6.3.2.49	crystal structure of the enzyme in complex with ADP and an intermediate analog, phosphorylated phosphinate L-alanyl-L-phenylalanine, refined to 2.5 A resolution, sitting-drop vapor-diffusion method at 20°C	
6.3.2.49	purified recombinant wild-type and selenomethionine-labeled LAL BL00235s, sitting drop vapour diffusion method, mixing of 00.001 ml of protein solution consisting of 8 mg/ml protein, 1.6 mM ADP, 1.6 mM Met-Ala dipeptide, with 0.001 ml of reservoir solution containing 100 mM CaCl2, 24-27% w/v PEG MME 550, 4% v/v 2-propanol, and 100 mM imidazole, pH 6.5, and equilibration against 0.3 ml of reservoir solution, at room temperature, X-ray diffraction structure determination and analysis at 1.9 A and 1.6 A resolution, respectively, multi-wavelength anomalous dispersion method	
6.3.2.49	purified wild-type and selenomethionine-labeled YwfE in complex with ADP, MgCl2 and dipeptide L-Ala-L-Gln, hanging drop vapour diffusion method, mixing of 0.002 ml of protein solution containing 8 mg/ml protein, 10 mM ADP, 10 mM MgCl2, 100 mM L-Ala-L-Gln with 0.002 ml reservoir solution, equilibration against 0.5 ml of reservoir solution, the deletion-mutant crystals are grown in reservoir solution consisting of 17% w/v PEG 3350, 0.3 M NaCl, 0.1 M MES-NaOH, pH 6.75, 5% v/v ethylene glycol, 1 mM DTT, X-ray diffraction structure determination and analysis at 1.9 A and 2.8 A, respectively	
6.3.2.49	YwfE with bound ADP-Mg2+-phosphate and ADP-Mg2+-L-Ala, X-ray diffraction structure determination and analysis at 1.9 and 2.0 A resolution, respectively