3.6.1.17 0.00024 - Ap4A mutant enzyme E56Q 656173 3.6.1.17 0.00031 - Ap4A pH 8.0, 25°C, mutant enzyme E59Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 0.005 - Ap4A pH 8.0, 25°C, mutant enzyme E55Q fusion protein containing and N-terminal extension of Gly-Pro-Leu-Gly-Ser 654489 3.6.1.17 0.0052 - Ap4A mutant enzyme E52Q 656173 3.6.1.17 0.011 - Ap4A pH 8.0, 25°C, mutant enzyme E59D, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 0.013 - 5',5''-diadenosine tetraphosphate mutant P133F, pH 7.5, 22°C 733450 3.6.1.17 0.035 - P1,P5-bis(5'-adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ 733872 3.6.1.17 0.042 - 5',5''-diadenosine tetraphosphate wild-type, pH 7.5, 22°C 733450 3.6.1.17 0.055 - P1,P4-bis(5'-adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ 733872 3.6.1.17 0.08 - 5',5''-diadenosine tetraphosphate mutant P133A, pH 7.5, 22°C 733450 3.6.1.17 0.16 - Ap4A mutant enzyme K79M 656173 3.6.1.17 0.2 - P1,P6-bis(5'-adenosyl) hexaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 0.29 - Ap4A pH 8.0, 25°C, mutant enzyme E125Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser 654489 3.6.1.17 0.37 - dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.51 - 2-oxo-dATP in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.53 - dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.58 - 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.6 - Ap4A mutant enzyme K83M 656173 3.6.1.17 0.68 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 0.75 - Ap4A mutant enzyme E103Q 656173 3.6.1.17 1.1 - Ap4A mutant enzyme Y76A/Y121A 656173 3.6.1.17 1.4 - P1,P5-bis(5'-adenosyl) pentaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 1.51 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 1.6 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 1.78 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 2 - Ap4A mutant enzyme Y76A 656173 3.6.1.17 2.1 - Ap4A mutant enzyme K36M 656173 3.6.1.17 2.24 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 2.5 - P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 3.25 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 3.6 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209905 3.6.1.17 3.7 - Ap4A pH 8.0, 25°C, mutant enzyme E58D fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 4 - 5',5'-diadenosine hexaphosphate pH 7.5, 20 mM Mg2+ 687119 3.6.1.17 4.2 - Ap4A pH 8.0, 25°C, mutant enzyme R54Q fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 12 - Ap4A mutant enzyme H31A 656173 3.6.1.17 13 - 5',5'-diadenosine tetraphosphate pH 7.5, 20 mM Mg2+ 687119 3.6.1.17 14 - Ap4A mutant enzyme H31V 656173 3.6.1.17 14 - Ap4A mutant enzyme Y121A 656173 3.6.1.17 17.9 - Ap4A pH 8.0, 25°C, mutant enzyme E58Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 20.4 - Ap4A pH 8.0, 25°C, mutant enzyme E122Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 23 - Ap4A wild-type enzyme 656173 3.6.1.17 26 - Ap4A mutant enzyme Y27A 656173 3.6.1.17 27 - Ap4A pH 7.8, 25°C 654817 3.6.1.17 27 - diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 29 - Ap4A mutant enzyme Y27D 656173 3.6.1.17 30 - Ap4A mutant enzyme K81M 656173 3.6.1.17 31 - Ap4A mutant enzyme H38G 656173 3.6.1.17 31 - Ap4A mutant enzyme H38K 656173 3.6.1.17 40.8 - Ap4A pH 8.0, 25°C, wild-type enzyme 654489 3.6.1.17 43 - 5',5'-diadenosine tetraphosphate pH 6.5, 1 mM Zn2+ 687119