6.3.4.2 additional information - additional information - 648997 6.3.4.2 0.0058 - 2',3'-dialdehyde adenosine 5'-triphosphate K306A: replacement of lysine 306 by alanine reduces the rate of 2',3'-dialdehyde adenosine 5'-triphosphate-dependent inactivation 672395 6.3.4.2 0.031 0.51 NH3 mutant K306A, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 0.054 - 2',3'-dialdehyde adenosine 5'-triphosphate in the presence of 10 mM UTP 672395 6.3.4.2 0.063 - Gln-OH 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 0.08 - UTP pH 8.0, 37°C, mutant G142A with L-glutamine, in presence of 0.25 mM GTP 690817 6.3.4.2 0.18 - NH3 pH 8.0, mutant enzyme H118A 648962 6.3.4.2 0.233 - glutamine reaction without GTP 648992 6.3.4.2 0.453 - Gln-OH 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 0.67 - UTP pH 8.0, 37°C, mutant G143A with L-glutamine, in presence of 0.25 mM GTP 690817 6.3.4.2 0.762 - UTP pH 8.0, 65°C 746547 6.3.4.2 0.92 - NH3 pH 8.0, mutant enzyme E103A 648962 6.3.4.2 1.03 - Gln pH 8.0, mutant enzyme L109F 648962 6.3.4.2 1.28 - glutamine mutant K306A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 1.35 - glutamine mutant K306A, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 1.41 - L-glutamine 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 1.49 - Gln 30°C, pH 8.0 660794 6.3.4.2 1.5 - 2'-deoxy-GTP no guanosine 674909 6.3.4.2 1.5 - glutamine wild-type protein, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 1.53 - Gln pH 8.0, mutant enzyme R105A 648962 6.3.4.2 1.64 - Gln pH 8.0, mutant enzyme L109A 648962 6.3.4.2 1.8 - NH4+ 30°C, pH 8.0, mutant enzyme G360P 661713 6.3.4.2 1.86 - Gln 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 1.96 - NH3 pH 8.0, mutant enzymeR104A 648962 6.3.4.2 2.17 - Gln pH 8.0, mutant enzyme G110A 648962 6.3.4.2 2.19 - NH3 mutant K306A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 2.25 - NH3 reaction with or without GTP 648992 6.3.4.2 2.8 - O-methylguanosine 5'-triphosphate no guanosine 674909 6.3.4.2 3.5 - Gln pH 8.0, mutant enzyme D107A 648962 6.3.4.2 4 - guanosine 5'-tetraphosphate no guanosine 674909 6.3.4.2 4.18 - NH3 pH 8.0, mutant enzyme G110A 648962 6.3.4.2 4.2 - UTP pH 8.0, 37°C, mutant G146A with L-glutamine, in presence of 0.25 mM GTP 690817 6.3.4.2 4.22 - Gln pH 8.0, mutant enzyme K102A 648962 6.3.4.2 5 - UTP pH 8.0, 37°C, wild-type enzyme with L-glutamine, in presence of 0.25 mM GTP 690817 6.3.4.2 5.1 - NH4+ 30°C, pH 8.0, mutant enzyme R359P 661713 6.3.4.2 5.2 - ITP no guanosine 674909 6.3.4.2 6.1 - Gln 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 6.1 - Gln pH 8.0, wild-type enzyme 648962 6.3.4.2 6.1 - glutamine wild-type protein, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 6.26 - ATP K306A, presence of 2 mM UTP 672395 6.3.4.2 6.3 - NH4+ 30°C, pH 8.0, wild-type enzyme 661713 6.3.4.2 6.7 - glutamine pH 8.0, wild-type enzyme 648963 6.3.4.2 6.9 - NH4+ 30°C, pH 8.0, mutant enzyme R359M 661713 6.3.4.2 6.9 - UTP K306A, presence of 2 mM ATP 672395 6.3.4.2 7.5 - NH4+ 30°C, pH 8.0, mutant enzyme G360A 661713 6.3.4.2 7.59 - NH3 mutant K306A, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 7.97 - NH3 pH 8.0, mutant enzyme L109A 648962 6.3.4.2 8.1 - glutamine pH 8.0, recombinant His6-tagged enzyme 648963 6.3.4.2 8.2 - GTP 0.2 mM guanosine 674909 6.3.4.2 8.5 - 6-thioguanosine 5'-triphosphate no guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM 674909 6.3.4.2 8.59 - NH3 mutant K297A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 8.7 - NH3 pH 8.0, mutant enzyme D107A 648962 6.3.4.2 8.88 - NH3 pH 8.0, mutant enzyme R105A 648962 6.3.4.2 9.4 - NH3 pH 8.0, wild-type enzyme 648962 6.3.4.2 9.5 - NH3 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 9.5 - NH3 wild-type protein, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 9.9 - NH3 pH 8.0, mutant enzyme C379A 648963 6.3.4.2 10.1 - NH3 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 10.1 - NH4+ 30°C, pH 8.0, mutant enzyme E362Q 661713 6.3.4.2 10.3 - GTP 0.1 mM guanosine 674909 6.3.4.2 10.4 - NH3 pH 8.0, mutant enzyme C379S 648963 6.3.4.2 10.6 - GTP no guanosine 674909 6.3.4.2 10.8 - ATP K297A, presence of 2 mM UTP 672395 6.3.4.2 11 - NH3 pH 8.0, mutant enzyme L109F 648962 6.3.4.2 11.3 - NH3 wild-type protein, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 12.2 - NH3 pH 8.0, mutant enzyme K102A 648962 6.3.4.2 12.8 - ATP wild-type protein, presence of 2 mM UTP 672395 6.3.4.2 13.7 - UTP wild-type protein, presence of 3 mM ATP 672395 6.3.4.2 14 - NH2OH 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 14 - UTP K297A, presence of 3 mM ATP 672395 6.3.4.2 14.1 - NH2OH 37°C, pH 8.0, mutant enzyme L109A 661644