1.1.1.3 37 - half-life of mutant L200F/D215K is 4.16 h, of mutant L200F 3.71 h 741465 1.1.1.3 50 100 purified recombinant enzyme, 10 min, completely stable at 50-90°C, loss of 40% activity at 100°C 760736 1.1.1.3 54.8 - melting temperature of recombinant enzyme BsHSD 761687 1.1.1.3 70 - up to 246393 1.1.1.3 75 - purified recombinant His-tagged enzyme, 10 min, retains full activity 741408 1.1.1.3 80 - slow inactivation, protection by K+, Na+ 246393 1.1.1.3 82 - Tm of wild-type TtHSD 761404 1.1.1.3 90 - purified recombinant His-tagged enzyme, 10 min, retains 70% activity 741408 1.1.1.3 90 - rapid inactivation 246393