3.6.5.4 - 644172, 644173, 644178 3.6.5.4 X-ray structure of a complex of the N and G domains of Ffh with the GTPase FtsY of the SRP receptor in the presence of the non-hydrolyzable GTP analogue GMPPCP 660000 3.6.5.4 crystal structure of SRP54 with and without its cognate RNA binding site 660347 3.6.5.4 crystal structures of the complex of signal recognition particle and signal recogition particle receptor show that the two GTPases associate via an unusually extensive and highly cooperative interaction surface and form a composite active site at the interface 668629 3.6.5.4 hanging drop vapor diffusion method, 2.45 A crystal structure of the mammalian SRbeta in its Mg2+GTP-bound state in complex with the minimal binding domain of SRalpha termed SRX 669525 3.6.5.4 ammonium sulfate precipitation or sodium citrate precipitation, structures of the NG domain of FtsY in two different forms: an apo and a sulfate-loaded form 670045 3.6.5.4 crystal structure of the S-domain of signal-recognition-particle RNA at 2.6 A 670873 3.6.5.4 two structures of the SRP GTPase Ffh NG-domains are determined at 1.1 A resolution providing the basis for comparative examination of the extensive water structure of the apo conformation 684156 3.6.5.4 the crystal structure of PAB0955, free and in complex with six different nucleotides, is determined 686396 3.6.5.4 the crystal structure of cpFtsY with bound malonate is solved at 1.75 A resolution 686752 3.6.5.4 the crystal structure of cpFtsY at 2.0 A resolution is reported 688395 3.6.5.4 the structure of the GMPPNP-stabilized complex of Thermus aquaticus Ffh and FtsY NG domains is determined at 1.97 A resolution 688692 3.6.5.4 the 2.1 A X-ray structure of FtsY from Thermus aquaticus bound to GDP is reported 689926 3.6.5.4 hanging drop vapour diffusion method, at 21°C, using 50 mM cacodylic acid pH 6.5, 22% (w/v) PEG 4000, and 50 mM sodium acetate 695328 3.6.5.4 GDP-bound subunit SRP54 and free subunit SRP19 are crystallized by hanging drop vapour diffusion method, crystals of SRP54 grow in 1.0-1.3 M lithium sulfate and 100 mM sodium acetate pH 5.0, crystals of SRP19 grow in 1.2-1.3 M sodium malonate and 100 mM sodium acetate pH 5.0 700888 3.6.5.4 free and GDP-magnesium-bound forms, hanging drop vapour diffusion method, the hexagonal form grows in 1.1-1.5 M ammonium phosphate and 100 mM sodium acetate pH 5.0. The monoclinic form grows in 0.9-1.2 M lithium sulfate, 0.4-0.6 M ammonium sulfate, and 100 mM sodium citrate pH 5.0. For the GDP-bound structure, best crystals grow in 14-17% (w/v) PEG 8000 and 100 mM Tris pH 8.0 700889 3.6.5.4 subunit cpSRP43 in complex with a synthetic L18 peptide, hanging drop vapour diffusion method, using 701227 3.6.5.4 signal recognition particle in complex with its receptor, X-ray diffraction structure determination and analysis at 3.94 A resolution 721054 3.6.5.4 SRalpha and SRbeta complex, X-ray diffraction structure determination and analysis 757430 3.6.5.4 purified recombinant isolated FtsYNG domain in the nucleotide-free (apo) form, the GDP-bound, and the non-hydrolysable GTP-bound form (including GMPPNP (5'-guanylyl imidodiphosphate) and GMPPCP (beta,gamma-methyleneguanosine 5'-triphosphate)), X-ray diffraction structure determination and analysis at 1.22-1.88 A resolution 757514 3.6.5.4 purified recombinant SRbeta in complex with the SRX domain of SRalpha in the GTP-bound state and of GDP- and GDP-Mg2+-bound SRbeta, X-ray diffraction structure determination and analysis at 1.9-3.2 A resolution 758481