3.1.3.12	hanging drop vapor diffusion method, using 33% (w/v) PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol and 10 mM CoCl2	730793	
3.1.3.12	purified apo-active enzyme and trehalose-6-phosphate-complexed enzyme, vapor diffusion technique, mixing of 0.001 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 7.0, 50 mM NaCl, 1 mM MgCl2, and 10 mM DTT, with 0.001 ml of reservoir solution containing 20 mM sodium acetate trihydrate, pH 4.6, 50 mM CaCl2, 5% 2-propanol, 160 mM ammonium formate, and 16% PEG 3350, microseeding strategy, for substrate-bound crystals, soaking in 50 mM trehalose-6-phosphate for 6 to 12 h, 7 days, 20°C, X-ray diffraction structure detremination and analysis at 2.6 A and 1.7 A resolution, respectively	750507	
3.1.3.12	purified detagged recombinant selenomethionine-labeled or wild-type enzyme, hanging drop vapour diffusion method, from 0.2 M MgCl2, 20% PEG 6000, 0.1 M 2-(N-morpholino)ethane sulfonic acid, pH 6.0, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.9 A and 2.3 A resolution, respectively, and heavy atom derivatization using soaking procedures, molecular replacement	750510	
3.1.3.12	purified isolated N-terminal domain of Tps2 (Tps2NTD, residues 1-534), X-ray diffraction structure determination and analysis at 2.56 A resolution, molecular replacement using the structure of Escherichia coli OtsA, a Tps1 homologue, PDB ID code 1UQU, as a search model. Purified recombinant Tps2 C-terminal trehalose-6-phosphate phosphatase domain (Tps2PD) bound to BeF3 and trehalose (Tps2PD-BeF3-trehalose transition-state complex), X-ray diffraction structure determination and analysis at 2.0 A resolution	752047	
3.1.3.12	purified recombinant catalytically dead Tps2PD(D24N) from Cryptococcus neoformans bound to trehalose-6-phosphate, X-ray diffraction structure determination and analysis at 2.56 A resolution	752047	
3.1.3.12	sitting drop vapor diffusion crystallization of both the native and SeMet proteins	749237	
3.1.3.12	sitting drop vapour diffusion method with 30% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5)	682725