2.5.1.9	crystallization of enzyme complexed with 6-carboxyethyl-7-oxo-8-ribityllumazine, sitting drop vapour diffusion method against equal amounts of reservoir solution containing 0.1 M bicine, pH 9.0, 65% v/v 2-mehyl-2,4-pentanediol, enzyme solution: 9 mg/ml, 20 mM TrisHCl, pH 7.0, 0.1 M KCl, 10 molar excess of solid 8, X-ray structure determination and analysis, structure model building and refinement, overview	637584	
2.5.1.9	crystals of wild-type enzyme are grown at 18°C using the sitting drop vapor diffusion method by mixing equal amounts of protein (6 mg/ml) in 100 mM potassium phosphate, pH 7.0, containing 30 mM Tris and 2 mM dithiothreitol with a reservoir solution containing 0.1 M HEPES, pH 7.0, and 40% 2-methyl-2,4-pentanediol. Crystal structures of the enzyme and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine	727855	
2.5.1.9	detailed structure determination and analysis of enzyme complexed with heavy atoms, three-dimensional structure model	637568	
2.5.1.9	from 1.3 M sodium/potassium phosphate, pH 8.7, 0.35 mM 5-nitroso-6-(1'-D-ribitylamino)-2,4(1H,3H)-pyrimidinedione, X-ray structure determination and analysis	637566	
2.5.1.9	hanging drop vapor diffusion method, using 12% (w/v) PEG 8000, 10% (w/v) glycerol, 0.5 M potassium chloride	737344	
2.5.1.9	large crystals by vapour diffusion method, initial solution: 0.7 M sodium/potassium phosphate, pH 8.7, 0.3 mM 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, protein 2 mg/ml, reservoir solution: 1.3 M sodium/potassium phosphate, pH 8.7, X-ray structure determination and analysis	637571	
2.5.1.9	modeling for the binding of two molecules of inhibitor 5-(4-phosphonobutyryl)amino-6-D-ribitylaminouracil in the active site	637586	
2.5.1.9	molecular modeling of inhibitors to the active site	637585	
2.5.1.9	purified recombinant homodimeric N-terminal enzyme domain in complex with riboflavin, 7 mg/ml protein in 70 mM sodium potassium phosphate, pH 7.0, and 100 mM sodium chloride, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular modeling	659699	
2.5.1.9	structure determination by multiwavelength anomalous diffraction method, modeling	637582