2.1.4.1	additional information	-	inactive CyrA lacks a well-defined tertiary structure at any temperature	719453	
2.1.4.1	additional information	-	the three mutant variants have lower Tmax values than the wild-type with 54°C compared to 48°C, 51.5°C and 49.5°C for F245N, S247M, and F245N/S247M, respectively	719473	
2.1.4.1	44.5	-	an inactivated form of this enzyme retains an appreciable amount of secondary structure elements even on heating to 94°C, but loses its tertiary structure at low temperature of 44.5°C resulting in a state reminiscent of a molten globule. The presence of 500 mm NaCl decreases Tmax at pH 7.5 from 54°C to 49°C	719453	
2.1.4.1	100	-	inactivation by incubation for 5 min	485960