1.5.1.5	hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M ammonium acetate	
1.5.1.5	hanging drop vapour diffusion method, in the presence of its cofactor NADP+, at 22°C using polyethylene glycol 4000 as a precipitant	
1.5.1.5	native form and as a NADP complex, hanging drop vapor diffusion method, at 22°C in 18% PEG 4000, 400 mM NaCl, and 100 mM Tris-HCl (pH 8.0)	
1.5.1.5	purified recombinant bmMTFDH in complex with, X-ray diffraction structure determination and analysis at 1.75 A resolution	
1.5.1.5	three ternary complex structures of human methylenetetrahydrofolate dehydrogenase/cyclohydrolase, models are deposited in PDB following further refinement and with interpretation of the data