3.4.22.69 SARS coronavirus main proteinase 6e-18 - promazine in silico binding studies 7491 3.4.22.69 SARS coronavirus main proteinase 8.7e-17 - lopinavir in silico binding studies 1430 3.4.22.69 SARS coronavirus main proteinase 2.1e-16 - N-[3-[(E)-(tert-butoxyimino)methyl]-4-chlorophenyl]-2-methylfuran-3-carboxamide in silico binding studies 132814 3.4.21.5 thrombin 0.00000000000014 - RWJ-50353 - 54155 3.4.21.5 thrombin 0.0000000000011 - RWJ-51438 - 54154 1.5.1.3 dihydrofolate reductase 0.00000000002 - methotrexate recombinant enzyme 241 3.4.21.75 Furin 0.0000000000224 - 4-aminomethyl-phenylacetyl-Arg-Tle-Arg-4-aminomethyl-benzamidine pH and temperature not specified in the publication 239657 3.4.21.75 Furin 0.0000000000337 - Arg-Arg-Arg-Val-Arg-4-aminomethyl-benzamidine pH and temperature not specified in the publication 239655 3.2.2.16 methylthioadenosine nucleosidase 0.00000000004 - 5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201203 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000000047 - (3R,4S)-4-(4-chlorophenyl-thiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium complex of enzyme-inhibitor following slow-onset inhibition 35802 3.4.21.5 thrombin 0.0000000000493 - dipetalogastin II pH 8.0, 37°C 54150 3.2.2.16 methylthioadenosine nucleosidase 0.00000000011 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 3417 3.4.21.5 thrombin 0.000000000125 - inhibitor from Dipetalogaster maximus pH 8.0, 25°C 54142 3.4.21.5 thrombin 0.000000000237 - Hirudin pH 8.0, 37°C 3872 3.2.2.9 adenosylhomocysteine nucleosidase 0.0000000003 - (3R,4S)-4-(1-butylthiomethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine pH and temperature not specified in the publication 194385 3.2.2.16 methylthioadenosine nucleosidase 0.0000000003 - BuT-DADMe-ImmA pH and temperature not specified in the publication 201199 3.4.21.1 chymotrypsin 0.00000000035 - anti-trypsin-chymotrypsin A - 54062 3.4.21.1 chymotrypsin 0.0000000004 - anti-trypsin-chymotrypsin B - 54063 3.4.21.5 thrombin 0.000000000446 - dipetarudin pH 8.0, 37°C 54149 3.2.2.9 adenosylhomocysteine nucleosidase 0.00000000046 - (3R,4S)-4-(benzylthiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium complex of enzyme-inhibitor following slow-onset inhibition 8658 3.4.24.24 gelatinase A 0.00000000068 - APP-IP-TIMP-2 pH 7.5, 37°C 206301 3.4.22.38 cathepsin K 0.0000000008 - Stefin B Y67L/L205A mutant cathepsin K 12910 3.4.23.16 HIV-1 retropepsin 0.0000000008 - (5S)-3-(3-acetylphenyl)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild-type protein 14963 3.2.1.3 glucan 1,4-alpha-glucosidase 0.000000001 - acarbose - 412 3.4.23.16 HIV-1 retropepsin 0.000000001 - tipranavir D30N/L63P/N88D mutant protein 2316 3.4.22.1 cathepsin B 0.0000000011 - Z-Arg-Leu-His-Agly-Ile-Val-OMe - 60866 3.5.1.5 urease 0.0000000012 - N-[(2,3-dichlorophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide pH 8.2, 37°C, competitive inhibition, irreversible 238884 1.2.1.4 aldehyde dehydrogenase (NADP+) 0.0000000014 - PCMB - 78 3.4.22.15 cathepsin L 0.00000000149 - p41-fragment-human substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at least 10-fold higher than enzyme concentration) 18965 3.4.22.34 Legumain 0.0000000016 - human cystatin E/M pH 5.6, 37°C 54352 3.4.22.15 cathepsin L 0.0000000018 - chagasin mutant T31A/T32A - 26688 1.5.1.3 dihydrofolate reductase 0.000000002 - methotrexate pH 7.4, 37°C, mutant Q35K 241 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000002 - (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium complex of enzyme-inhibitor following slow-onset inhibition 11566 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000002 - (1S)-5-(4-chlorophenylthio)-1-(9-deaza-adenin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol 25°C, pH 7.0,dissociation constant for the equilibrium complex of enzyme-inhibitor following slow-onset inhibition 35799 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000002 - (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]3-hydroxy-4-methylthiomethylpyrrolidine pH and temperature not specified in the publication 194384 3.2.2.16 methylthioadenosine nucleosidase 0.000000002 - (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-methylthiomethylpyrrolidine pH and temperature not specified in the publication 62471 3.2.2.30 aminodeoxyfutalosine nucleosidase 0.000000002 - (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]3-hydroxy-4-methylthiomethylpyrrolidine pH and temperature not specified in the publication 194384 3.4.23.16 HIV-1 retropepsin 0.000000002 - N2-acetyl-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(pentyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-L-valinamide wild-type protein 14959 2.4.2.1 purine-nucleoside phosphorylase 0.0000000021 - 2-amino-7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one - 76551 3.5.1.5 urease 0.0000000025 - 2-[4-(2-methylpropyl)phenyl]-N-[(3-nitrophenyl)carbamothioyl]propanamide pH 8.2, 37°C, mixed-type inhibition 238222 3.2.2.16 methylthioadenosine nucleosidase 0.0000000027 - 5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201203 3.4.22.15 cathepsin L 0.0000000027 - chagasin mutant T32Y - 26686 3.4.23.16 HIV-1 retropepsin 0.0000000027 - (3aS,4S,7aR)-hexahydro-4H-furo[2,3-b]pyran-4-yl [(2S,3R)-3-hydroxy-4-{[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino}-1-phenylbutan-2-yl]carbamate pH and temperature not specified in the publication 41895 1.5.1.3 dihydrofolate reductase 0.000000003 - methotrexate pH 7.4, 37°C, mutant N64S 241 3.4.17.21 Glutamate carboxypeptidase II 0.000000003 - (S)-2-(3-((S)-1-carboxy-(4-iodobenzamido)pentyl)ureido)pentanedioic acid pH and temperature not specified in the publication 75334 3.4.21.62 Subtilisin 0.000000003 - chymotrypsin inhibitor - 57038 3.4.23.16 HIV-1 retropepsin 0.000000003 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-3-[3-(methylsulfonyl)phenyl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41886 3.5.1.5 urease 0.000000003 - 2-[4-(2-methylpropyl)phenyl]-N-[(2,4,6-trimethylphenyl)carbamothioyl]propanamide pH 8.2, 37°C, mixed-type inhibition 238221 3.4.21.62 Subtilisin 0.0000000033 - chymotrypsin inhibitor 2 mutant M59Y - 60803 2.4.2.1 purine-nucleoside phosphorylase 0.0000000034 - 2-amino-7-([[(2R,3S)-1,3,4-trihydroxybutan-2-yl]amino]methyl)-3,5-dihydro-4Hpyrrolo[3,2-d]pyrimidin-4-one - 76549 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.0000000034 - (3R,4S)-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28435 3.4.21.62 Subtilisin 0.0000000036 - human proteinase inhibitor 9 pH 7.5, 25°C 54162 3.4.22.15 cathepsin L 0.0000000039 - chagasin mutant T31A - 26680 3.4.23.16 HIV-1 retropepsin 0.000000004 - (2S)-N-[(1S,2R,3S,4S)-1-benzyl-2,3-dihydroxy-4-[[(2S)-3-methyl-2-[[methyl(pyridin-2-ylmethyl)carbamoyl]amino]butanoyl]amino]-5-phenylpentyl]-3-methyl-2-[[methyl(pyridin-2-ylmethyl)carbamoyl]amino]butanamide - 65106 3.4.23.16 HIV-1 retropepsin 0.000000004 - (5S)-3-(4-acetylphenyl)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild-type protein 14965 3.4.22.38 cathepsin K 0.0000000041 - cystatin C - 1994 2.1.1.72 site-specific DNA-methyltransferase (adenine-specific) 0.0000000046 - methylated DNA - 21984 3.4.17.1 carboxypeptidase A 0.0000000048 - (2S)-2-([hydroxy[(1R)-1-[[1-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)-L-prolyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59952 3.4.22.38 cathepsin K 0.0000000048 - N-[(1S)-3-methyl-1-([(4S)-3-oxo-1-[(3-pyridin-2-ylphenyl)acetyl]azepan-4-yl]carbamoyl)butyl]-5-(2-morpholin-4-ylethoxy)-1-benzofuran-2-carboxamide - 23125 3.5.4.31 S-methyl-5'-thioadenosine deaminase 0.0000000048 - 5'-methylthiocoformycin at pH 7.4 and 25°C 5418 3.4.22.15 cathepsin L 0.0000000049 - chagasin mutant T31S - 26682 3.4.22.38 cathepsin K 0.0000000049 - L-kininogen - 11007 2.4.2.1 purine-nucleoside phosphorylase 0.000000005 - SerMe-immucillin-H pH and temperature not specified in the publication 155401 3.2.2.16 methylthioadenosine nucleosidase 0.000000005 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene) -immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201204 3.4.21.6 coagulation factor Xa 0.000000005 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid (3-fluoro-2'-sulfamoyl-biphenyl-4-yl)-amide - 53189 3.4.21.6 coagulation factor Xa 0.000000005 - 2-(3-carbamimidoyl-phenyl)-5-trifluoromethyl-2H-pyrazole-3-carboxylic acid (3-fluoro-2'-sulfamoyl-biphenyl-4-yl)-amide below 53194 3.4.21.6 coagulation factor Xa 0.000000005 - 2-(3-carbamimidoyl-phenyl)-5-trifluoromethyl-2H-pyrazole-3-carboxylic acid (3-fluoro-2'-methanesulfonyl-biphenyl-4-yl)-amide below 53195 3.4.21.6 coagulation factor Xa 0.000000005 - 