4.2.1.49 NAD+ - 4.2.1.49 NAD+ full enzyme activity requires NAD+ in both subunits 4.2.1.49 NAD+ tightly enzyme-bound 4.2.1.49 NAD+ tightly bound cofactor 4.2.1.49 NAD+ each monomer of urocanase contains one molecule of tightly bound NAD+, which is required for its activity