pseudomurein endopeptidase	deleted	
3.1.3.96	pseudouridine 5'-phosphatase	-	
3.5.4.11	pterin deaminase	-	
3.2.1.41	pullulanase	-	
3.5.1.119	Pup amidohydrolase	-	
3.2.2.1	purine nucleosidase	-	
3.4.23.30	Pycnoporopepsin	-	
3.5.1.B15	pyrazinamidase	preliminary BRENDA-supplied EC number	
3.1.1.88	pyrethroid hydrolase	-	
3.1.3.74	pyridoxal phosphatase	-	
3.2.2.10	pyrimidine-5'-nucleotide nucleosidase	-	
3.5.4.20	pyrithiamine deaminase	-	
3.4.21.B54	Pyrococcus abyssi serine endopeptidase	preliminary BRENDA-supplied EC number	
3.2.1.B28	Pyrococcus furiosus beta-glycosidase	preliminary BRENDA-supplied EC number	
3.4.17.B5	Pyrococcus furiosus carboxypeptidase	preliminary BRENDA-supplied EC number	
3.2.1.B40	Pyrococcus horikoshii beta-glycosidase	preliminary BRENDA-supplied EC number	
3.4.11.B4	Pyrococcus horikoshii deblocking aminopeptidase	preliminary BRENDA-supplied EC number	
3.4.21.B56	Pyrococcus horikoshii membrane protease PH1510	preliminary BRENDA-supplied EC number	
3.4.11.8	pyroglutamyl aminopeptidase	transferred to EC 3.4.19.3	
3.4.19.3	pyroglutamyl-peptidase I	-	
3.4.19.6	pyroglutamyl-peptidase II	-	
3.4.21.B55	pyrolysin	preliminary BRENDA-supplied EC number	
3.6.3.32	quaternary-amine-transporting ATPase	transferred to EC 7.6.2.9	
3.2.1.66	quercitrinase	deleted, reaction covered by EC 3.2.1.40	
3.1.1.81	quorum-quenching N-acyl-homoserine lactonase	-	
3.4.22.B15	rabbit hemorrhagic disease virus 3C-like protease	preliminary BRENDA-supplied EC number	
3.6.4.B9	Rad54 translocase	preliminary BRENDA-supplied EC number	
3.6.4.B7	RadA recombinase	preliminary BRENDA-supplied EC number	
3.2.1.125	raucaffricine beta-glucosidase	-	
3.4.99.B1	RCE1	preliminary BRENDA-supplied EC number	
3.4.99.37	RecA peptidase	deleted	
3.4.4.22	recommended Name never specified	transferred to EC 3.4.23.3	
3.4.4.23	recommended Name never specified	transferred to EC 3.4.23.5	
3.4.4.24	recommended Name never specified	transferred to EC 3.4.22.32, EC 3.4.22.33	
3.4.19.8	recommended Name never specified	transferred to EC 3.4.17.21	
3.4.21.24	red cell neutral endopeptidase	deleted	
3.4.4.15	renin	transferred to EC 3.4.23.15	
3.4.23.15	renin	-	
3.4.99.19	renin	transferred to EC 3.4.23.15	
3.4.4.3	rennin	transferred to EC 3.4.23.4	
3.4.21.88	Repressor LexA	-	
3.1.21.B2	restriction endonuclease PabI	preliminary BRENDA-supplied EC number	
3.1.1.64	retinoid isomerohydrolase	-	
3.1.1.21	retinyl-palmitate esterase	deleted, now covered by EC 3.1.1.1, carboxylesterase and EC 3.1.1.3, triacylglycerol lipase	
3.1.26.13	retroviral ribonuclease H	-	
3.1.1.86	rhamnogalacturonan acetylesterase	-	
3.2.1.173	rhamnogalacturonan galacturonohydrolase	-	
3.2.1.171	rhamnogalacturonan hydrolase	-	
3.2.1.174	rhamnogalacturonan rhamnohydrolase	-	
