5.3.2.6 0.05 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 0.1 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 0.2 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 0.2 - 2-hydroxymuconate mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.2 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 0.3 - 2-hydroxymuconate mutant 4-OT L8R/I52E, pH 7.3, 23°C 718844 5.3.2.6 0.4 - 2-hydroxy-2,4-hexadienedioate below, pH 7.3, 23°C, mutant R11A/R39A 718830 5.3.2.6 0.4 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 0.6 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 0.6 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 0.6 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 1 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, mutant R11A 718830 5.3.2.6 1.5 - 2-hydroxymuconate mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 2 8 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39A 718830 5.3.2.6 2.4 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 2.5 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 2.6 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 3 6 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 3.4 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 9 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39Q 718830 5.3.2.6 11 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 13 - phenylenolpyruvate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 14 - 2-hydroxymuconate mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 16 - (2E)-2-hydroxy-3-phenylprop-2-enoate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 17 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 18.5 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 32 - 2-hydroxymuconate mutant 4-OT I52E, pH 7.3, 23°C 718844 5.3.2.6 40 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R11A 718830 5.3.2.6 43 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 44 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 61 - 2-hydroxymuconate mutant 4-OT L8R, pH 7.3, 23°C 718844 5.3.2.6 65 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 107 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 360 - 2-hydroxymuconate mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 733 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 1330 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C 747782 5.3.2.6 1850 - 2-hydroxymuconate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 2890 - 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 2940 - 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 3000 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 3500 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 3500 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 3500 - 2-hydroxymuconate wild-type 4-OT, pH 7.3, 23°C 718844 5.3.2.6 4600 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 139000 - 2-hydroxymuconate pH 7.3, 30°C 719633 5.3.2.6 288000 - 2-oxo-3-hexenedioate pH 7.3, 30°C 719633