3-chloro-N-[4-chloro-2-[(5-chloropyridin-2-yl)carbamoyl]-6-methoxyphenyl]-4-[[2-(methylamino)-1H-imidazol-1-yl]methyl]thiophene-2-carboxamide pH and temperature not specified in the publication 85911 3.4.22.65 peptidase 1 (mite) 0.000000005 - cystatin A pH 8.3 7539 3.4.23.16 HIV-1 retropepsin 0.000000005 - lopinavir wild-type enzyme 1430 3.4.23.16 HIV-1 retropepsin 0.000000005 - lopinavir wild-type protein 1430 3.4.23.16 HIV-1 retropepsin 0.000000005 - darunavir L10I/G48V/I54V/L63P/V82A mutant protein 1901 3.4.23.16 HIV-1 retropepsin 0.000000005 - (3aR,7aR)-octahydro-1-benzofuran-4-yl [(2S,3R)-3-hydroxy-4-{[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino}-1-phenylbutan-2-yl]carbamate pH and temperature not specified in the publication 41897 3.4.23.16 HIV-1 retropepsin 0.0000000052 - (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-yl [(2S,3S)-4-[(4R)-5,5-dimethyl-4-[(2-methylbenzyl)carbamoyl]-1,3-thiazolidin-3-yl]-3-hydroxy-4-oxo-1-phenylbutan-2-yl]carbamate GRL-0355 40209 3.4.22.38 cathepsin K 0.0000000054 - H-kininogen - 16359 3.2.2.16 methylthioadenosine nucleosidase 0.0000000055 - 5'-4-chloro-phenylthio-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201202 3.4.23.16 HIV-1 retropepsin 0.0000000055 - (4R)-N-tert-butyl-3-[(2S,3S)-3-([(2,6-dimethylphenoxy)acetyl]amino)-2-hydroxy-4-phenylbutanoyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxamide - 65950 3.4.22.15 cathepsin L 0.00000000552 - p41-fragment-human wild-type, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at least 10-fold higher than enzyme concentration) 18965 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000006 - (1S)-5-(4-chlorophenylthio)-1-(9-deaza-adenin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 35799 3.4.23.16 HIV-1 retropepsin 0.000000006 - (5S)-3-(3-acetylphenyl)-N-[(2S,3R)-4-[(1,3-benzodioxol-5-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild-type protein 14964 3.4.23.16 HIV-1 retropepsin 0.000000006 - (5S)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-3-[3-(trifluoromethyl)phenyl]-1,3-oxazolidine-5-carboxamide wild-type protein 14966 1.5.1.3 dihydrofolate reductase 0.0000000061 - methotrexate - 241 3.4.21.75 Furin 0.0000000062 - D-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239673 3.4.22.15 cathepsin L 0.0000000068 - chagasin mutant T31Y - 26683 1.5.1.3 dihydrofolate reductase 0.000000007 - methotrexate - 241 3.4.21.6 coagulation factor Xa 0.000000007 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid [5-(2-sulfamoyl-phenyl)-pyridin-2-yl]-amide - 54188 3.4.21.6 coagulation factor Xa 0.000000007 - 3-chloro-N-(4-chloro-2-([(5-chloro-2-pyridinyl)amino]carbonyl)-6-methoxyphenyl)-4-([(4,5-dihydro-2-oxazolyl)methylamino]methyl)-2-thiophenecarboxamide - 54204 3.4.22.15 cathepsin L 0.0000000072 - fragment p41 of major histocompatibility complex class II-associated invariant chain pH 6.0, 25°C 14674 3.4.22.43 cathepsin V 0.0000000072 - fragment p41 of major histocompatibility complex class II-associated invariant chain pH 6.0, 25°C 14674 3.4.22.15 cathepsin L 0.00000000722 - p41-fragment-mouse substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at least 10-fold higher than enzyme concentration) 75370 3.4.22.15 cathepsin L 0.0000000073 - chagasin - 7723 3.4.23.16 HIV-1 retropepsin 0.0000000075 - (hydroxyethyl)amide isostere - 58735 3.4.22.51 cruzipain 0.0000000076 - chagasin - 7723 2.4.2.1 purine-nucleoside phosphorylase 0.0000000077 - 5'-deoxy-immucillin-H 25°C, pH 7.4, mutant enzyme H257G 18223 1.5.1.3 dihydrofolate reductase 0.0000000078 - aminopterin - 1003 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000008 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(4-methylphenylthio)-D-ribitol 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 35800 3.4.21.6 coagulation factor Xa 0.000000008 - 2-(3-carbamimidoyl-phenyl)-5-trifluoromethyl-2H-pyrazole-3-carboxylic acid (2'-methanesulfonyl-biphenyl-4-yl)-amide - 53196 3.4.21.6 coagulation factor Xa 0.000000008 - 1-(3-[amino(imino)methyl]phenyl)-N-[2'-(methylsulfonyl)-[1,1'-biphenyl]-4-yl]-3-methyl-1H-pyrazole-5-carboxamide - 54185 3.4.23.16 HIV-1 retropepsin 0.000000008 - darunavir wild-type enzyme 1901 3.4.23.16 HIV-1 retropepsin 0.000000008 - darunavir wild-type protein 1901 4.1.1.23 orotidine-5'-phosphate decarboxylase 0.000000008 - 6-hydroxyuridine 5'-phosphate wild-type enzyme 11357 3.4.17.1 carboxypeptidase A 0.0000000084 - (2S)-2-([hydroxy[(1R)-1-[[N-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)-L-alanyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59948 3.4.17.21 Glutamate carboxypeptidase II 0.0000000086 - (S)-2-(3-((S)-5-(N-(4-bromobenzyl)-6-(4-(4-(4-(4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl)piperazin-1-yl)phenyl)-piperazin-1-yl)nicotinamido)-1-carboxypentyl)ureido)pentanedioic acid pH and temperature not specified in the publication 239469 3.4.22.1 cathepsin B 0.0000000086 - Z-Arg-Leu-Phe-Agly-Val-Ala-OMe - 60871 3.4.22.38 cathepsin K 0.0000000087 - cystatin C Y67L/L205A mutant cathepsin K 1994 4.1.1.23 orotidine-5'-phosphate decarboxylase 0.0000000088 - 6-hydroxyuridine 5'-phosphate - 11357 1.5.1.3 dihydrofolate reductase 0.000000009 - methotrexate recombinant enzyme 241 1.5.1.3 dihydrofolate reductase 0.000000009 - methotrexate isozyme type III, R-plasmid encoded 241 2.4.2.1 purine-nucleoside phosphorylase 0.000000009 - DADMe-immucillin-H pH and temperature not specified in the publication 27051 2.4.2.1 purine-nucleoside phosphorylase 0.000000009 - DATMe-immucillin-H pH and temperature not specified in the publication 155400 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000009 - (+/-)-trans-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28424 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000009 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(3-methylphenylthio)-D-ribitol 25°C, pH 7.0,dissociation constant for the equilibrium complex of enzyme-inhibitor following slow-onset inhibition 35801 3.4.21.6 coagulation factor Xa 0.000000009 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid (3-chloro-2'-sulfamoyl-biphenyl-4-yl)-amide - 53191 3.4.21.6 coagulation factor Xa 0.000000009 - 2-(3-carbamimidoyl-phenyl)-5-trifluoromethyl-2H-pyrazole-3-carboxylic acid [5-(2-sulfamoyl-phenyl)-pyridin-2-yl]-amide - 53192 3.4.23.16 HIV-1 retropepsin 0.000000009 - atazanavir D30N/L63P/N88D mutant protein 2379 4.1.1.23 orotidine-5'-phosphate decarboxylase 0.000000009 - barbiturate ribonucleoside 5'-monophosphate in 50 mM Tris (pH 7.5), 20 mM dithiothreitol, and 40 mM NaCl, at 22°C 12163 3.4.21.98 hepacivirin 0.0000000091 - cyclopropanesulfonic acid [(Z)-(1R,4R,6S,15R,17R)-17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-13-methyl-2,14-dioxo-3,13-diaza-tricyclo[13.3.0.04,6]octadec-7-ene-4-carbonyl]-amide - 71616 3.2.2.16 methylthioadenosine nucleosidase 0.0000000093 - 5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201203 3.4.22.15 cathepsin L 0.0000000097 - chagasin mutant T31V - 26681 3.4.22.38 cathepsin K 0.0000000099 - N-[(2S)-1-[[(4S,5S)-4,5-dimethyl-3-oxo-1-(pyridin-2-ylsulfonyl)azepan-4-yl]amino]-4-methyl-1-oxopentan-2-yl]-1-benzofuran-2-carboxamide pH and temperature not specified in the publication 81209 1.1.1.205 IMP dehydrogenase 0.00000001 - mizoribine 5'-phosphate pH 8.0, 25°C, recombinant mutant A251T 17995 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.00000001 - 5'-(4-chlorophenyl)thio-DADMe-immucillin A pH and temperature not specified in the publication 252853 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.00000001 - 5'-methylthio-immucillin A pH and temperature not specified in the publication 252854 3.4.17.21 Glutamate carboxypeptidase II 0.00000001 - N-([(1S)-1-carboxy-5-([(4-iodophenyl)carbonyl]amino)pentyl]carbamoyl)-L-glutamic acid pH not specified in the publication, temperature not specified in the publication 199303 3.4.21.6 coagulation factor Xa 0.00000001 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid (3-bromo-2'-sulfamoyl-biphenyl-4-yl)-amide - 53190 3.4.21.6 coagulation factor Xa 0.00000001 - 2-(3-carbamimidoyl-phenyl)-5-trifluoromethyl-2H-pyrazole-3-carboxylic acid [5-(2-sulfamoyl-phenyl)-pyrimidin-2-yl]-amide - 53193 3.4.22.2 papain 0.00000001 - cystatin SN below 22575 3.4.22.2 papain 0.00000001 - cystatin SN variant G12A/G13A below 54343 3.4.22.2 papain 0.00000001 - cystatin SN variant P106G/W107G below 54344 3.4.22.27 cathepsin S 0.00000001 - cystatin C mutant L9W/W106G pH 7.0, 37°C 115514 3.4.22.36 caspase-1 0.00000001 - cowpox serpin CrmA pH 7.5, 25°C 4359 3.4.23.16 HIV-1 retropepsin 0.00000001 - darunavir - 1901 3.4.23.16 HIV-1 retropepsin 0.00000001 - (3aS,4S,7aR)-hexahydro-4H-furo[2,3-b]pyran-4-yl [(2S,3R)-4-{[(4-aminophenyl)sulfonyl](2-methylpropyl)amino}-3-hydroxy-1-phenylbutan-2-yl]carbamate pH and temperature not specified in the publication 41900 3.4.23.20 Penicillopepsin 0.00000001 - pepstatin pH 5.0, 37°C, wild-type Pencillopepsin JT-2 396 3.4.23.38 plasmepsin I 0.00000001 - 17-Benzyl-14-[2-(3,3-dimethyl-butyrylamino)-3-methyl-butyrylamino]-18-hydroxy-2-isopropyl-3,8,15,20-tetraoxo-1,4,9,16tetraaza-cycloicosane-5-carboxylic acid carbamoylmethyl-amide - 33270 6.1.1.10 methionine-tRNA ligase 0.00000001 - REP8839 wild-type 3414 3.4.22.15 cathepsin L 0.0000000101 - fragment p41 of major histocompatibility complex class II-associated invariant chain pH 6.0, 25°C 14674 3.4.22.15 cathepsin L 0.0000000101 - p41-fragment-human G139R mutant, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at least 10-fold higher than enzyme concentration) 18965 2.4.2.1 purine-nucleoside phosphorylase 0.0000000107 - 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase 6920 2.4.2.1 purine-nucleoside phosphorylase 0.000000011 - immucillin-H 25°C, pH 7.4, mutant enzyme H257G 2113 2.4.2.1 purine-nucleoside phosphorylase 0.000000011 - 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H overall dissociation constant 6920 3.4.21.34 plasma kallikrein 0.000000011 - ecotin ecotin with mutation H53P in the 50s loop, mutations S82W/T83N/M84R/M85R/A86S in the 80s loop and mutations R108S/N110S in the 100s loop, at room temperature 4614 3.4.21.109 matriptase 0.000000011 - L-arginyl-N1-[(2S)-1-[[(2S)-1-(1,3-benzothiazol-2-yl)-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]-L-glutamamide matriptase, pH and temperature not specified in the publication 200424 3.4.22.27 cathepsin S 0.000000011 - cystatin C mutant V10F/W106G pH 7.0, 37°C 115515 3.4.22.51 cruzipain 0.000000011 - chagasin mutant T31S - 26682 3.4.22.51 cruzipain 0.000000011 - chagasin mutant T32E - 75458 3.4.23.16 HIV-1 retropepsin 0.000000011 - (2S,2'S)-N,N'-[(2S,3R,4R,5S)-3,4-dihydroxy-1,6-diphenylhexane-2,5-diyl]bis(3-methyl-2-[[methyl(pyridin-2-ylmethyl)carbamoyl]amino]butanamide) - 24830 4.6.1.18 pancreatic ribonuclease 0.000000011 - oligo(vinylsulfonic acid) without NaCl 35448 3.4.23.38 plasmepsin I 0.0000000117 - pepstatin A pH 2.8, mature fusion protein 309 3.4.21.75 Furin 0.0000000119 - Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239674 3.4.23.16 HIV-1 retropepsin 0.000000012 - (2S,2'S)-N,N'-[(2S,3S,4S,5S)-3,4-dihydroxy-1,6-diphenylhexane-2,5-diyl]bis(3-methyl-2-[[methyl(pyridin-2-ylmethyl)carbamoyl]amino]butanamide) - 65111 3.4.21.109 matriptase 0.0000000123 - scFv antibody E2 - 67277 3.4.22.38 cathepsin K 0.0000000124 - Stefin B wild-type cathepsin K 12910 3.2.2.16 methylthioadenosine nucleosidase 0.0000000125 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 3417 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000013 - trans-4-cyclopentyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 88572 3.4.21.6 coagulation factor Xa 0.000000013 - 1-(3-[amino(imino)methyl]phenyl)-N-[2'-(aminosulfonyl)-[1,1'-biphenyl]-4-yl]-3-methyl-1H-pyrazole-5-carboxamide - 33747 3.4.21.6 coagulation factor Xa 0.000000013 - 1-[3-(aminomethyl)phenyl]-N-[3-fluoro-(2'-(methylsulfonyl))-[1,1'-biphenyl]-4-yl]-3-(trifluoromethyl)-1H-pyrazole-5-carboxamide - 119009 3.4.21.6 coagulation factor Xa 0.000000013 - 1-(3-carbamimidoylphenyl)-3-methyl-N-(2'-sulfamoylbiphenyl-4-yl)-1H-pyrazole-5-carboxamide pH and temperature not specified in the publication 85779 3.4.22.38 cathepsin K 0.0000000135 - L-kininogen Y67L/L205A mutant cathepsin K 11007 3.4.21.75 Furin 0.0000000138 - Nalpha(carbamidoyl)Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239662 1.5.1.3 dihydrofolate reductase 0.000000014 - methotrexate pH 7.4, 37°C, wild-type enzyme 241 3.4.21.4 trypsin 0.000000014 - Putranjiva roxburghii trypsin inhibitor in 50 mM Tris-HCl buffer, pH 8.0 containing 20 mM CaCl2 and 0.001 N HCl, at 30°C 75359 3.4.21.34 plasma kallikrein 0.000000014 - ecotin ecotin with mutation H53P in the 50s loop and mutations S82W/T83N/M84R/M85R/A86S in the 80s loop, at room temperature 4614 3.4.21.37 leukocyte elastase 0.000000014 - GW447631 pH 7.4, 30°C 52580 3.4.22.15 cathepsin L 0.000000014 - chagasin mutant T32A - 26684 3.4.23.16 HIV-1 retropepsin 0.000000014 - N2-acetyl-N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)(pentyl)amino]-1-benzyl-2-hydroxypropyl]-L-valinamide - 65962 3.4.23.16 HIV-1 retropepsin 0.000000014 - tipranavir L10I/G48V/I54V/L63P/V82A mutant protein 2316 4.1.1.1 pyruvate decarboxylase 0.000000014 - 3-deazathiamine diphosphate below 37048 3.4.22.38 cathepsin K 0.0000000145 - H-kininogen Y67L/L205A mutant cathepsin K 16359 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000015 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(3-methylphenylthio)-D-ribitol 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 35801 3.4.17.1 carboxypeptidase A 0.000000015 - (S)-2-(Hydroxy-[(S)-1-[(S)-3-methyl-2-(2-1,4,7,10tetraaza-cyclododec-1-yl-acetylamino)-pentanoylamino]-ethyl]-phosphinoyloxy)-3-phenyl-propionic acid pH 7.5, 25°C 23916 3.4.21.6 coagulation factor Xa 0.000000015 - 1-(3-[amino(imino)methyl]phenyl)-N-[2'-(aminosulfonyl)-[1,1'-biphenyl]-4-yl]-3-(trifluoromethyl)-1H-pyrazole-5-carboxamide trifluoro acetic acid salt - 54190 3.4.21.20 cathepsin G 0.000000015 - ecotin - 4614 3.4.21.26 prolyl oligopeptidase 0.000000015 - 4-phenylbutanoyl-5(R)-tert-butyl-L-Pro-2(S)-(hydroxyacetyl)-pyrrolidine pH 7.0, 30°C 33732 3.4.21.26 prolyl oligopeptidase 0.000000015 - 4-phenylbutanoyl-5(R)-tert-butyl-L-prolyl-2(S)-(hydroxyacetyl)pyrrolidine at 25°C between pH 5.5 and 9.5 201558 3.4.23.B14 plasmepsin IV 0.000000015 - pepstatin A 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 309 3.4.23.16 HIV-1 retropepsin 0.000000015 - ritonavir pH 4.7, wild-type enzyme 996 3.4.23.16 HIV-1 retropepsin 0.000000015 - (5S)-3-(3-acetylphenyl)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41883 1.5.1.3 dihydrofolate reductase 0.000000016 - methotrexate recombinant enzyme derived from E. coli 241 2.4.2.1 purine-nucleoside phosphorylase 0.000000016 - 7-[[(3R,4R)-4-hydroxy-3-(hydroxymethyl)pyrrolidin-2-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one - 76729 3.4.21.6 coagulation factor Xa 0.000000016 - 6-chloro-N-[1-[2-fluoro-4-(2-oxopyridin-1(2H)-yl)phenyl]-2-oxopiperidin-3-yl]thieno[2,3-b]pyridine-2-sulfonamide - 67517 3.4.21.6 coagulation factor Xa 0.000000016 - apixaban 25°C 3564 3.4.22.51 cruzipain 0.000000016 - chagasin mutant T32A - 26684 3.4.22.51 cruzipain 0.000000016 - chagasin mutant T32Y - 26686 3.4.23.16 HIV-1 retropepsin 0.000000016 - lopinavir - 1430 3.4.23.16 HIV-1 retropepsin 0.000000016 - (5S)-N-[(2S,3R)-4-[(1,3-benzodioxol-5-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-3-[3-(trifluoromethyl)phenyl]-1,3-oxazolidine-5-carboxamide wild-type protein 14962 3.4.23.16 HIV-1 retropepsin 0.000000016 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-3-[3-(trifluoromethyl)phenyl]-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41876 3.4.21.37 leukocyte elastase 0.0000000168 - SSR69071 pH 7.8 9677 3.4.17.21 Glutamate carboxypeptidase II 0.000000017 - N-[[(1S)-1-carboxy-5-(4-iodobenzamido)pentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235011 3.4.21.62 Subtilisin 0.000000017 - chymotrypsin inhibitor 2 mutant M59A - 60801 3.4.21.62 Subtilisin 0.000000017 - chymotrypsin inhibitor 2 mutant M59F - 60804 6.1.1.10 methionine-tRNA ligase 0.000000017 - REP3123 - 3044 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000018 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(4-methylphenylthio)-D-ribitol 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 35800 3.4.14.5 dipeptidyl-peptidase IV 0.000000018 - H-(S)-valinyl-(R)-boro-proline in 0.1 M HEPES, 0.14 M NaCl, pH 8.0, at 25°C 26658 3.4.17.21 Glutamate carboxypeptidase II 0.000000018 - N-[[(1S)-1-carboxy-5-([5-[3-(3-[[[2-[[[5-(2-carboxyethyl)-2-hydroxyphenyl]methyl](carboxymethyl)amino]ethyl](carboxymethyl)amino]methyl]-4-hydroxyphenyl)propanamido]pentanoyl]amino)pentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235013 3.4.17.21 Glutamate carboxypeptidase II 0.000000018 - DKFZ-PSMA-11 pH and temperature not specified in the publication 239472 3.4.21.26 prolyl oligopeptidase 0.000000018 - 4-phenylbutanoyl-L-Pro-2(S)-(hydroxyacetyl)-pyrrolidine pH 7.0, 30°C 33731 3.4.23.16 HIV-1 retropepsin 0.000000018 - lopinavir pH 4.7, wild-type enzyme 1430 1.5.1.3 dihydrofolate reductase 0.000000019 - methotrexate pH 7.4, 37°C, mutant Q35K/N64F 241 3.4.21.62 Subtilisin 0.000000019 - chymotrypsin inhibitor 2 mutant M59K - 60802 3.4.23.16 HIV-1 retropepsin 0.000000019 - tipranavir - 2316 3.4.23.B14 