4.1.3.43	0.043	-	(4R)-4-hydroxy-2-oxopentanoate	L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.098	-	(4R)-4-hydroxy-2-oxopentanoate	L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.131	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244, 722458	
4.1.3.43	0.17	-	(4R)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.089	-	(4S)-4-hydroxy-2-oxopentanoate	H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.099	-	(4S)-4-hydroxy-2-oxopentanoate	H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.132	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244, 722458	
4.1.3.43	0.171	-	(4S)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	4	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	4.07	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.63	-	4-hydroxy-2-oxoheptanoate	pH 8.0, 25°C	721615	
4.1.3.43	0.27	-	4-hydroxy-2-oxohexanoate	G323L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.46	-	4-hydroxy-2-oxohexanoate	G323A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.5	-	4-hydroxy-2-oxohexanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.94	-	4-hydroxy-2-oxohexanoate	G322L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	1.05	-	4-hydroxy-2-oxohexanoate	G322F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	1.1	-	4-hydroxy-2-oxohexanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	1.3	-	4-hydroxy-2-oxohexanoate	G323F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	1.9	-	4-hydroxy-2-oxohexanoate	G322A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	2.1	-	4-hydroxy-2-oxohexanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	2.2	-	4-hydroxy-2-oxohexanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	3.8	-	4-hydroxy-2-oxohexanoate	pH 8.0, 25°C	721615	
4.1.3.43	3.9	-	4-hydroxy-2-oxohexanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	3.9	-	4-hydroxy-2-oxohexanoate	wild-type protein, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.08	-	4-hydroxy-2-oxopentanoate	with Ca2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.1	-	4-hydroxy-2-oxopentanoate	G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.13	-	4-hydroxy-2-oxopentanoate	with Mg2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.286	-	4-hydroxy-2-oxopentanoate	G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.34	-	4-hydroxy-2-oxopentanoate	G323A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.45	-	4-hydroxy-2-oxopentanoate	G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.57	-	4-hydroxy-2-oxopentanoate	with Mn2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.6	-	4-hydroxy-2-oxopentanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.64	-	4-hydroxy-2-oxopentanoate	G323L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.72	-	4-hydroxy-2-oxopentanoate	G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.79	-	4-hydroxy-2-oxopentanoate	wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.79	-	4-hydroxy-2-oxopentanoate	with Ni2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	1	-	4-hydroxy-2-oxopentanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	1	-	4-hydroxy-2-oxopentanoate	with Co2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	1.06	-	4-hydroxy-2-oxopentanoate	G322F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	1.09	-	4-hydroxy-2-oxopentanoate	G322L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	2.3	-	4-hydroxy-2-oxopentanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	2.4	-	4-hydroxy-2-oxopentanoate	G322A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	2.4	-	4-hydroxy-2-oxopentanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	4.07	-	4-hydroxy-2-oxopentanoate	wild-type protein, steady state, pH 8.0, 25°C	721651	
4.1.3.43	4.1	-	4-hydroxy-2-oxopentanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	4.1	-	4-hydroxy-2-oxopentanoate	pH 8.0, 25°C	721615	
4.1.3.43	4.3	-	4-hydroxy-2-oxopentanoate	G323F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.2	-	acetaldehyde	Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.3	-	acetaldehyde	L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.59	-	acetaldehyde	L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.86	-	acetaldehyde	app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.86	-	acetaldehyde	wild-type BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.86	-	acetaldehyde	wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.94	-	acetaldehyde	L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.16	-	Butyraldehyde	Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.26	-	Butyraldehyde	L87A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.3	-	Butyraldehyde	L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.64	-	Butyraldehyde	L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.4	-	glycolaldehyde	app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	additional information	-	additional information	2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH	721615	
4.1.3.43	additional information	-	additional information	value not detectable for R16A variant of BphI	714244	
4.1.3.43	additional information	-	additional information	with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold	721659	
4.1.3.43	0.05	-	oxaloacetate	L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.08	-	oxaloacetate	L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.27	-	oxaloacetate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.4	-	oxaloacetate	L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	16.3	-	oxaloacetate	wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	16.5	-	oxaloacetate	pH 8.0, 25°C	721615	
4.1.3.43	0.13	-	pentaldehyde	L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.14	-	pentaldehyde	Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.58	-	pentaldehyde	L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.1	-	propionaldehyde	L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.14	-	propionaldehyde	L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.16	-	propionaldehyde	Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.29	-	propionaldehyde	L87A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	0.63	-	propionaldehyde	L89A variant of BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	1.79	-	propionaldehyde	app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	1.79	-	propionaldehyde	wild-type BphI, app. kcat-value, pH 8.0, 25°C	714244	
4.1.3.43	1.79	-	propionaldehyde	wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.11	-	pyruvate	L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.16	-	pyruvate	L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.19	-	pyruvate	Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.32	-	pyruvate	L87A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.64	-	pyruvate	L89A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	1.04	-	pyruvate	steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C	702377	
4.1.3.43	1.2	-	pyruvate	wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C	714244, 722458