1.21.99.5 Cobalamin - 657587 1.21.99.5 corrinoid - 349702, 349704, 657587 1.21.99.5 corrinoid Co(I) corrinoid cofactor 349703 1.21.99.5 corrinoid Co2+ corrinoid 349709 1.21.99.5 corrinoid cofactor is absent in strain N and therefore the strain is unable to dechlorinate tetrachloroethene 657689 1.21.99.5 corrinoid contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit 349706 1.21.99.5 corrinoid corrinoid probably noncavalently bound to enzyme 349708 1.21.99.5 corrinoid corrinoid protein 349706 1.21.99.5 corrinoid dicyano-cob(III)alamin 349706 1.21.99.5 corrinoid dicyano-cobalamin 349700, 349706 1.21.99.5 corrinoid one mol of dehalogenase contains 1.0 mol of corrinoid 349700 1.21.99.5 corrinoid presence of a corrinoid in the cob(II)alamin form 349700 1.21.99.5 corrinoid vitamin B12 349700, 349707 1.21.99.5 menaquinone membrane-bound menaquinone 349702 1.21.99.5 additional information anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12 727374 1.21.99.5 additional information no effect by NADH 349709 1.21.99.5 vitamin B12 - 349700, 349701, 349703, 349707 1.21.99.5 vitamin B12 corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion 727374 1.21.99.5 vitamin B12 PceA dehalogenase harbors either norpseudo-B12 as cofactor when Sulfurospirillum multivorans has been cultivated in the absence - or nor-B12 when Sulfurospirillum multivorans has been cultivated in its presence of 4,6-dimethylbenzimidazole. Dehalogenation rates with norpseudo-B12 reductive dehalogenase are faster than with nor-B12 740384