3.4.23.9	rhizopus acid proteinase	transferred to EC 3.4.23.21	
3.4.23.21	Rhizopuspepsin	-	
3.4.23.26	Rhodotorulapepsin	-	
3.4.21.105	rhomboid protease	-	
3.5.99.1	riboflavinase	-	
3.1.26.2	ribonuclease alpha	-	
3.1.13.5	ribonuclease D	-	
3.1.26.12	ribonuclease E	-	
3.1.27.7	ribonuclease F	-	
3.1.26.4	ribonuclease H	-	
3.1.4.22	ribonuclease I	transferred to EC 3.1.27.5	
3.1.4.23	ribonuclease II	transferred to EC 3.1.27.1	
3.1.26.3	ribonuclease III	-	
3.1.26.6	ribonuclease IV	-	
3.1.26.10	ribonuclease IX	-	
3.1.26.8	ribonuclease M5	-	
3.1.26.5	ribonuclease P	-	
3.1.26.7	ribonuclease P4	-	
3.1.27.3	ribonuclease T1	transferred to EC 4.6.1.24	
3.1.27.1	ribonuclease T2	transferred to EC 4.6.1.19	
3.1.27.4	ribonuclease U2	transferred to EC 4.6.1.20	
3.1.27.8	Ribonuclease V	-	
3.1.26.9	ribonuclease [poly-(U)-specific]	-	
3.2.1.148	ribosylhomocysteinase	transferred to EC 3.13.1.2	
3.3.1.3	ribosylhomocysteinase	transferred to EC 3.2.1.148	
3.2.2.8	ribosylpyrimidine nucleosidase	-	
3.5.5.2	ricinine nitrilase	-	
3.1.4.58	RNA 2',3'-cyclic 3'-phosphodiesterase	-	
3.6.4.13	RNA helicase	-	
3.4.23.B10	Rous sarcoma virus retropepsin	preliminary BRENDA-supplied EC number	
3.1.27.10	rRNA endonuclease	transferred to EC 4.6.1.23	
3.2.2.22	rRNA N-glycosylase	-	
3.4.24.48	ruberlysin	-	
3.4.24.58	russellysin	-	
3.1.2.9	S-acetoacetylhydrolipoate hydrolase	deleted	
3.1.2.8	S-acetoacylglutathione hydrolase	deleted, included in EC 3.1.2.6	
3.13.1.8	S-adenosyl-L-methionine hydrolase (adenosine-forming)	transferred, now EC 3.13.2.3	
3.3.1.2	S-adenosyl-L-methionine hydrolase (L-homoserine-forming)	transferred, now EC 3.13.2.2	
3.13.2.2	S-adenosyl-L-methionine hydrolase (L-homoserine-forming)	-	
3.5.4.28	S-adenosylhomocysteine deaminase	-	
3.1.2.12	S-formylglutathione hydrolase	-	
3.13.1.9	S-inosyl-L-homocysteine hydrolase	-	
3.5.4.31	S-methyl-5'-thioadenosine deaminase	-	
3.1.2.13	S-succinylglutathione hydrolase	-	
3.1.6.20	S-sulfosulfanyl-L-cysteine sulfohydrolase	-	
3.4.24.85	S2P endopeptidase	-	
3.4.24.37	saccharolysin	-	
3.4.23.25	saccharopepsin	-	
3.4.22.69	SARS coronavirus main proteinase	-	
3.4.22.B80	SARS-CoV papain-like protease	preliminary BRENDA-supplied EC number	
3.1.7.4	sclareol cyclase	deleted, now covered by EC 4.2.1.133 and EC 4.2.3.141	
3.4.99.20	scopulariopsis proteinase	deleted	
3.4.21.60	Scutelarin	-	
3.4.23.31	Scytalidopepsin A	-	
3.4.23.32	Scytalidopepsin B	-	
3.1.3.37	sedoheptulose-bisphosphatase	-	
3.4.21.100	sedolisin	-	
3.4.21.77	semenogelase	-	
3.4.22.B70	SENP1 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.B71	SENP2 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.B72	SENP3 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.B73	SENP5 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.B74	SENP6 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.B75	SENP7 peptidase	preliminary BRENDA-supplied EC number	
