5.4.2.11 additional information - ammonium sulfate or 2,3-diphosphoglycerate stabilizes against elevated temperatures 3258 5.4.2.11 30 - - 3253 5.4.2.11 30 - 30 min, 38% loss of activity in absence of cofactor 3253 5.4.2.11 45 - wild-type, 1 h, 10% loss of activity, mutant M230I, 20 min, 50% loss of activity, 1 h, 90% loss of activity. In presence of 2,3-bisphosphoglycerate, both forms remain stable 661261 5.4.2.11 55 60 neutral pH, presence of ammonium sulfate or 2,3-diphosphoglycerate, stable for several min 3214 5.4.2.11 60 - 30 min, 13% loss of activity in presence of cofactor 3253 5.4.2.11 60 - 30 min, 17% loss of activity in presence of cofactor 3253 5.4.2.11 60 - MM-type isozyme very stable, BB-type and MB-type are inactivated rapidly, 2,3-diphosphoglycerate protects 3248