1.21.98.3	Cobalamin	-	
1.21.98.3	S-adenosyl-L-methionine	-	
1.21.98.3	[4Fe-4S]-center	BchE sequence displays the conserved CXXXCXXC sequence essential for 4Fe-4S cluster formation and an active 4Fe-4S cluster is present in the protein. Isolated protein is yellow-brown in colour with an absorption peak around 410 nm	
1.21.98.3	Cobalamin	dependent on, ligated via Asp248, Glu249, Leu29, Thr71, Val97	
1.21.98.3	S-adenosyl-L-methionine	dependent on, ligated via Phe210, Glu308 and Lys320	
1.21.98.3	[4Fe-4S] cluster	ligated viaCys204, Cys208, Cys211	
1.21.98.3	additional information	membrane-localized BchE requires an additional, heat-sensitive cytosolic cobalamin component for activity, BchE catalysis is not sustained in chimeric experiments when a cytosolic extract from Escherichia coli is used as a substitute	
1.21.98.3	Cobalamin	vitamin B12-requiring mutants of the bluE and bluB genes of Rhodobacter capsulatus, grown without B12, accumulate Mg-protoporphyrin monomethyl ester and its 3-vinyl-8-ethyl derivative. Cyclase activity in the B12-dependent mutants requires vitamin B12 but not protein synthesis