1.21.99.5 corrinoid dicyano-cobalamin 349700, 349706 1.21.99.5 corrinoid one mol of dehalogenase contains 1.0 mol of corrinoid 349700 1.21.99.5 corrinoid presence of a corrinoid in the cob(II)alamin form 349700 1.21.99.5 corrinoid vitamin B12 349700, 349707 1.21.99.5 vitamin B12 - 349700, 349701, 349703, 349707 1.21.99.5 menaquinone membrane-bound menaquinone 349702 1.21.99.5 corrinoid Co(I) corrinoid cofactor 349703 1.21.99.5 corrinoid - 349702, 349704, 657587 1.21.99.5 corrinoid contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit 349706 1.21.99.5 corrinoid corrinoid protein 349706 1.21.99.5 corrinoid dicyano-cob(III)alamin 349706 1.21.99.5 corrinoid corrinoid probably noncavalently bound to enzyme 349708 1.21.99.5 corrinoid Co2+ corrinoid 349709 1.21.99.5 additional information no effect by NADH 349709 1.21.99.5 Cobalamin - 657587 1.21.99.5 corrinoid cofactor is absent in strain N and therefore the strain is unable to dechlorinate tetrachloroethene 657689 1.21.99.5 additional information anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12 727374 1.21.99.5 vitamin B12 corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion 727374 1.21.99.5 vitamin B12 PceA dehalogenase harbors either norpseudo-B12 as cofactor when Sulfurospirillum multivorans has been cultivated in the absence - or nor-B12 when Sulfurospirillum multivorans has been cultivated in its presence of 4,6-dimethylbenzimidazole. Dehalogenation rates with norpseudo-B12 reductive dehalogenase are faster than with nor-B12 740384