3.4.22.49	separase	-	
3.4.24.2	Sepia proteinase	deleted	
3.5.4.24	sepiapterin deaminase	-	
3.4.16.1	serine carboxypeptidase	transferred to EC 3.4.16.5, EC 3.4.16.6	
3.1.4.13	serine-ethanolaminephosphate phosphodiesterase	-	
3.4.16.4	serine-type D-Ala-D-Ala carboxypeptidase	-	
3.4.24.40	serralysin	-	
3.1.30.2	Serratia marcescens nuclease	-	
3.1.1.53	sialate O-acetylesterase	-	
3.4.21.89	Signal peptidase I	-	
3.4.23.36	Signal peptidase II	-	
3.4.23.B24	signal peptide peptidase	preliminary BRENDA-supplied EC number	
3.6.1.49	signal-recognition-particle GTPase	transferred to EC 3.6.5.4	
3.6.5.4	signal-recognition-particle GTPase	-	
3.4.23.B2	Simian immunodeficiency virus proteinase	preliminary BRENDA-supplied EC number	
3.1.1.49	sinapine esterase	-	
3.5.4.38	single-stranded DNA cytosine deaminase	-	
3.1.4.21	single-stranded-nucleate endonuclease	transferred to EC 3.1.30.1	
3.1.6.5	sinigrin sulfohydrolase; myrosulfatase	deleted	
3.4.21.112	site-1 protease	-	
3.6.1.47	small monomeric GTPase	transferred to EC 3.6.5.2	
3.6.5.2	small monomeric GTPase	-	
3.1.1.116	sn-1-specific diacylglycerol lipase	-	
3.1.3.B13	sn-2-glycerol-3-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.4.21.95	Snake venom factor V activator	-	
3.4.24.77	snapalysin	-	
3.4.99.21	solanain	deleted	
3.3.2.10	soluble epoxide hydrolase	-	
3.1.3.50	sorbitol-6-phosphatase	-	
3.4.23.14	sorghum aspartic proteinase	deleted	
3.4.22.70	sortase A	-	
3.4.22.71	sortase B	-	
3.4.22.B63	sortase C	preliminary BRENDA-supplied EC number	
3.4.22.B64	sortase D	preliminary BRENDA-supplied EC number	
3.4.21.99	spermosin	-	
3.5.1.109	sphingomyelin deacylase	-	
3.1.4.12	sphingomyelin phosphodiesterase	-	
3.1.4.41	sphingomyelin phosphodiesterase D	-	
3.1.16.1	spleen exonuclease	-	
3.4.21.116	SpoIVB peptidase	-	
3.4.23.B11	spumapepsin	preliminary BRENDA-supplied EC number	
3.4.24.B23	stage IV sporulation protein FB	preliminary BRENDA-supplied EC number	
3.4.22.48	staphopain	-	
3.4.22.13	staphylococcal cysteine proteinase	deleted	
3.4.99.22	staphylokinase	transferred to EC 3.4.24.29	
3.4.24.84	Ste24 endopeptidase	-	
3.4.22.32	Stem bromelain	-	
3.1.1.37	steroid-lactonase	-	
3.6.3.45	steroid-transporting ATPase	deleted, now included with EC 3.6.3.44, xenobiotic-transporting ATPase	
3.1.1.13	sterol esterase	-	
3.2.1.104	steryl-beta-glucosidase	-	
3.1.6.2	steryl-sulfatase	-	
3.4.21.117	stratum corneum chymotryptic enzyme	-	
3.4.21.B39	stratum corneum tryptic enzyme	preliminary BRENDA-supplied EC number	
3.4.4.18	streptococcus peptidase A	transferred to EC 3.4.22.10	
3.4.21.80	Streptogrisin A	-	
3.4.21.81	Streptogrisin B	-	
3.4.99.11	Streptomyces alkalophilic keratinase	deleted	
