1.3.99.31	FAD	added to the reaction mixture	710999, 711020	
1.3.99.31	FAD	CRTI utilizes FAD as the sole redox-active cofactor, binding kinetics and structure analysis, molecular docking, overview. Oxygen, replaceable by quinones in its absence, is needed as the terminal electron acceptor. FAD, besides its catalytic role also displays a structural function by enabling the formation of enzymatically active CRTI membrane associates	726300	
1.3.99.31	FAD	involved in desaturation. FAD is a dissociable moiety and not covalently bound to the enzyme	712337	
1.3.99.31	FAD	required	763447	
1.3.99.31	additional information	no activity with FMN, NAD+, and NADP+	726300