plasmepsin IV 0.0000000197 - pepstatin A 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 309 2.4.2.1 purine-nucleoside phosphorylase 0.00000002 - 5'-deoxy-immucillin-H 25°C, pH 7.4, wild-type enzyme 18223 3.3.2.10 soluble epoxide hydrolase 0.00000002 - 1-[4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)phenyl]-3-[1-(2-methylbutanoyl)piperidin-4-yl]urea in sodium phosphate buffer (0.1 M, pH 7.4), at 30°C 200034 3.3.2.10 soluble epoxide hydrolase 0.00000002 - 1-[4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)phenyl]-3-[1-(3,3,3-trifluoropropanoyl)piperidin-4-yl]urea Ki below 0.00000002 mM, in sodium phosphate buffer (0.1 M, pH 7.4), at 30°C 200043 3.4.21.6 coagulation factor Xa 0.00000002 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid (3-fluoro-2'-methanesulfonyl-biphenyl-4-yl)-amide - 54189 3.4.21.6 coagulation factor Xa 0.00000002 - N2-[(6-chloronaphthalen-2-yl)sulfonyl]-N-[2-(dimethylamino)ethyl]-N2-[1-[2-fluoro-4-(2-oxopyridin-1(2H)-yl)phenyl]-2-oxopiperidin-3-yl]-N-methylglycinamide - 67516 3.4.21.26 prolyl oligopeptidase 0.00000002 - 4-phenylbutanoyl-L-prolyl-2(S)-cyanopyrrolidine at 25°C between pH 5.5 and 9.5 201560 3.4.21.27 coagulation factor XIa 0.00000002 - methyl [(11S)-11-([(2E)-3-[5-chloro-2-(1H-tetrazol-1-yl)phenyl]prop-2-enoyl]amino)-6-fluoro-2-oxo-1,3,4,10,11,13-hexahydro-2H-5,9:15,12-di(azeno)-1,13-benzodiazacycloheptadecin-18-yl]carbamate at 37°C, pH not specified in the publication 236473 3.4.21.98 hepacivirin 0.00000002 - (1R,12E,20S,23S)-20-cyclohexyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81021 3.4.21.98 hepacivirin 0.00000002 - (1R,12E,22S,25S)-22-tert-butyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,20,23-trioxo-2,19-dioxa-4,21,24-triazatetracyclo[22.2.1.14,7.06,11]octacosa-6,8,10,12-tetraene-25-carboxamide Ki below 0.00000002 mM, in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81026 3.4.22.15 cathepsin L 0.00000002 - chagasin mutant T32V - 26687 3.4.22.51 cruzipain 0.00000002 - chagasin mutant P30A - 26679 3.4.22.51 cruzipain 0.00000002 - chagasin mutant T32S - 26685 3.4.22.51 cruzipain 0.00000002 - chagasin mutant Y89F - 75457 3.4.23.16 HIV-1 retropepsin 0.00000002 - tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2]-Phe-Gln-Phe-NH2 wild-type enzyme 35417 3.4.23.16 HIV-1 retropepsin 0.00000002 - (5S)-3-(3-aminophenyl)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41889 4.1.1.1 pyruvate decarboxylase 0.00000002 - 2-[1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-methyl-1H-1,2,3-triazol-4-yl]ethyl trihydrogen diphosphate - 65396 4.1.1.1 pyruvate decarboxylase 0.00000002 - 2-[1-(4-amino-2-methylpyrimidin-5-ylmethyl)-5-methyl-1H-[1,2,3]triazol-4-yl]ethyl diphosphate - 71799 6.1.1.10 methionine-tRNA ligase 0.00000002 - REP3123 wild-type 3044 3.4.17.21 Glutamate carboxypeptidase II 0.000000021 - N-[[(1S)-5-([(4-bromophenyl)methyl][6-[4-(4-[4-[4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl]piperazin-1-yl]phenyl)piperazin-1-yl]pyridine-3-carbony]amino)-1-carboxypentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235014 3.4.21.6 coagulation factor Xa 0.000000021 - 1-(4-methoxyphenyl)-6-[4-[1-(pyrrolidin-1-ylmethyl)cyclopropyl]phenyl]-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 72881 3.4.23.16 HIV-1 retropepsin 0.000000021 - AG1776 pH 4.7, 37°C, recombinant LAI wild-type enzyme 7656 1.3.1.9 enoyl-[acyl-carrier-protein] reductase (NADH) 0.000000022 - 2-(o-tolyloxy)-5-hexylphenol pH 6.8, temperature not specified in the publication 41134 3.4.21.26 prolyl oligopeptidase 0.000000022 - 4-phenylbutanoyl-5(R)-t-butyl-L-prolyl-2(S)-(hydroxyacetyl)pyrrolidine pH 7.0, 23°C 59671 2.4.2.1 purine-nucleoside phosphorylase 0.000000023 - DADMe-immucillin-H pH and temperature not specified in the publication 27051 3.4.21.26 prolyl oligopeptidase 0.000000023 - 4-phenylbutanoyl-L-prolyl-2(S)cyanopyrrolidine pH 7.0, 23°C 59669 3.4.21.26 prolyl oligopeptidase 0.000000023 - KYP-2047 pH and temperature not specified in the publication 167767 3.4.21.36 pancreatic elastase 0.000000024 - OMTKY3 pH 8.5, 25 C 17262 3.4.21.36 pancreatic elastase 0.000000024 - OMTKY3 pH 8.5, 25°C 17262 3.4.23.39 plasmepsin II 0.000000024 - pepstatin A - 309 3.4.21.34 plasma kallikrein 0.000000025 - ecotin ecotin with mutations S82W/T83N/M84R/M85R/A86S in the 80s loop and mutations R108S/N110S in the 100s loop, at room temperature 4614 3.4.21.35 tissue kallikrein 0.000000025 - Protein C inhibitor pH 7.5, 37°C 3251 3.4.22.51 cruzipain 0.000000025 - chagasin mutant Y89S - 75456 3.4.23.16 HIV-1 retropepsin 0.000000025 - darunavir L10I/L63P/A71V/G73S/I84V/L90M mutant protein 1901 3.4.23.16 HIV-1 retropepsin 0.000000025 - (5S)-N-[(2S,3R)-4-[(1,3-benzodioxol-5-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-3-[3-(methylsulfonyl)phenyl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41885 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000026 - (3R,4S)-4-(4-chlorophenyl-thiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 35802 3.4.17.1 carboxypeptidase A 0.000000026 - N-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)glycyl-N-[(1R)-1-[[(1S)-1-carboxy-2-phenylethoxy](hydroxy)phosphoryl]ethyl]glycinamide pH 7.5, 25°C 59946 3.4.21.26 prolyl oligopeptidase 0.000000026 - 4-phenylbutanoyl-5(R)-methyl-L-Pro-2(S)-(hydroxyacetyl)pyrrolidine pH 7.0, 30°C 33733 3.4.23.B14 plasmepsin IV 0.000000026 - L-lysyl-L-prolyl-L-phenylalanyl-N-[(1S)-1-benzyl-2-[[(1S)-1-benzyl-2-[[(1S)-2-[[(1S)-1-carboxy-2-hydroxyethyl]amino]-1-(hydroxymethyl)-2-oxoethyl]amino]-2-oxoethyl]amino]ethyl]-L-norleucinamide - 24107 3.4.23.B14 plasmepsin IV 0.000000026 - Lys-Pro-Phe-Nle-Phe-Phe-Ser-Ser 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 10154 3.4.23.16 HIV-1 retropepsin 0.000000026 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-3-[3-(trifluoromethoxy)phenyl]-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41881 3.4.14.5 dipeptidyl-peptidase IV 0.000000027 - H-(S)-alaninyl-(R)-boro-proline in 0.1 M HEPES, 0.14 M NaCl, pH 8.0, at 25°C 26659 3.4.21.1 chymotrypsin 0.000000027 - IVNGEEAVPGSWPW - 60772 3.4.23.16 HIV-1 retropepsin 0.000000027 - N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-1-benzyl-2-hydroxypropyl]-3-hydroxybenzamide - 65859 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000028 - (3R,4S)-4-(benzylthiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 8658 3.4.17.21 Glutamate carboxypeptidase II 0.000000028 - N-[[(1S)-1-carboxy-5-(4-iodobenzamido)pentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235011 3.4.21.75 Furin 0.0000000283 - N2-phenylacetyl-Arg-Ala-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239666 1.5.1.3 dihydrofolate reductase 0.0000000289 - methotrexate 25°C 241 1.5.1.3 dihydrofolate reductase 0.000000029 - methotrexate pH 7.4, 37°C, mutant N64F 241 1.3.1.9 enoyl-[acyl-carrier-protein] reductase (NADH) 0.00000003 - triclosan 25°C, pH 7.4 744 1.5.1.3 dihydrofolate reductase 0.00000003 - 3-(2-(3-[(2,4-diamino-6-ethylpyrimidin-5-yl)oxy]propoxy)phenyl)propanoic acid pH 6, temperature not specified in the publication, isolate PKNY146CSP 262470 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.00000003 - tert-butyl trans-3-hydroxy-4-vinylpyrrolidine-1-carboxylate pH 7.4, 25°C 88573 3.4.14.5 dipeptidyl-peptidase IV 0.00000003 - (2S)-1-[(2R)-2-(dihydroxyboranyl)pyrrolidin-1-yl]-1-oxopropan-2-aminium - 24675 3.4.21.6 coagulation factor Xa 0.00000003 - BMS-740808 - 64778 3.4.21.6 coagulation factor Xa 0.00000003 - 1-(3-amino-1,2-benzoxazol-5-yl)-6-(2'-[[(3R)-3-hydroxypyrrolidin-1-yl]methyl]biphenyl-4-yl)-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one pH and temperature not specified in the publication 85840 3.4.21.37 leukocyte elastase 0.00000003 - trappin-2 neutrophil elastase 13874 3.4.21.61 Kexin 0.00000003 - eglin c mutant Tyr replaced with Glu at P4 pH 7.5, 37°C 54160 3.4.21.98 hepacivirin 0.00000003 - (1R,12E,21S,24S)-21-tert-butyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,19,22-trioxo-2,18-dioxa-4,20,23-triazatetracyclo[21.2.1.14,7.06,11]heptacosa-6,8,10,12-tetraene-24-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81025 3.4.23.24 Candidapepsin 0.00000003 - benzyloxycarbonyl-L-Val-L-Val-statine-L-Ala-statine-OH isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C 27586 3.4.24.B4 matrix metalloproteinase-13 0.00000003 - N-hydroxy-2,2-dimethyl-4-[[4-(pyridin-4-yloxy)phenyl]sulfonyl]thiomorpholine-3-carboxamide pH not specified in the publication, temperature not specified in the publication 82152 4.1.1.1 pyruvate decarboxylase 0.00000003 - 2-[1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-1H-1,2,3-triazol-4-yl]ethyl trihydrogen diphosphate - 65395 