3.1.3.39	streptomycin-6-phosphatase	-	
3.4.22.10	streptopain	-	
3.5.2.19	streptothricin hydrolase	-	
3.4.24.17	stromelysin 1	-	
3.4.24.22	stromelysin 2	-	
3.4.21.40	submandibular proteinase A	deleted	
3.4.21.62	Subtilisin	-	
3.4.4.16	subtilopeptidase A	transferred to EC 3.4.21.62, EC 3.4.21.63, EC 3.4.21.64, EC 3.4.21.65, EC 3.4.21.66, EC 3.4.21.67	
3.1.2.3	succinyl-CoA hydrolase	-	
3.5.1.18	succinyl-diaminopimelate desuccinylase	-	
3.5.1.96	succinylglutamate desuccinylase	-	
3.2.1.B3	sucrose 6-phosphate hydrolase	preliminary BRENDA-supplied EC number	
3.2.1.48	sucrose alpha-glucosidase	-	
3.1.3.24	sucrose-phosphate phosphatase	-	
3.1.3.23	sugar-phosphatase	-	
3.1.3.58	sugar-terminal-phosphatase	-	
3.6.3.25	sulfate-transporting ATPase	transferred to EC 7.3.2.3	
3.2.1.B34	Sulfolobus acidocaldarius beta-glycosidase	preliminary BRENDA-supplied EC number	
3.2.1.B33	Sulfolobus shibatae beta-glycosidase	preliminary BRENDA-supplied EC number	
3.4.11.B3	Sulfolobus solfataricus aminopeptidase	preliminary BRENDA-supplied EC number	
3.2.1.B26	Sulfolobus solfataricus beta-glycosidase	preliminary BRENDA-supplied EC number	
3.4.17.B1	Sulfolobus solfataricus carboxypeptidase	preliminary BRENDA-supplied EC number	
3.4.24.B29	Sulfolobus solfataricus metalloendopeptidase	preliminary BRENDA-supplied EC number	
3.4.22.B77	Sulfolobus solfataricus peptidase	preliminary BRENDA-supplied EC number	
3.2.1.199	sulfoquinovosidase	-	
3.4.21.68	t-Plasminogen activator	-	
3.1.21.8	T4 deoxyribonuclease II	-	
3.1.21.9	T4 deoxyribonuclease IV	-	
3.4.99.23	tabernamontanain	deleted	
3.1.1.20	tannase	-	
3.6.3.36	taurine-transporting ATPase	transferred to EC 7.6.2.7	
3.1.1.103	teichoic acid D-alanine hydrolase	-	
3.6.3.40	teichoic-acid-transporting ATPase	transferred to EC 7.5.2.4	
3.4.21.18	Tenebrio alpha-proteinase	deleted	
3.4.99.24	Tenebrio alpha-proteinase	deleted	
3.4.24.68	tentoxilysin	-	
3.4.11.B9	TET2 peptidase	preliminary BRENDA-supplied EC number	
3.4.14.7	tetralysine endopeptidase	deleted	
3.12.1.B1	tetrathionate hydrolase	preliminary BRENDA-supplied EC number	
3.5.1.65	theanine hydrolase	-	
3.4.21.66	Thermitase	-	
3.2.1.B35	Thermococcus kodakaraensis beta-glycosidase	preliminary BRENDA-supplied EC number	
3.4.11.B6	Thermococcus kodakarensis deblocking aminopeptidase	preliminary BRENDA-supplied EC number	
3.4.17.B4	Thermococcus onnurineus carboxypeptidase	preliminary BRENDA-supplied EC number	
3.4.11.B5	Thermococcus onnurineus deblocking aminopeptidase	preliminary BRENDA-supplied EC number	
3.4.24.27	thermolysin	-	
3.4.21.65	Thermomycolin	-	
3.4.11.12	thermophilic aminopeptidase	deleted	
3.4.23.42	thermopsin	-	
3.4.99.43	thermopsin	transferred to EC 3.4.23.42	
3.4.23.B25	thermopsin-like protease SSO1175	preliminary BRENDA-supplied EC number	
