4.2.3.4 additional information no cofactor: NADP+ 729105 4.2.3.4 NAD+ - 137624, 137628, 137629, 137633, 137635, 137636, 137637, 137641, 137643, 5467, 5486, 666031, 690254, 690739, 718819 4.2.3.4 NAD+ 1 mol of NAD+ per mol of protein 137623 4.2.3.4 NAD+ cofactor NAD binds in the cleft region formed between the N- and C-terminal domains of each DHQS monomer, with its nicotinamide ring oriented toward the metal ion 763209 4.2.3.4 NAD+ determination of constants of the cyclic reduction and oxidation of NAD+ and NADH. There is a transient increase in absorbance at 340 nm associated with NADH formation followed by its oxidation. The rates of NAD+ to NADH to NAD+ conversions are not rate limiting because they are larger than the kcat value 716188 4.2.3.4 NAD+ required 729105 4.2.3.4 NAD+ required for activity 718818 4.2.3.4 NADH - 649156, 680499