4.1.1.1 pyruvate decarboxylase 0.00000003 - 2-[1-(4-amino-2-methylpyrimidin-5-ylmethyl)-1H-[1,2,3]triazol-4-yl]ethyl diphosphate - 71798 1.5.1.3 dihydrofolate reductase 0.000000031 - methotrexate wild-type, pH 7.6, 23°C 241 3.4.22.51 cruzipain 0.000000031 - chagasin mutant W93A - 26678 3.4.23.46 memapsin 2 0.000000031 - N3-[(1S,2R)-1-benzyl-2-hydroxy-3-[[(1S,2S)-2-hydroxy-1-(isobutylcarbamoyl)pentyl]amino]propyl]-N1-methyl-N1-[(1R)-1-phenylpropyl]benzene-1,3-dicarboxamide pH and temperature not specified in the publication 239952 2.4.2.1 purine-nucleoside phosphorylase 0.000000032 - (3S,4S)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol overall dissociation constant 69038 3.4.22.15 cathepsin L 0.000000032 - chagasin mutant T32S - 26685 3.4.23.16 HIV-1 retropepsin 0.000000032 - tipranavir L10I/L63P/A71V/G73S/I84V/L90M mutant protein 2316 3.4.23.16 HIV-1 retropepsin 0.000000033 - N2-acetyl-N-[(1S,2R)-1-benzyl-2-hydroxy-3-[[(4-methoxyphenyl)sulfonyl][(2S)-2-methylbutyl]amino]propyl]-L-valinamide - 65960 3.4.23.16 HIV-1 retropepsin 0.000000033 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-3-phenyl-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41859 3.4.21.75 Furin 0.0000000337 - N2-phenylacetyl-Arg-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239665 3.4.21.75 Furin 0.0000000337 - Arg-Arg-Arg-Val-Arg-4-amidinobenzylamide pH and temperature not specified in the publication 235209 3.4.21.62 Subtilisin 0.0000000339 - fungal protease inhibitor F mutant T29M 10107 2.4.2.1 purine-nucleoside phosphorylase 0.000000034 - DADMe-immucillin-G wild-tpe, pH 7.4, 25°C 39772 3.4.21.6 coagulation factor Xa 0.000000034 - therostasin - 54161 2.4.2.44 S-methyl-5'-thioinosine phosphorylase 0.000000035 - 5'-phenylthio-immucillin-H pH 7.4, 25°C 40336 3.4.21.6 coagulation factor Xa 0.000000035 - 6-(4-[1-[(dimethylamino)methyl]cyclopropyl]phenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 38682 3.4.23.16 HIV-1 retropepsin 0.000000035 - (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-yl-(2S,3R)-3-hydroxy-4-(4-methoxy-N-(((S)-2-oxooxazolidin-4-yl)methyl)phenylsulfonamido)-1-phenylbutan-2-ylcarbamate - 79017 3.4.22.2 papain 0.000000036 - chagasin inactivation of papain is very fast, rate constant for inactivation is 1500 mM/s 7723 3.4.23.16 HIV-1 retropepsin 0.000000036 - N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-1-benzyl-2-hydroxypropyl]-3-hydroxybenzamide - 65865 3.4.21.34 plasma kallikrein 0.000000037 - ecotin ecotin with mutations S82W/T83N/M84R/M85R/A86S in the 80s loop, at room temperature 4614 3.4.23.39 plasmepsin II 0.000000037 - pepstatin pH 5.0, 37°C 396 3.4.21.75 Furin 0.0000000378 - D-Arg-D-Arg-D-Arg-D-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239672 3.2.2.16 methylthioadenosine nucleosidase 0.000000038 - 5'-4-chloro-phenylthio-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201202 3.4.21.6 coagulation factor Xa 0.000000038 - 6-[4-(1-[[(3R)-3-hydroxypyrrolidin-1-yl]methyl]cyclopropyl)phenyl]-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 73447 3.4.21.61 Kexin 0.000000038 - eglin variant M2 RVKR pH 7.5, 37°C 22542 3.4.23.16 HIV-1 retropepsin 0.000000038 - N2-acetyl-N-[(1S,2R)-1-benzyl-2-hydroxy-3-[[(4-methoxyphenyl)sulfonyl](pentyl)amino]propyl]-L-valinamide - 65959 3.4.23.16 HIV-1 retropepsin 0.000000039 - N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-3-hydroxybenzamide wild-type protein 14958 3.4.23.16 HIV-1 retropepsin 0.000000039 - (5S)-3-(3-acetylphenyl)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide D30N/L63P/N88D mutant protein 14963 3.4.11.18 methionyl aminopeptidase 0.00000004 - 5-(benzylsulfanyl)-N-(2-methylphenyl)-4H-1,2,4-triazol-3-amine apparent value 63692 3.4.11.18 methionyl aminopeptidase 0.00000004 - N-[5-(benzylsulfanyl)-4H-1,2,4-triazol-3-yl]pyridin-3-amine apparent value 63794 3.4.21.5 thrombin 0.00000004 - DV24K10RY pH and temperature not specified in the publication, after no preincubation time 10350 3.4.21.6 coagulation factor Xa 0.00000004 - 1-(3-[amino(imino)methyl]phenyl)-N-[2'-(trifluoromethyl)-[1,1'-biphenyl]-4-yl]-3-methyl-1H-pyrazole-5-carboxamide - 54186 3.4.21.6 coagulation factor Xa 0.00000004 - 1-(3-amino-1,2-benzisoxazol-5-yl)-6-[2'-[(dimethylamino)methyl]biphenyl-4-yl]-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 67524 3.4.21.98 hepacivirin 0.00000004 - (1R,12E,20S,23S)-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]-carbonyl]-2-vinylcyclopropyl)-20-isopropyl-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81020 3.4.21.98 hepacivirin 0.00000004 - (1R,12E,20S,23S)-20-cyclopentyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81023 3.4.21.98 hepacivirin 0.00000004 - (1R,12E,20S,23S)-20-tert-butyl-N-((1R,2R)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-ethylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81028 3.4.22.38 cathepsin K 0.00000004 - relacatib pH and temperature not specified in the publication 3945 3.4.23.16 HIV-1 retropepsin 0.00000004 - AG1776 pH 4.7, 37°C, recombinant V6 wild-type enzyme 7656 3.4.23.16 HIV-1 retropepsin 0.00000004 - saquinavir pH 4.7, wild-type enzyme 997 3.4.23.16 HIV-1 retropepsin 0.00000004 - lopinavir D30N/L63P/N88D mutant protein 1430 3.4.23.16 HIV-1 retropepsin 0.00000004 - darunavir mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C 1901 3.4.23.16 HIV-1 retropepsin 0.00000004 - darunavir wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C 1901 3.4.23.39 plasmepsin II 0.00000004 - (5S,8S,12S,13S,16S,19S)-13-benzyl-12-hydroxy-8,23,23-trimethyl-16,19-bis(1-methylethyl)-5-(2-methylpropyl)-4,7,10,15,18,21-hexaoxo-3,6,9,14,17,20-hexaazatetracosan-1-amide - 65236 4.2.1.84 nitrile hydratase 0.00000004 - phenylboronic acid pH 7.5, 25°C 2577 6.1.1.10 methionine-tRNA ligase 0.00000004 - REP8839 mutant L213W 3414 3.4.21.6 coagulation factor Xa 0.000000041 - 2-(3-carbamimidoyl-phenyl)-5-methyl-2H-pyrazole-3-carboxylic acid [5-(2-sulfamoyl-phenyl)-pyrimidin-2-yl]-amide - 53188 3.4.22.38 cathepsin K 0.000000041 - relicatib apparent value, pH and temperature not specified in the publication 81211 3.4.22.38 cathepsin K 0.000000041 - relacatib pH and temperature not specified in the publication 3945 3.4.22.38 cathepsin K 0.000000041 - N-[(2S)-4-methyl-1-[[(4S,7R)-7-methyl-3-oxo-1-(pyridine-2-sulfonyl)azepan-4-yl]amino]-1-oxopentan-2-yl]-2,3-dihydro-1-benzofuran-2-carboxamide pH and temperature not specified in the publication 236831 3.4.23.16 HIV-1 retropepsin 0.000000041 - (4R)-3-[(2S,3S)-2-hydroxy-3-[[(3-hydroxy-2-methylphenyl)carbonyl]amino]-4-phenylbutanoyl]-5,5-dimethyl-N-(2-methylbenzyl)-1,3-thiazolidine-4-carboxamide - 23887 3.4.23.16 HIV-1 retropepsin 0.000000041 - darunavir D30N/L63P/N88D mutant protein 1901 1.5.1.3 dihydrofolate reductase 0.000000042 - methotrexate pH 7.4, 37°C, wild-type enzyme 241 3.4.21.6 coagulation factor Xa 0.000000042 - 1-(4-methoxyphenyl)-6-[4-(1-[[(1-methylethyl)amino]methyl]cyclopropyl)phenyl]-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 72879 3.4.24.83 anthrax lethal factor endopeptidase 0.000000042 - (2S)-6-[(1R)-N-1-(4-fluorophenyl)propan]aminoamino-2-(4-fluoro-3,5-dimethylbenzyl)-N-hydroxyhexanamide pH not specified in the publication, temperature not specified in the publication 204538 3.2.1.20 alpha-glucosidase 0.000000043 - de-O-sulfonated ponkoranol versus the C-terminal subunit, pH and temperature not specified in the publication 15214 3.4.21.6 coagulation factor Xa 0.000000043 - methyl N-[(6-chlorothieno[2,3-b]pyridin-2-yl)sulfonyl]-N-[2-oxo-1-[4-(2-oxopyridin-1(2H)-yl)phenyl]piperidin-3-yl]glycinate - 67523 3.4.21.98 hepacivirin 0.000000043 - (1aR,5S,8S,9S,10R,22aR)-5-tert-butyl-N-[(1R,2R)-1-[(cyclopropanesulfonyl)carbamoyl]-2-(difluoromethyl)cyclopropyl]-9-ethyl-18,18-difluoro-14-methoxy-3,6-dioxo-1,1a,3,4,5,6,9,10,18,19,20,21,22,22a-tetradecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235346 3.4.22.15 cathepsin L 0.000000045 - chagasin mutant P30A - 26679 3.4.23.1 pepsin A 0.0000000457 - pepstatin A at 25°C, pH not specified in the publication 309 3.4.21.75 Furin 0.0000000459 - N2-(5-(guanidino)valeroyl)-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239663 3.4.23.16 HIV-1 retropepsin 0.000000046 - atazanavir wild-type enzyme 2379 3.4.23.16 HIV-1 retropepsin 0.000000046 - atazanavir wild-type protein 2379 3.4.23.16 HIV-1 retropepsin 0.000000046 - N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-3-hydroxy-1-phenylbutan-2-yl]-3-hydroxybenzamide wild-type protein 14956 1.14.14.19 steroid 17alpha-monooxygenase 0.0000000464 - transforming growth factor-beta1 - 6762 1.5.1.3 dihydrofolate reductase 0.000000047 - methotrexate pH 7.4, 37°C, mutant Q35S 241 1.5.1.3 