3.4.21.B52	thermostable serine endopeptidase (Sulfolobus)	preliminary BRENDA-supplied EC number	
3.2.1.B41	Thermus thermophilus beta-glycosidase	preliminary BRENDA-supplied EC number	
3.1.3.100	thiamine phosphate phosphatase	-	
3.6.1.28	thiamine-triphosphatase	-	
3.4.24.15	thimet oligopeptidase	-	
3.5.5.8	thiocyanate hydrolase	-	
3.2.1.147	thioglucosidase	-	
3.2.3.1	thioglucosidase	transferred to EC 3.2.1.147	
3.4.4.13	thrombin	transferred to EC 3.4.21.5	
3.4.21.5	thrombin	-	
3.6.1.39	thymidine-triphosphatase	-	
3.1.3.35	thymidylate 5'-phosphatase	-	
3.2.2.29	thymine-DNA glycosylase	-	
3.4.23.11	thyroid aspartic proteinase	deleted	
3.8.1.4	thyroxine deiodinase	transferred to EC 1.97.1.10	
3.4.99.31	Tissue endopeptidase degrading collagenase synthetic substrate	transferred to EC 3.4.24.15	
3.4.21.35	tissue kallikrein	-	
3.4.21.90	Togavirin	-	
3.4.22.B8	Tpr proteinase (Porphyromonas gingivalis)	preliminary BRENDA-supplied EC number	
3.4.99.25	trametes acid proteinase	transferred to EC 3.4.23.21	
3.3.2.15	trans-2,3-dihydro-3-hydroxyanthranilic acid synthase	-	
3.3.2.4	trans-epoxysuccinate hydrolase	-	
3.4.21.B61	transmembrane protease serine 11D	preliminary BRENDA-supplied EC number	
3.4.21.B60	transmembrane protease serine 2	deleted, replaced by EC 3.4.21.122	
3.4.21.122	transmembrane protease serine 2	-	
3.1.3.12	trehalose-phosphatase	-	
3.4.21.B21	trepolisin	preliminary BRENDA-supplied EC number	
3.1.1.38	triacetate-lactonase	-	
3.1.1.3	triacylglycerol lipase	-	
3.4.24.10	Trichophyton mentagrophytes keratinase	deleted	
3.4.99.12	Trichophyton mentagrophytes keratinase	deleted	
3.4.24.9	Trichophyton schoenleinii collagenase	deleted	
3.4.21.B34	tricorn core protease (archaea)	preliminary BRENDA-supplied EC number	
3.4.24.52	trimerelysin I	-	
3.4.24.53	trimerelysin II	-	
3.6.1.2	trimetaphosphatase	-	
3.4.11.4	tripeptide aminopeptidase	-	
3.4.14.8	tripeptidyl peptidase	transferred to EC 3.4.14.9, EC 3.4.14.10	
3.4.14.9	tripeptidyl-peptidase I	-	
3.4.14.10	tripeptidyl-peptidase II	-	
3.6.1.25	triphosphatase	-	
3.12.1.1	trithionate hydrolase	-	
3.5.4.33	tRNA(adenine34) deaminase	-	
3.5.4.35	tRNA(cytosine8) deaminase	-	
3.1.27.9	tRNA-intron endonuclease	transferred to EC 4.6.1.16	
3.5.4.34	tRNAAla(adenine37) deaminase	-	
3.1.26.11	tRNase Z	-	
3.1.1.10	tropinesterase	-	
3.4.4.4	trypsin	transferred to EC 3.4.21.4	
3.4.21.4	trypsin	-	
3.4.21.59	Tryptase	-	
3.5.1.57	tryptophanamidase	-	
3.4.11.17	tryptophanyl aminopeptidase	-	
3.1.7.8	tuberculosinol synthase	transferred to EC 2.5.1.153	
3.6.1.51	tubulin GTPase	transferred to EC 3.6.5.6	
3.6.5.6	tubulin GTPase	-	
3.4.17.24	tubulin-glutamate carboxypeptidase	-	
3.4.17.17	tubulinyl-Tyr carboxypeptidase	-	
3.6.3.55	tungstate-importing ATPase	transferred to EC 7.3.2.6	
