6.3.2.8 10 70 purified recombinant His-tagged enzyme, stable up to 40°C, MurC activity diminishes at 45-70°C 728598 6.3.2.8 37 - 28% loss of activity with previous remove of dithiothreitol 651239 6.3.2.8 50 - 5 mM potassium phosphate, pH 7.2, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer 1280