3.4.22.53 additional information - the enzyme is not proteolytically active at pH 5.8 3.4.22.53 5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 30% of maximal activity 3.4.22.53 6 8 pH 6.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, enzyme from retina and brain 3.4.22.53 6 8.5 pH 6.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity 3.4.22.53 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity 3.4.22.53 6.5 7.5 activity of m-calpain is greater at pH 7.5 than at pH 6.5