3.1.21.3	type I site-specific deoxyribonuclease	-	
3.1.21.4	type II site-specific deoxyribonuclease	-	
3.1.21.5	type III site-specific deoxyribonuclease	-	
3.4.16.3	tyrosine carboxypeptidase	transferred to EC 3.4.16.5	
3.4.21.73	u-Plasminogen activator	-	
3.1.2.15	ubiquitin thiolesterase	deleted, activity due to EC 3.4.19.12, ubiquitinyl hydrolase 1	
3.4.19.12	ubiquitinyl hydrolase 1	-	
3.6.1.54	UDP-2,3-diacylglucosamine diphosphatase	-	
3.6.1.57	UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase	-	
3.5.1.108	UDP-3-O-acyl-N-acetylglucosamine deacetylase	-	
3.1.4.B4	UDP-alpha-D-glucose glucohydrolase	preliminary BRENDA-supplied EC number	
3.2.1.184	UDP-N,N'-diacetylbacillosamine 2-epimerase (hydrolysing)	-	
3.2.1.183	UDP-N-acetylglucosamine 2-epimerase (hydrolysing)	-	
3.6.1.45	UDP-sugar diphosphatase	-	
3.13.1.1	UDP-sulfoquinovose synthase	-	
3.4.22.68	Ulp1 peptidase	-	
3.4.22.B67	Ulp2 peptidase	preliminary BRENDA-supplied EC number	
3.4.21.B30	UmuD protein	preliminary BRENDA-supplied EC number	
3.6.1.27	undecaprenyl-diphosphate phosphatase	-	
3.2.1.180	unsaturated chondroitin disaccharide hydrolase	-	
3.2.1.172	unsaturated rhamnogalacturonyl hydrolase	-	
3.2.2.27	uracil-DNA glycosylase	-	
3.5.1.5	urease	-	
3.5.1.45	urease (ATP-hydrolysing)	transferred to EC 6.3.4.6	
3.5.1.110	ureidoacrylate amidohydrolase	-	
3.5.1.116	ureidoglycolate amidohydrolase	-	
3.5.3.19	ureidoglycolate hydrolase	transferred to EC 3.5.1.116, ureidoglycolate amidohydrolase	
3.5.1.7	ureidosuccinase	-	
3.5.1.75	urethanase	-	
3.2.2.3	uridine nucleosidase	-	
3.4.21.31	urokinase	transferred to EC 3.4.21.68, EC 3.4.21.73	
3.4.99.26	urokinase	transferred to EC 3.4.21.7, EC 3.4.21.68	
3.1.1.19	uronolactonase	-	
3.4.22.50	V-cath endopeptidase	-	
3.1.3.101	validoxylamine A 7'-phosphate phosphatase	-	
3.1.4.47	variant-surface-glycoprotein phospholipase C	transferred to EC 4.6.1.14	
3.1.15.1	venom exonuclease	-	
3.4.21.74	venombin A	-	
3.4.21.55	Venombin AB	-	
3.1.1.94	versiconal hemiacetal acetate esterase	-	
3.6.4.6	vesicle-fusing ATPase	-	
3.4.24.25	vibriolysin	-	
3.2.1.119	vicianin beta-glucosidase	-	
3.4.22.B1	vignain	preliminary BRENDA-supplied EC number	
3.4.24.B34	Vipera ammodytes ammodytes metalloproteinase VaF1	preliminary BRENDA-supplied EC number	
3.4.24.B36	Vipera ammodytes ammodytes metalloproteinase VaH1	preliminary BRENDA-supplied EC number	
3.4.24.B33	Vipera ammodytes ammodytes metalloproteinase VaH3	preliminary BRENDA-supplied EC number	
3.4.24.B35	Vipera ammodytes ammodytes metalloproteinase VaH4	preliminary BRENDA-supplied EC number	
3.4.21.23	Vipera russelli proteinase	deleted	
3.1.1.12	vitamin A esterase	deleted	
3.6.3.33	vitamin B12-transporting ATPase	transferred to EC 7.6.2.8	
