1.1.1.30 0.00016 - levulinic acid mutant enzyme W187A, at pH 6.5 and 30°C 737914 1.1.1.30 0.0003 - levulinic acid mutant enzyme W187I, at pH 6.5 and 30°C 737914 1.1.1.30 0.0003 - levulinic acid mutant enzyme W187V, at pH 6.5 and 30°C 737914 1.1.1.30 0.00084 - levulinic acid mutant enzyme H144A, at pH 6.5 and 30°C 737914 1.1.1.30 0.00122 - levulinic acid mutant enzyme H144L/W187A, at pH 6.5 and 30°C 737914 1.1.1.30 0.002 - levulinic acid mutant enzyme H144L/W187V, at pH 6.5 and 30°C 737914 1.1.1.30 0.00289 - levulinic acid mutant enzyme H144L/W187I, at pH 6.5 and 30°C 737914 1.1.1.30 0.00465 - levulinic acid mutant enzyme W187L, at pH 6.5 and 30°C 737914 1.1.1.30 0.006 - levulinic acid recombinant wild type enzyme with N-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.00766 - levulinic acid mutant enzyme H144V, at pH 6.5 and 30°C 737914 1.1.1.30 0.00768 - levulinic acid wild type enzyme, at pH 6.5 and 30°C 737914 1.1.1.30 0.00921 - levulinic acid mutant enzyme H144F, at pH 6.5 and 30°C 737914 1.1.1.30 0.01069 - levulinic acid mutant enzyme H144I, at pH 6.5 and 30°C 737914 1.1.1.30 0.019 - levulinic acid recombinant mutant enzyme W187F with N-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.028 - levulinic acid recombinant mutant enzyme H144L with C-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.02969 - levulinic acid mutant enzyme W187F, at pH 6.5 and 30°C 737914 1.1.1.30 0.06 - levulinic acid recombinant mutant enzyme H144L with N-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.06594 - levulinic acid mutant enzyme H144L, at pH 6.5 and 30°C 737914 1.1.1.30 0.07107 - levulinic acid mutant enzyme H144L/W187L, at pH 6.5 and 30°C 737914 1.1.1.30 0.079 - levulinic acid recombinant mutant enzyme H144L/W187F with C-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.164 - levulinic acid recombinant mutant enzyme H144L/W187F with N-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 0.2565 - levulinic acid mutant enzyme H144L/W187F, at pH 6.5 and 30°C 737914 1.1.1.30 4.5 - acetoacetate mutant T190A 698660 1.1.1.30 4.9 - acetoacetate recombinant enzyme with C-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 5.1 - acetoacetate mutant T190C 698660 1.1.1.30 6.1 - D-3-hydroxybutyrate mutant T190C 698660 1.1.1.30 6.7 - D-3-hydroxybutyrate mutant H6-W257A 698660 1.1.1.30 7.2 - D-3-hydroxybutyrate mutant H6-W187T 698660 1.1.1.30 12 - D-3-hydroxybutyrate mutant H6-W257Y 698660 1.1.1.30 13 - (R)-3-hydroxybutanoate mutant enzyme H141A 679792 1.1.1.30 21 - D-3-hydroxybutyrate mutant H6-H144A 698660 1.1.1.30 22 - D-3-hydroxybutyrate mutant H6-Q196N 698660 1.1.1.30 22 - D-3-hydroxybutyrate mutant T190A 698660 1.1.1.30 29 - D-3-hydroxybutyrate mutant H6-W187A 698660 1.1.1.30 30 - acetoacetate mutant L215A 698660 1.1.1.30 58 - NADH - 695653 1.1.1.30 58 - NADH 30°C 669544 1.1.1.30 70 - D-3-hydroxybutyrate mutant L215A 698660 1.1.1.30 83 - D-3-hydroxybutyrate mutant H6-Q196E 698660 1.1.1.30 87 - acetoacetate mutant L215V 698660 1.1.1.30 87 - NAD+ - 695653 1.1.1.30 87 - NAD+ 30°C 669544 1.1.1.30 88 - D-3-hydroxybutyrate mutant H6-W257F 698660 1.1.1.30 97.16 - acetoacetate recombinant enzyme with N-terminal His-tag, at pH 6.5 and 30°C 737912 1.1.1.30 99 - D-3-hydroxybutyrate mutant H6-Q196A 698660 1.1.1.30 100 - acetoacetate - 695653 1.1.1.30 100 - acetoacetate 30°C 669544 1.1.1.30 110 - acetoacetate - 695653 1.1.1.30 110 - acetoacetate 30°C 669544 1.1.1.30 110 - D-3-hydroxybutyrate mutant H6-K152R 698660 1.1.1.30 120 - acetoacetate wild-type 698660 1.1.1.30 125 - acetoacetate mutant T190S 698660 1.1.1.30 140 - D-3-hydroxybutyrate mutant H6-Q94A 698660 1.1.1.30 150 - acetoacetate - 695653 1.1.1.30 167 - (R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme H141A 668251 1.1.1.30 180 - (R)-3-hydroxybutanoate 30°C 669544 1.1.1.30 180 - D-3-hydroxybutyrate - 695653 1.1.1.30 180 - D-3-hydroxybutyrate mutant H6-W187Y 698660 1.1.1.30 215 - (R)-3-hydroxybutanoate mutant enzyme Q133A 679792 1.1.1.30 280 - NADH - 695653 1.1.1.30 280 - NADH 30°C 669544 1.1.1.30 310 - (R)-3-hydroxybutanoate 30°C 669544 1.1.1.30 310 - D-3-hydroxybutyrate - 695653 1.1.1.30 310 - D-3-hydroxybutyrate H6-HBDH 698660 1.1.1.30 310 - D-3-hydroxybutyrate mutant H6-W187F 698660 1.1.1.30 333 - (R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q193A 668251 1.1.1.30 340 - NAD+ - 695653 1.1.1.30 340 - NAD+ 30°C 669544 1.1.1.30 360 - NAD+ - 695653 1.1.1.30 370 - D-3-hydroxybutyrate wild-type 698660 1.1.1.30 404 - (R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme K149R 668251 1.1.1.30 411 - (R)-3-hydroxybutanoate mutant enzyme Q91A 679792 1.1.1.30 432 - (R)-3-hydroxybutanoate wild type enzyme 679792 1.1.1.30 435 - D-3-hydroxybutyrate mutant L215V 698660 1.1.1.30 500 - D-3-hydroxybutyrate - 695653 1.1.1.30 705 - D-3-hydroxybutyrate mutant T190S 698660 1.1.1.30 740 - NADH - 695653 1.1.1.30 1382 - (R)-3-hydroxybutanoate 23°C, pH 8.0, wild-type enzyme 668251 1.1.1.30 1390 - (R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q91A 668251