dihydrofolate reductase 0.000000047 - methotrexate pH 7.4, 37°C, mutant Q35S/N64S 241 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000047 - (+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-isobutylpyrrolidine pH 7.4, 25°C 28425 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000048 - (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine 25°C, pH 7.0, dissociation constant for the equilibrium between enzyme and inhibitor before slow-onset inhibition 11566 3.2.2.16 methylthioadenosine nucleosidase 0.000000048 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A - 3417 3.4.21.26 prolyl oligopeptidase 0.000000048 - isophthalic acid 2(S)-(cyclopentanecarbonyl)pyrrolidine-L-prolyl-2(S)-(hydroxyacetyl)pyrrolidine amide pH 7.0, 23°C 59667 3.4.17.21 Glutamate carboxypeptidase II 0.000000049 - N-[[(1S)-5-([(4-bromophenyl)methyl][6-[4-(4-[4-[4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl]piperazin-1-yl]phenyl)piperazin-1-yl]pyridine-3-carbony]amino)-1-carboxypentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235014 3.4.17.21 Glutamate carboxypeptidase II 0.000000049 - (S)-2-(3-((S)-5-(6-(4-(4-(4-acetylpiperazin-1-yl)phenyl)piperazin-1-yl)-N-(4-bromobenzyl)nicotinamido)-1-carboxypentyl)ureido)-pentanedioic acid pH and temperature not specified in the publication 239467 3.4.22.16 cathepsin H 0.000000049 - chicken cystatin pH 6.5, 25°C, mutant lacking the mini chain 4082 3.4.23.16 HIV-1 retropepsin 0.000000049 - (5S)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-3-[3-(methylsulfonyl)phenyl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41884 3.4.21.75 Furin 0.0000000491 - N2-phenylacetyl-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239664 3.4.21.109 matriptase 0.0000000497 - Bovine pancreatic trypsin inhibitor - 2774 1.5.1.3 dihydrofolate reductase 0.00000005 - pyrimethamine pH 6, temperature not specified in the publication, isolate PKNY146CSP 761 3.4.11.18 methionyl aminopeptidase 0.00000005 - 5-(benzylsulfanyl)-N-(4-methoxyphenyl)-4H-1,2,4-triazol-3-amine apparent value 63695 3.4.17.21 Glutamate carboxypeptidase II 0.00000005 - N-[[(1S)-5-[acetyl[(4-bromophenyl)methyl]amino]-1-carboxypentyl]carbamoyl]-L-glutamic acid 37°C, pH 7.4 235012 3.4.21.6 coagulation factor Xa 0.00000005 - 6-chloro-N-[2-oxo-1-[4-(2-oxopyridin-1(2H)-yl)phenyl]piperidin-3-yl]thieno[2,3-b]pyridine-2-sulfonamide - 67520 3.4.21.98 hepacivirin 0.00000005 - vaniprevir in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 40404 3.4.21.98 hepacivirin 0.00000005 - (1R,12E,20S,23S)-20-tert-butyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81018 3.4.21.98 hepacivirin 0.00000005 - (1R,12E,20S,23S)-20-butyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81019 3.4.21.98 hepacivirin 0.00000005 - (1R,20S,23S)-20-tert-butyl-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-vinylcyclopropyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10-triene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81027 3.4.22.15 cathepsin L 0.00000005 - cystatin A 0.1 M acetate pH 5.5, 1 mM EDTA, 2 mM dithiothreitol, 100 mg/ml bovine serum albumin, 10 min 7539 3.4.22.15 cathepsin L 0.00000005 - cystatin B 0.1 M acetate pH 5.5, 1 mM EDTA, 2 mM dithiothreitol, 100 mg/ml bovine serum albumin, 10 min 9663 3.4.22.27 cathepsin S 0.00000005 - cathepsin S propeptide wild-type enzyme 8495 3.4.22.35 Histolysain 0.00000005 - EhICP2 pH 5.0, 37°C 14261 3.4.23.16 HIV-1 retropepsin 0.00000005 - lopinavir - 1430 3.4.23.16 HIV-1 retropepsin 0.00000005 - atazanavir - 2379 3.4.24.80 membrane-type matrix metalloproteinase-1 0.00000005 - TIMP-2 Tyr112-Gly288 fragment 1110 3.4.21.75 Furin 0.000000051 - N2-phenylacetyl-Ala-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239667 3.4.23.16 HIV-1 retropepsin 0.000000051 - (5S)-3-[3-(acetylamino)phenyl]-N-[(2S,3R)-4-[(1,3-benzodioxol-5-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41892 3.4.22.1 cathepsin B 0.000000052 - Z-Arg-Nle-Val-Agly-Gly-Glu-OMe - 60868 3.4.21.98 hepacivirin 0.000000053 - (1aR,5S,8S,9S,10R,19E,22aR)-5-tert-butyl-N-[(1R,2R)-2-(difluoromethyl)-1-[(1-methylcyclopropane-1-sulfonyl)carbamoyl]cyclopropyl]-9-ethyl-18,18-difluoro-14-methoxy-3,6-dioxo-1,1a,3,4,5,6,9,10,18,21,22,22a-dodecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235344 3.4.21.75 Furin 0.0000000538 - human proteinase inhibitor 8 pH 7.5, 37°C 54248 3.4.21.75 Furin 0.0000000538 - cyclo[succinyl-Phe-2-Nal-Arg-Arg-Arg-Arg-Lys]-Arg 4-amidinobenzylamide pH and temperature not specified in the publication 235219 2.4.2.1 purine-nucleoside phosphorylase 0.000000054 - 5'-deoxy-immucillin-H 25°C, pH 7.4, mutant enzyme H257D 18223 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000054 - (+/-)-trans-4-[3-(benzylthio)propyl]-1-[(9-deazaadenin-9-yl)-methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28433 3.4.21.6 coagulation factor Xa 0.000000054 - 1-(4-methoxyphenyl)-6-(4-[1-[(methylamino)methyl]cyclopropyl]phenyl)-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 72877 3.2.1.20 alpha-glucosidase 0.000000055 - 3'-O-methylponkoranol versus the N-terminal subunit N20, pH and temperature not specified in the publication 12176 3.4.21.6 coagulation factor Xa 0.000000055 - 1-(4-methoxyphenyl)-6-(4-[1-[(methylamino)methyl]cyclopropyl]phenyl)-7-oxo-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide - 72878 3.4.21.26 prolyl oligopeptidase 0.000000055 - benzylcarbamoyl-L-prolyl-2(S)-(hydroxyacetyl)pyrrolidine at 25°C between pH 5.5 and 9.5 201561 3.4.23.16 HIV-1 retropepsin 0.000000055 - ritonavir wild-type enzyme 996 3.4.23.16 HIV-1 retropepsin 0.000000055 - ritonavir wild-type protein 996 6.1.1.10 methionine-tRNA ligase 0.000000055 - REP8839 - 3414 3.4.22.36 caspase-1 0.000000056 - acetyl-WEHD-aldehyde pH 7.5, 25°C 4959 3.4.21.75 Furin 0.0000000563 - N2-phenylacetyl-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239661 3.4.21.6 coagulation factor Xa 0.000000057 - 5-chloro-N-(5-chloropyridin-2-yl)-3-methoxy-2-[[4-(2-oxopiperidin-1-yl)benzoyl]amino]benzamide pH and temperature not specified in the publication 85918 3.4.21.34 plasma kallikrein 0.000000057 - taicatoxin serine protease inhibitor in 0.1 MTris-HCl, pH 7.4, 0.01% (v/v) Tween 80 at 25°C 86363 3.4.23.16 HIV-1 retropepsin 0.000000057 - N2-acetyl-N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-1-benzyl-2-hydroxypropyl]-L-valinamide - 65963 2.4.2.1 purine-nucleoside phosphorylase 0.0000000579 - immucillin-H 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase 2113 2.4.2.1 purine-nucleoside phosphorylase 0.000000058 - immucillin-H pH and temperature not specified in the publication 2113 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000059 - (+/-)-trans-4-(cyclohexylmethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28428 3.2.2.16 methylthioadenosine nucleosidase 0.000000059 - 5'-4-chloro-phenylthio-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 201202 3.4.17.1 carboxypeptidase A 0.000000059 - (2S)-2-([hydroxy[(1R)-1-[[N-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)-L-phenylalanyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59954 2.1.1.45 thymidylate synthase 0.00000006 - D1694 - 13332 2.7.1.20 adenosine kinase 0.00000006 - (2S,3S,4R,5R)-2-amino-5-(4-amino-5-iodo-7H-pyrrolo[2,3-d]pyrimidin-7-yl)tetrahydrofuran-3,4-diol Ki-value determined by on-line capillary electrophoresis 36415 3.2.1.18 exo-alpha-sialidase 0.00000006 - zanamivir - 2693 3.2.1.20 alpha-glucosidase 0.00000006 - 3'-O-methylponkoranol versus the N-terminal subunit N2, pH and temperature not specified in the publication 12176 3.4.21.5 thrombin 0.00000006 - DV24Ysulf pH and temperature not specified in the publication, after no preincubation time 28571 3.4.21.6 coagulation factor Xa 0.00000006 - 5-butyl-2-(3-carbamimidoyl-phenyl)-2H-pyrazole-3-carboxylic acid (2'-sulfamoyl-biphenyl-4-yl)-amide - 53197 3.4.21.98 hepacivirin 0.00000006 - (1R,12E,20S,23S)-N-((1R,2S)-1-[[(cyclopropylsulfonyl)amino]-carbonyl]-2-vinylcyclopropyl)-20-(1-methylcyclohexyl)-3,18,21-trioxo-2,17-dioxa-4,19,22-triazatetracyclo[20.2.1.14,7.06,11]hexacosa-6,8,10,12-tetraene-23-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81022 3.4.21.98 hepacivirin 0.00000006 - MK-7009 NS3/4A genotype 1b 19232 3.4.23.B14 plasmepsin IV 0.00000006 - Lys-Pro-Ile-Nle-Phe-Phe-Arg-Leu 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 10152 3.4.24.80 membrane-type matrix metalloproteinase-1 0.00000006 - TIMP-2 Tyr112-Gly288 fragment 1110 6.1.1.10 methionine-tRNA ligase 0.00000006 - REP8839 mutant A247E 3414 3.4.23.16 HIV-1 retropepsin 0.000000062 - N-[(1S,2R)-1-benzyl-2-hydroxy-3-[[(4-methoxyphenyl)sulfonyl][(2S)-2-methylbutyl]amino]propyl]-4-oxohexanamide - 