1.13.11.50 0.00043 - 4,4-difluoro-1-phenyl-1,3-butanedione apparent value at 25°C 674150 1.13.11.50 0.0015 - 1,1-difluoropentane-2,4-dione mutant Y70F, pH 7.5, 25°C 725553 1.13.11.50 0.0043 - 1,1,1-trifluoro-pentane-2,4-dione native enzyme, using 0.09 mM 1,1,1-trifluoro-pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C 675466 1.13.11.50 0.0049 - 1,1,1-trifluoro-2,4-pentanedione apparent value at 25°C 674150 1.13.11.50 0.007 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A 711267 1.13.11.50 0.008 - 1,1-difluoropentane-2,4-dione mutant F59A, pH 7.5, 25°C 725553 1.13.11.50 0.018 - 1,1-difluoropentane-2,4-dione mutant F115A, pH 7.5, 25°C 725553 1.13.11.50 0.022 - 1,1-difluoropentane-2,4-dione mutant F119A, pH 7.5, 25°C 725553 1.13.11.50 0.033 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A 711267 1.13.11.50 0.036 - 1,1-difluoro-2,4-pentanedione apparent value at 25°C 674150 1.13.11.50 0.036 - 1,1-difluoropentane-2,4-dione wild-type enzyme, pH 7.5, 25°C 725553 1.13.11.50 0.05 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant E98Q 711267 1.13.11.50 0.074 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant E98A 711267 1.13.11.50 0.098 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant R80A/E98A 711267 1.13.11.50 0.1 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant T107A 711267 1.13.11.50 0.126 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant R80A 711267 1.13.11.50 0.13 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70A 711267 1.13.11.50 0.7 - Pentane-2,4-dione mutant F59A, pH 7.5, 25°C 725553 1.13.11.50 1.27 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70F 711267 1.13.11.50 1.27 - Pentane-2,4-dione mutant Y70F, pH 7.5, 25°C 725553 1.13.11.50 1.4 - Pentane-2,4-dione mutant F115A, pH 7.5, 25°C 725553 1.13.11.50 3.2 - Pentane-2,4-dione mutant F119A, pH 7.5, 25°C 725553 1.13.11.50 3.6 - Pentane-2,4-dione mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C 675466 1.13.11.50 6.5 - Pentane-2,4-dione apparent value at 25°C 674150 1.13.11.50 6.6 - 1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant wild-type enzyme 711267 1.13.11.50 6.6 - Pentane-2,4-dione Strep-tagged enzyme 671683 1.13.11.50 6.6 - Pentane-2,4-dione wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C 675466 1.13.11.50 6.6 - Pentane-2,4-dione wild-type enzyme, pH 7.5, 25°C 725553 1.13.11.50 7 - Pentane-2,4-dione recombinant enzyme 671683 1.13.11.50 8.5 - acetylacetate - 657866 1.13.11.50 8.5 - Pentane-2,4-dione native enzyme 671683