2.3.1.46 additional information - recombinant enzyme shows high transition temperature of 67.68°C 701784 2.3.1.46 additional information - the transition temperature of wild-type enzyme is 47.075°C, of the mutant strain 333 (S61T/E213V/I229T/N267D/N271K) 48.76°C, of mutant N267D 47.36°C, and of mutant I229T 46.99°C 701784 2.3.1.46 44 - complete aggregation of wild-tyype enzyyme at 44°C 737132