65854 3.4.23.16 HIV-1 retropepsin 0.000000062 - N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl][(2S)-2-methylbutyl]amino]-1-phenylbutan-2-yl]-4-oxohexanamide wild-type protein 14957 1.5.1.3 dihydrofolate reductase 0.000000063 - methotrexate pH 7.4, 37°C, mutant K37Q 241 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000063 - (+/-)-trans-4-cyclopropyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28427 3.4.21.4 trypsin 0.000000063 - cMCoTI-II pH not specified in the publication, temperature not specified in the publication 80782 3.4.21.98 hepacivirin 0.000000063 - (1aR,5S,8S,9S,10R,22aR)-5-tert-butyl-N-[(1R,2R)-2-(difluoromethyl)-1-[(1-methylcyclopropane-1-sulfonyl)carbamoyl]cyclopropyl]-9-ethyl-18,18-difluoro-14-methoxy-3,6-dioxo-1,1a,3,4,5,6,9,10,18,19,20,21,22,22a-tetradecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235350 3.4.23.16 HIV-1 retropepsin 0.000000063 - (2S)-N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-1-benzyl-2-hydroxypropyl]-2-hydroxy-3-methylbutanamide - 65105 3.4.23.16 HIV-1 retropepsin 0.000000063 - N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-1-benzyl-2-hydroxypropyl]-4-oxohexanamide - 65866 3.4.23.16 HIV-1 retropepsin 0.000000063 - (2S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-3-hydroxy-1-phenylbutan-2-yl]-2-hydroxy-3-methylbutanamide wild-type protein 14971 3.4.23.16 HIV-1 retropepsin 0.000000063 - N2-acetyl-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(pentyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-L-valinamide I50V/A71V mutant protein 14959 3.4.23.16 HIV-1 retropepsin 0.000000063 - (5S)-3-(2,4-difluorophenyl)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41867 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000064 - (+/-)-trans-4-(1-benzyl-1H-1,2,3-triazol-4-yl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine pH 7.4, 25°C 28432 3.4.21.6 coagulation factor Xa 0.000000064 - 1-(4-methoxyphenyl)-6-[4-[1-(morpholin-4-ylmethyl)cyclopropyl]phenyl]-3-(trifluoromethyl)-1,4,5,6-tetrahydro-7H-pyrazolo[3,4-c]pyridin-7-one - 72880 3.4.24.27 thermolysin 0.000000064 - 1-beta-D-arabinofuranosyl-N4-lauroylcytosine 25°C, pH 7.5 39067 3.4.23.16 HIV-1 retropepsin 0.000000065 - saquinavir wild-type enzyme 997 3.4.23.16 HIV-1 retropepsin 0.000000065 - saquinavir wild-type protein 997 2.4.2.1 purine-nucleoside phosphorylase 0.000000066 - cis-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethylpyrrolidin-3-ol overall dissociation constant 69037 3.4.21.34 plasma kallikrein 0.000000066 - ecotin wild-type ecotin, at room temperature 4614 3.4.21.98 hepacivirin 0.000000067 - (1aR,5S,10R,22aR)-5-tert-butyl-N-[(1R,2R)-2-(difluoromethyl)-1-[(1-methylcyclopropane-1-sulfonyl)carbamoyl]cyclopropyl]-9-ethyl-14-methoxy-3,6-dioxo-1,1a,3,4,5,6,9,10,18,19,20,21,22,22a-tetradecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235341 3.4.23.16 HIV-1 retropepsin 0.000000067 - (3R)-N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)[(2S)-2-methylbutyl]amino]-1-benzyl-2-hydroxypropyl]-3-hydroxypentanamide - 65160 3.5.4.31 S-methyl-5'-thioadenosine deaminase 0.000000067 - 5'-propylthio-2'-deoxycoformycin at pH 7.4 and 25°C 206609 3.4.23.16 HIV-1 retropepsin 0.000000068 - (3aR,4R,7aS)-hexahydro-4H-furo[2,3-b]pyran-4-yl [(2S,3R)-3-hydroxy-4-{[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino}-1-phenylbutan-2-yl]carbamate pH and temperature not specified in the publication 41896 3.4.24.27 thermolysin 0.000000068 - carbobenzoxy-L-phenylalanine-phosphonamidate-L-leucyl-L-alanine pH and temperature not specified in the publication 206310 3.4.24.79 pappalysin-1 0.000000068 - stanniocalcin-1 isoform PAPP-A, at pH 7.5 and 37°C 240071 3.4.23.38 plasmepsin I 0.0000000699 - pepstatin A pH 5.0, mature fusion protein 309 3.2.2.9 adenosylhomocysteine nucleosidase 0.00000007 - ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl 6937 3.2.2.9 adenosylhomocysteine nucleosidase 0.00000007 - 5'-ethylthio-DADMe-ImmucillinA in 50 mM phosphate buffer, pH 7.9, 10 mM KCl, at 25°C 14861 3.2.2.16 methylthioadenosine nucleosidase 0.00000007 - ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl 6937 3.4.11.18 methionyl aminopeptidase 0.00000007 - 5-(benzylsulfanyl)-N-(4-methylphenyl)-4H-1,2,4-triazol-3-amine apparent value 63696 3.4.21.34 plasma kallikrein 0.00000007 - human IgG1 monoclonal antibody DX-2930 at pH 7.5 and 30°C 201594 3.4.21.35 tissue kallikrein 0.00000007 - diisopropyl fluorophosphate pH 7.2, 25°C 244 3.4.21.98 hepacivirin 0.00000007 - [(Z)-(1R,4R,6S,15R,17R)-4-cyclopropanesulfonylaminocarbonyl-17-(7-methoxy-2-phenyl-quinolin-4-yloxy)-2,14-dioxo-3,13-diaza-tricyclo[13.3.0.04,6]octadec-7-en-13-yl]-carbamic acid tert-butyl ester - 71613 3.4.21.98 hepacivirin 0.00000007 - MK-7009 NS3/4A genotype 1a 19232 3.4.22.51 cruzipain 0.00000007 - chagasin mutant T31V - 26681 3.4.23.16 HIV-1 retropepsin 0.00000007 - (R)-N-(2,6-dimethylbenzyl)-3-[(2S,3S)-2-hydroxy-3-(3-amino-2-chlorobenzoyl)amino-4-(3,4-methylenedioxyphenyl)butanoyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxamide - 66593 3.4.23.16 HIV-1 retropepsin 0.00000007 - ritonavir HIV-1 subtype B protease 996 3.4.23.16 HIV-1 retropepsin 0.00000007 - atazanavir HIV-1 subtype B protease 2379 3.4.23.16 HIV-1 retropepsin 0.00000007 - nelfinavir pH 4.7, wild-type enzyme 1193 3.4.23.16 HIV-1 retropepsin 0.00000007 - saquinavir mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C 997 3.4.23.39 plasmepsin II 0.00000007 - pepstatin A at pH 5.0 and 25°C 309 3.4.21.109 matriptase 0.0000000704 - scFv antibody S4 - 67278 3.4.17.21 Glutamate carboxypeptidase II 0.000000071 - (S)-2-(3-((S)-5-(6-(4-(4-(4-acetylpiperazin-1-yl)phenyl)piperazin-1-yl)nicotinamido)-1-carboxypentyl)ureido)pentanedioic acid pH and temperature not specified in the publication 239466 3.4.22.16 cathepsin H 0.000000071 - cystatin C, full-length 25°C, pH 7.0 54335 2.4.2.1 purine-nucleoside phosphorylase 0.000000072 - immucillin-H - 2113 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000073 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl 3417 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000073 - 5'-methylthio-DADMe-ImmucillinA in 50 mM phosphate buffer, pH 7.9, 10 mM KCl, at 25°C 10251 3.2.2.16 methylthioadenosine nucleosidase 0.000000073 - methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl 3417 3.4.22.38 cathepsin K 0.000000073 - N-(1-([(cyanomethyl)amino]carbonyl)cyclohexyl)-4-[2-(4-methylpiperazin-1-yl)-1,3-thiazol-4-yl]benzamide recombinant cathepsin K 18971 3.4.23.16 HIV-1 retropepsin 0.000000073 - (5S)-3-(4-acetylphenyl)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41873 3.4.21.6 coagulation factor Xa 0.000000074 - 1-(4-methoxyphenyl)-7-oxo-6-[4-[1-(pyrrolidin-1-ylmethyl)cyclopropyl]phenyl]-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide - 72883 3.4.22.16 cathepsin H 0.000000074 - human stefin B pH 6.5, 25°C, mutant lacking the mini chain 33168 3.4.21.37 leukocyte elastase 0.000000075 - Eglin c pH 7.3, 37°C 2653 3.4.21.75 Furin 0.0000000754 - Arg-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239668 2.4.2.44 S-methyl-5'-thioinosine phosphorylase 0.000000076 - 5'-methylthio-immucillin-H pH 7.4, 25°C 7644 3.4.17.1 carboxypeptidase A 0.000000077 - (2S)-2-([hydroxy[(1S)-1-[[1-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)-L-prolyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59951 3.4.21.75 Furin 0.0000000786 - N2-acetyl-D-Leu-Leu-Leu-Leu-Arg-Val-Lys 4-amidinobenzylamide pH 7.0, 22°C 239679 3.4.21.26 prolyl oligopeptidase 0.000000079 - 2-hydroxy-1-[(2S)-1-[1-[(3-[[(2S)-2-(pyrrolidin-1-ylcarbonyl)pyrrolidin-1-yl]carbonyl]phenyl)carbonyl]-D-prolyl]pyrrolidin-2-yl]ethanone pH 7.0, 23°C 59665 3.4.21.91 Flavivirin 0.000000079 - Aprotinin enzyme from Dengue virus serotype 1 405 1.5.3.14 polyamine oxidase (propane-1,3-diamine-forming) 0.00000008 - 1-[7-[(9-carbamimidamidononyl)amino]heptyl]-3-(2-cyclopropylethyl)guanidine pH 6.5, 25°C 76217 3.2.1.169 protein O-GlcNAcase 0.00000008 - thiamet G truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication 232781 3.2.2.9 adenosylhomocysteine nucleosidase 0.00000008 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-methylthio-D-ribitol pH and temperature not specified in the publication 57006 3.2.2.16 methylthioadenosine nucleosidase 0.00000008 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-methylthio-D-ribitol pH and temperature not specified in the publication 57006 3.2.2.30 aminodeoxyfutalosine nucleosidase 0.00000008 - (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-methylthio-D-ribitol pH and temperature not specified in the publication 57006 3.4.21.4 trypsin 0.00000008 - cyclic SFTI pH not