3.4.23.20 additional information - additional information - 30592 3.4.23.20 additional information - additional information effect of chain length of substrates and inhibitors of the turnover number 30597 3.4.23.20 additional information - additional information overview: turnover numbers for the hydrolysis of the peptides of the series Ac-(Ala)m-Lys-(4-nitro)Phe-(Ala)n amide and of the series Ac-Xaa-Lys-(4-nitro)Phe-Ala-Ala amide at the pH optimum for kcat, at pH 5.5 and pH 6.0 30588 3.4.23.20 0.0016 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.0019 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, and mutant T219V 653829 3.4.23.20 0.002 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 and mutant T219A 653829 3.4.23.20 0.003 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.003 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.003 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 0.003 - Ac-Ala-Lys-Ala-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.003 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.004 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.005 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.005 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.0054 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.01 - Ac-Lys 4-nitrophenyl amide pH 5.5 30599 3.4.23.20 0.021 - Ac-Ala-Lys-(4-nitro)Phe amide pH 4.3 (pH-optimum for kcat) 30588 3.4.23.20 0.026 - Ac-Lys-(4-nitro)Phe amide pH 3.8-4.2 (pH-optimum for kcat) 30588 3.4.23.20 0.042 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.046 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.05 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.07 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.09 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.09 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.11 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.12 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.12 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.13 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.14 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.15 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.17 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.23 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.24 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.29 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 0.3 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.3 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.3 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 0.33 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.35 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291V 653829 3.4.23.20 0.35 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.36 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291A 653829 3.4.23.20 0.36 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 0.4 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 0.43 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.45 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291G 653829 3.4.23.20 0.45 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.45 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V and T219G 653829 3.4.23.20 0.5 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.6 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 3.8 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 5 - bovine trypsinogen - 30585 3.4.23.20 6.4 - Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester pH 3.5 30599 3.4.23.20 11.4 - Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester pH 5.5 30599 3.4.23.20 14.9 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 15 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 16.1 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291S 653829 3.4.23.20 28.8 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide pH 3.5 30599 3.4.23.20 34 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 36 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide pH 5.5 30599 3.4.23.20 37.3 - Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu - 30589 3.4.23.20 40 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type pencillopepsin JT-2 653829 3.4.23.20 45 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala-Ala amide pH 5.5 (pH-optimum for kcat) 30588 3.4.23.20 47 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide pH 4.5 (pH-optimum for kcat) 30589 3.4.23.20 420 - Trypsinogen - 668813