1.21.99.5	corrinoid	one mol of dehalogenase contains 1.0 mol of corrinoid	349700	
1.21.99.5	corrinoid	presence of a corrinoid in the cob(II)alamin form	349700	
1.21.99.5	corrinoid	vitamin B12	349700, 349707	
1.21.99.5	menaquinone	membrane-bound menaquinone	349702	
1.21.99.5	additional information	anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12	727374	
1.21.99.5	additional information	no effect by NADH	349709	
1.21.99.5	vitamin B12	-	349700, 349701, 349703, 349707	
1.21.99.5	vitamin B12	corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion	727374	
1.21.99.5	vitamin B12	PceA dehalogenase harbors either norpseudo-B12 as cofactor when Sulfurospirillum multivorans has been cultivated in the absence - or nor-B12 when Sulfurospirillum multivorans has been cultivated in its presence of 4,6-dimethylbenzimidazole. Dehalogenation rates with norpseudo-B12 reductive dehalogenase are faster than with nor-B12	740384