specified in the publication, temperature not specified in the publication 80779 3.4.21.6 coagulation factor Xa 0.00000008 - apixaban - 3564 3.4.21.6 coagulation factor Xa 0.00000008 - apixaban 25°C 3564 3.4.21.6 coagulation factor Xa 0.00000008 - 1-(4-methoxyphenyl)-7-oxo-6-[4-(2-oxopiperidin-1-yl)phenyl]-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide pH and temperature not specified in the publication 85842 3.4.21.37 leukocyte elastase 0.00000008 - elafin neutrophil elastase 2165 3.4.21.98 hepacivirin 0.00000008 - (1R,21S,24S)-N-((1R,2R)-1-[[(cyclopropylsulfonyl)amino]-carbonyl]-2-ethylcyclopropyl)-16,16-dimethyl-21-(1-methylcyclohexyl)-3,19,22-trioxo-2,18-dioxa-4,20,23-triazatetracyclo[21.2.1.14,7.06,11]heptacosa-6,8,10-triene-24-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81036 3.4.21.109 matriptase 0.00000008 - 3-[(2S)-3-[4-(2-aminoethyl)piperidin-1-yl]-2-([[3-(6-amino-2,3,4,5-tetrahydropyridin-3-yl)phenyl]sulfonyl]amino)-3-oxopropyl]benzenecarboximidamide - 26130 3.4.22.15 cathepsin L 0.00000008 - cystatin C 0.1 M acetate pH 5.5, 1 mM EDTA, 2 mM dithiothreitol, 100 mg/ml bovine serum albumin, 10 min 1994 3.4.22.38 cathepsin K 0.00000008 - N-(cyanomethyl)-4-methyl-2-[3'-(2-piperazin-1-yl-1,3-thiazol-4-yl)biphenyl-3-yl]pentanamide - 36899 3.4.22.43 cathepsin V 0.00000008 - clitocypin pH and temperature not specified in the publication 81227 3.4.23.B14 plasmepsin IV 0.00000008 - Lys-Pro-Phe-Nle-Phe-Phe-Ser-Ser 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 10154 3.4.23.16 HIV-1 retropepsin 0.00000008 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-3-(3-fluorophenyl)-2-oxo-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41874 3.5.4.31 S-methyl-5'-thioadenosine deaminase 0.00000008 - 5'-methylthio-2'-deoxycoformycin at pH 7.4 and 25°C 69269 6.1.1.10 methionine-tRNA ligase 0.00000008 - REP3123 MetRS1 3044 6.1.1.10 methionine-tRNA ligase 0.00000008 - REP8839 mutant I57N 3414 3.4.21.98 hepacivirin 0.000000081 - (1aR,5S,8S,9S,10R,22aR)-5-tert-butyl-N-[(1R,2S)-1-[(cyclopropanesulfonyl)carbamoyl]-2-ethenylcyclopropyl]-9-ethyl-18,18-difluoro-14-methoxy-3,6-dioxo-1,1a,3,4,5,6,9,10,18,19,20,21,22,22a-tetradecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235355 3.4.17.1 carboxypeptidase A 0.000000082 - (2S)-2-([hydroxy[(1R)-1-[[N-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)glycyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59944 3.4.23.B14 plasmepsin IV 0.000000083 - Lys-Pro-Ile-Nle-Phe-Phe-Arg-Leu 37°C, pH 4.4, substrate: KPIEFF(NO2)RL 10152 3.4.23.16 HIV-1 retropepsin 0.000000084 - N2-acetyl-N-[(1S,2R)-1-benzyl-2-hydroxy-3-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]propyl]-L-valinamide - 65958 3.4.23.16 HIV-1 retropepsin 0.000000085 - (3aS,4S,7aR)-hexahydro-4H-furo[2,3-b]pyran-4-yl {(2S,3R)-3-hydroxy-4-[{[4-(hydroxymethyl)phenyl]sulfonyl}(2-methylpropyl)amino]-1-phenylbutan-2-yl}carbamate pH and temperature not specified in the publication 41901 2.4.2.1 purine-nucleoside phosphorylase 0.000000086 - immucillin-H 25°C, pH 7.4, mutant enzyme H257D 2113 2.4.2.28 S-methyl-5'-thioadenosine phosphorylase 0.000000086 - 5'-methylthio-DADMe-immucillin A pH and temperature not specified in the publication 42313 3.4.23.16 HIV-1 retropepsin 0.000000086 - (5S)-N-[(2S,3R)-3-hydroxy-4-[[(4-methoxyphenyl)sulfonyl](2-methylpropyl)amino]-1-phenylbutan-2-yl]-2-oxo-3-[2-(trifluoromethyl)phenyl]-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41863 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000087 - 5'-hexylthio-DADMe-immucillin A pH and temperature not specified in the publication 253257 3.4.21.7 plasmin 0.000000087 - DX-1000 pH and temperature not specified in the publication 201714 3.4.21.109 matriptase 0.000000088 - N1-[(2S)-1-[[(2S)-1-(1,3-benzothiazol-2-yl)-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]-L-glutamamide matriptase, pH and temperature not specified in the publication 200427 3.4.23.16 HIV-1 retropepsin 0.000000088 - tipranavir wild-type enzyme 2316 3.4.23.16 HIV-1 retropepsin 0.000000088 - tipranavir wild-type protein 2316 3.2.2.16 methylthioadenosine nucleosidase 0.000000089 - methylthio-immucillin-A in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 10054 2.7.1.20 adenosine kinase 0.00000009 - (2S,3S,4R,5R)-2-amino-5-(4-amino-5-iodo-7H-pyrrolo[2,3-d]pyrimidin-7-yl)tetrahydrofuran-3,4-diol Ki-value determined by off-line capillary electrophoresis 36415 3.4.17.1 carboxypeptidase A 0.00000009 - (2S)-2-([hydroxy[(1S)-1-[[N-(1,4,7,10-tetraazacyclododecan-1-ylacetyl)-L-alanyl]amino]ethyl]phosphoryl]oxy)-3-phenylpropanoic acid pH 7.5, 25°C 59947 3.4.21.6 coagulation factor Xa 0.00000009 - tissue factor pathway inhibitor recombinant mutant R150A, 22°C 7395 3.4.21.98 hepacivirin 0.00000009 - (1'R,21'S,24'S)-21'-tert-butyl-N-((1R,2R)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-ethylcyclopropyl)-3',19',22'-trioxo-2',18'-dioxa-4',20',23'-triazaspiro[cyclobutane-1,16'-tetracyclo-[21.2.1.14,7.06,11]heptacosane]-6',8',10'-triene-24'-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81032 3.4.21.98 hepacivirin 0.00000009 - (1'R,21'S,24'S)-21'-tert-butyl-N-((1R,2R)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-ethylcyclopropyl)-3',19',22'-trioxo-2',18'-dioxa-4',20',23'-triazaspiro[cyclopentane-1,16'-tetracyclo-[21.2.1.14,7.06,11]heptacosane]-6',8',10'-triene-24'-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81033 3.4.21.98 hepacivirin 0.00000009 - (1R,21S,24S)-21-cyclohexyl-N-((1R,2R)-1-[[(cyclopropylsulfonyl)amino]carbonyl]-2-ethylcyclopropyl)-16,16-dimethyl-3,19,22-trioxo-2,18-dioxa-4,20,23-triazatetracyclo[21.2.1.14,7.06,11]-heptacosa-6,8,10-triene-24-carboxamide in 50 mM HEPES, pH 7.5, 150 mM NaCl, 15% (v/v) glycerol, 0.15% (w/v) Triton X-100, 10 mM dithiothreitol, and 0.1% (w/v) PEG 8000 81035 3.4.23.B14 plasmepsin IV 0.00000009 - N-[(4-[4-[(1-[[(1S,2S)-4-(butylamino)-2-hydroxy-1-(2-methylpropyl)-4-oxobutyl]carbamoyl]-2-methylbutyl)carbamoyl]phenoxy]phenyl)carbonyl]-L-alanyl-N-[4-[(6-methoxyquinolin-8-yl)amino]pentyl]-L-lysinamide 37°C, pH 4.4 10132 1.5.1.3 dihydrofolate reductase 0.000000093 - methotrexate pH 7.4, 37°C, wild-type enzyme 241 2.4.2.1 purine-nucleoside phosphorylase 0.000000093 - 5'-thio-immucillin-H pH 7.4, temperature not specified in the publication 209520 3.4.21.98 hepacivirin 0.000000094 - (1aR,5S,8S,9S,10R,22aR)-5-tert-butyl-N-[(1R,2S)-1-[(cyclopropanesulfonyl)carbamoyl]-2-ethenylcyclopropyl]-18,18-difluoro-14-methoxy-9-methyl-3,6-dioxo-1,1a,3,4,5,6,9,10,18,19,20,21,22,22a-tetradecahydro-8H-7,10-methanocyclopropa[18,19][1,10,3,6]dioxadiazacyclononadecino[11,12-b]quinoxaline-8-carboxamide pH and temperature not specified in the publication 235354 3.4.23.16 HIV-1 retropepsin 0.000000094 - N2-acetyl-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(pentyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-L-valinamide D30N/L63P/N88D mutant protein 14959 3.4.21.75 Furin 0.0000000943 - D-Arg-Arg-Arg-Val-Arg 4-amidinobenzylamide pH 7.0, 22°C 239669 1.5.1.3 dihydrofolate reductase 0.000000095 - methotrexate pH 7.4, 25°C, recombinant enzyme 241 2.1.1.45 thymidylate synthase 0.000000095 - methotrexate pH 7.4, 25°C, recombinant enzyme 241 3.4.22.16 cathepsin H 0.000000095 - chicken egg white cystatin mutant T11_E18del-cathepsin H, pH 6.8, 30°C 17269 3.4.21.59 Tryptase 0.0000000953 - 6-amidino-2-naphthyl p-guanidinobenzoate dimethane sulfonate pH 7.8, 37°C 54158 3.2.1.20 alpha-glucosidase 0.000000096 - de-O-sulfonated ponkoranol versus the N-terminal subunit N20, pH and temperature not specified in the publication 15214 3.4.21.34 plasma kallikrein 0.000000097 - ecotin ecotin with mutation H53P in the 50s loop, at room temperature 4614 3.4.23.16 HIV-1 retropepsin 0.000000097 - (5S)-N-[(2S,3R)-4-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-3-hydroxy-1-phenylbutan-2-yl]-2-oxo-3-[2-(trifluoromethyl)phenyl]-1,3-oxazolidine-5-carboxamide wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C 41865 3.4.23.16 HIV-1 retropepsin 0.000000098 - ritonavir - 996 3.2.2.9 adenosylhomocysteine nucleosidase 0.000000099 - 5'-(2-ethylhex-1-yl)thio-DADMe-immucillin A pH and temperature not specified in the publication 253258 3.4.22.16 cathepsin H 0.000000099 - human stefin A pH 6.5, 25°C, mutant lacking the mini chain 33167 3.4.23.16 HIV-1 retropepsin 0.000000099 - N2-acetyl-N-[(1S,2R)-3-[(1,3-benzothiazol-6-ylsulfonyl)(2-methylpropyl)amino]-1-benzyl-2-hydroxypropyl]-L-valinamide - 65961 3.4.23.16 HIV-1 retropepsin 0.000000099 - (3aS,5R,6aR)-hexahydro-2H-cyclopenta[b]furan-5-yl-(2S,3R)-3-hydroxy-4-(4-methoxy-N-(((R)-5-oxopyrrolidin-2-yl)methyl)phenylsulfonamido)-1-phenylbutan-2-ylcarbamate GRL-02031 79079