3.4.22.10 - 81451 3.4.22.10 analysis of a crystal structure model of the zymogen 667311 3.4.22.10 crystal structures are determined of mature apo-SpeB at 1.06 A resolution from a crystal obtained in neutral pH conditions and high resolution crystal structures of SpeB in complex with the general proteinase inhibitor trans-epoxysuccinyl- L-leucylamido(4-guanidino)butane (E64) and in complex with the SpeB-specific N-terminal acetylated peptide aldehyde inhibitor acetyl-Ala-Gln-Ile-(S)-2,6-diaminohexanal 734185 3.4.22.10 hanging drop vapour diffusion method, the crystal structure of the C47S mutant zymogen 40000 Da form expressed in Escherichia coli and its SeMet-substituted form, at 1.6 A resolution, reveals that the protein is a distant homologue of the papain superfamily 653648 3.4.22.10 purified recombinant mutant C47S, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution 668772