3.5.1.52 2 3.5 remarkable loss of activity above pH 3.5, narrow pH range, profile overview 733540 3.5.1.52 2.5 7.5 - 288913 3.5.1.52 3 5.5 - 288934 3.5.1.52 3 6 enzyme activity declines rapidly above pH 6.0 719759 3.5.1.52 3 6.5 - 288925 3.5.1.52 3 7.5 activity range, profile overview 734113 3.5.1.52 3 8.6 80% of the maximum activity at pH 3.3, 65% of the maximum activity at pH 8.6 288929 3.5.1.52 3.5 4 acid PNGase M, whole embryo, stage 18-28 288933 3.5.1.52 4 7.5 pH 4.0: about 50% of maximal activity, pH 7.5: about 40% of maximal activity, surface-displayed PNGase F 686438 3.5.1.52 4 8.5 - 288909 3.5.1.52 5 10 pH 5: about 50% of maximal activity, pH 10.0: about 70% of maximal activity 684119 3.5.1.52 5.5 7 - 288928 3.5.1.52 6 9 pH 6: about 70% of maximal activity, pH 9.0: about 60% of maximal activity 684908 3.5.1.52 6.5 7 no activity below 5.5 288926 3.5.1.52 6.8 7 - 288922 3.5.1.52 7.5 8.5 neutral PNGase M, middle blastula blastoderm, stage 7-13 288933