3.4.23.B17	walleye dermal sarcoma virus proteinase	preliminary BRENDA-supplied EC number	
3.1.1.50	wax-ester hydrolase	-	
3.4.13.4	Xaa-Arg dipeptidase	-	
3.4.13.3	Xaa-His dipeptidase	deleted, now covered by EC 3.4.13.18, cytosol nonspecific dipeptidase and EC 3.4.13.20, beta-Ala-His dipeptidase	
3.4.13.5	Xaa-methyl-His dipeptidase	-	
3.4.11.9	Xaa-Pro aminopeptidase	-	
3.4.13.9	Xaa-Pro dipeptidase	-	
3.4.14.11	Xaa-Pro dipeptidyl-peptidase	-	
3.4.11.16	Xaa-Trp aminopeptidase	-	
3.4.14.12	Xaa-Xaa-Pro tripeptidyl-peptidase	-	
3.4.21.101	xanthomonalisin	-	
3.4.23.33	xanthomonapepsin	transferred to EC 3.4.21.101	
3.6.3.44	xenobiotic-transporting ATPase	transferred to EC 7.6.2.2	
3.1.99.B2	XPF endonuclease	preliminary BRENDA-supplied EC number	
3.6.1.66	XTP/dITP diphosphatase	-	
3.2.1.72	xylan 1,3-beta-xylosidase	-	
3.2.1.37	xylan 1,4-beta-xylosidase	-	
3.2.1.131	xylan alpha-1,2-glucuronosidase	-	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	-	
3.2.1.155	xyloglucan-specific exo-beta-1,4-glucanase	-	
3.2.1.160	xyloglucan-specific exo-beta-1,4-glucanase	deleted, identical to EC 3.2.1.155	
3.1.1.68	xylono-1,4-lactonase	-	
3.1.1.110	xylono-1,5-lactonase	-	
3.4.23.41	yapsin 1	-	
3.4.2.3	yeast carboxypeptidase	transferred to EC 3.4.17.4	
3.4.22.22	yeast cysteine proteinase D	transferred to EC 3.4.24.37	
3.4.22.21	yeast cysteine proteinase E	transferred to EC 3.4.99.46	
3.4.22.23	yeast cysteine proteinase F	transferred to EC 3.4.21.61	
3.4.23.8	yeast proteinase A	transferred to EC 3.4.23.25	
3.4.22.9	yeast proteinase B	transferred to EC 3.4.21.48	
3.1.14.1	yeast ribonuclease	-	
3.4.22.B19	YopJ protease	preliminary BRENDA-supplied EC number	
3.1.1.B12	zearalenone hydrolase	preliminary BRENDA-supplied EC number	
3.4.17.14	Zinc D-Ala-D-Ala carboxypeptidase	-	
3.4.22.67	zingipain	-	
3.6.3.5	Zn2+-exporting ATPase	transferred to EC 7.2.2.12	
3.1.3.52	[3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]-phosphatase	-	
3.1.3.44	[acetyl-CoA carboxylase]-phosphatase	-	
3.1.4.14	[acyl-carrier-protein] phosphodiesterase	-	
3.5.1.130	[amino group carrier protein]-lysine hydrolase	-	
3.5.1.132	[amino group carrier protein]-ornithine hydrolase	-	
3.13.1.6	[CysO sulfur-carrier protein]-S-L-cysteine hydrolase	-	
3.1.3.42	[glycogen-synthase-D] phosphatase	-	
3.1.3.47	[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase	-	
3.4.14.14	[mycofactocin precursor peptide] peptidase	-	
3.1.3.53	[myosin-light-chain] phosphatase	-	
3.1.3.17	[phosphorylase] phosphatase	-	
3.2.2.19	[protein ADP-ribosylarginine] hydrolase	-	
3.2.2.B6	[protein ADP-ribosylaspartate] hydrolase	preliminary BRENDA-supplied EC number	
3.1.3.43	[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase	-	
3.1.3.49	[pyruvate kinase]-phosphatase	-	
3.1.1.98	[Wnt protein] O-palmitoleoyl-L-serine hydrolase	-