4.1.3.43	additional information	-	additional information	2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH	721615	
4.1.3.43	additional information	-	additional information	value not detectable for R16A variant of BphI	714244	
4.1.3.43	additional information	-	additional information	with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold	721659	
4.1.3.43	0.043	-	(4R)-4-hydroxy-2-oxopentanoate	L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.05	-	oxaloacetate	L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.08	-	4-hydroxy-2-oxopentanoate	with Ca2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.08	-	oxaloacetate	L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.089	-	(4S)-4-hydroxy-2-oxopentanoate	H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.098	-	(4R)-4-hydroxy-2-oxopentanoate	L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.099	-	(4S)-4-hydroxy-2-oxopentanoate	H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244