2.7.2.7 44 60 at 44°C between pH 7.0 and 8.0, the enzyme remains stable and produces linear results up to 30 min under standard assay conditions. When subjected to a 15 min incubation at 60°C, the enzyme retains nearly 70% activity with 2.5 mM ATP and 30% activity with 50 mM butyrate 761102 2.7.2.7 50 - 20 min, 70% loss of activity in absence of 100 mM butanoate, complete protection against inactivation in presence of mM potassium butanoate 642344 2.7.2.7 60 - 1 min, complete loss of activity in absence of butanoate 642344