1.13.11.20 0.003 - 3-Mercaptopropanoate mutant G95C, PIPES buffer pH 6.5, 37°C 758787 1.13.11.20 0.01 - 3-Mercaptopropanoate pH 6.5, 30°C 741704 1.13.11.20 0.01 - L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme 741704 1.13.11.20 0.011 - 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.1, 37°C 758787 1.13.11.20 0.012 - L-cysteine assay standart conditions are: the enzyme is incubated at 37°C in the presence of 62.5 mM Mes buffer (pH 6.1), 0.3 mM ferrous sulfate, 0.0125 mM bathocuproine disulfonate and 1.2 mM cysteine 676962 1.13.11.20 0.013 - 3-Mercaptopropanoate wild-type, phosphate buffer pH 8.0, 37°C 758787 1.13.11.20 0.019 - 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.5, 37°C 758787 1.13.11.20 0.021 - 3-Mercaptopropanoate pH 7.5, 37°C 759471 1.13.11.20 0.021 - 3-Mercaptopropanoate wild-type, phosphate buffer pH 7.5, 37°C 758787 1.13.11.20 0.022 - 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.5, 37°C 758787 1.13.11.20 0.033 - 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.0, 37°C 758787 1.13.11.20 0.12 - L-cysteine 20% cross-linked wild-type enzyme, pH 9.1, temperature not specified in the publication 741904 1.13.11.20 0.25 - L-cysteine 60% cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication 741904 1.13.11.20 0.25 - L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme 741704 1.13.11.20 0.29 - cysteamine pH 8.0, 25°C 758785 1.13.11.20 0.3 - L-cysteine SCO5772 674292 1.13.11.20 0.33 - L-cysteine not cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication 741904 1.13.11.20 0.33 - L-cysteine SCO3035 674292 1.13.11.20 0.39 - L-cysteine YubC 674292 1.13.11.20 0.62 - L-cysteine - 674292 1.13.11.20 0.82 - 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme 741704 1.13.11.20 1.2 - L-cysteine pH 8.0, 25°C 758785 1.13.11.20 1.7 - L-cysteine determind under saturating oxygen conditions 674873 1.13.11.20 1.8 - L-cysteine presence of 0.1 mol Fe2+, absence of EDTA 676850 1.13.11.20 2 - L-cysteine - 674292 1.13.11.20 3.6 - L-cysteine presence of 0.1 mol Fe2+, absence of EDTA 676850 1.13.11.20 10.35 - L-cysteine pH 6.1, 37°C, recombinnat wild-type enzyme 741585 1.13.11.20 16.9 - N-terminal Cys of RGS5 pH not specified in the publication, 37°C 765832 1.13.11.20 20.1 - N-terminal Cys of RGS4 pH not specified in the publication, 37°C 765832 1.13.11.20 41.2 - O2 cosubstrate N-terminal Cys of RGS5, pH not specified in the publication, 37°C 765832 1.13.11.20 56.6 - O2 cosubstrate N-terminal Cys of RGS4, pH not specified in the publication, 37°C 765832