3.4.19.3	additional information	-	alpha6-helix (from Ser188 to Glu205) of PCP-0SH is stably formed in the D1 state	678264	
3.4.19.3	additional information	-	the heat denaturation of wild-type enzyme and mutant enzymes C188S and C142S/C188S is highly reversible in the dimeric forms, but completely irreversible in the tetrameric form	651099	
3.4.19.3	additional information	-	the thermal stability of the mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V, C142S/C188S/E192D and C142S/C188S/E192Q at pH 2.15, pH 3.04 and pH 7.3 is less than that of the cysteine-free mutant enzyme C142S/C188S. At pH 8.7 and 9.6 the thermal stability of mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V and C142S/C188S/E192Q is higher than that of the cysteine-free mutant C142S/C188S. The thermal stability of mutant enzyme C142S/C188S/E192D at pH 8.7 and 9.6 is less than that of cysteine-free mutant enzyme C142S/C188S	667733	
3.4.19.3	7.5	8.5	stable	667525	
3.4.19.3	22	-	room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month	95294	
3.4.19.3	24	-	50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month	95303	
3.4.19.3	30	-	pH 7.0, 30 min, about 5% loss of activity of wild-type enzyme and of mutant enzyme S185C	650352	
3.4.19.3	37	-	1 h, rapid inactivation	95295	
3.4.19.3	40	-	4.5 h, stable up to	653759	
3.4.19.3	40	-	pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable	95290