1.13.11.27 0.014 - 4-hydroxyphenylpyruvate mutant Q293A, pH 7, 25°C 765756 1.13.11.27 0.03 - 4-hydroxyphenylpyruvate pH 7.0, 25°C, mutant N245Q 724325 1.13.11.27 0.039 - 4-hydroxyphenylpyruvate mutant N282A, pH 7, 25°C 765756 1.13.11.27 0.06 - 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C 764109 1.13.11.27 0.06 - 4-hydroxyphenylpyruvate pH 6.5, 37°C, mutant E254D 726347 1.13.11.27 0.065 - 4-hydroxyphenylpyruvate activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C 672170 1.13.11.27 0.07 - 4-hydroxyphenylpyruvate pH 6.5, 37°C, mutant R378K 726347 1.13.11.27 0.09 - 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C 764109 1.13.11.27 0.1 - 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C 764109 1.13.11.27 0.1 - 4-hydroxyphenylpyruvate pH 6.5, 37°C, mutant DELTAG379 726347