5.4.99.15 40 - 60 min, stable up to 3486 5.4.99.15 60 75 temperature stability of recombinant SEB24 and SP3.5, both of them have a half-life of 12 h at 75°C. The half-lives of SEB24 and SP3.5 at 60°C are 6 d and 6.5 d, respectively. SP3.5 and SEB24 retain 80% and 97% of their original activity after incubation for 2 days at 60°C 749205 5.4.99.15 70 - 48 h, 80% residual activity 681347 5.4.99.15 70 80 the MTSH fusion enzyme exhibits a high degree of thermostability, retaining 80% of the activity when pre-incubated at 70°C for 48 h, the stability is gradually abolished by incubating the fusion enzyme at above 80°C for 30 min 693601 5.4.99.15 75 - MTS proves stable at 75°C for more than 24 h, with a half life of about 50 h 693473 5.4.99.15 75 85 purified recombinant enzyme, pH 5.0, 10 h, highly stable 748824 5.4.99.15 80 - 2 h, activity remains unchanged 724948 5.4.99.15 80 - 72 h, citrate-phosphate buffer, 38% loss of activity 651359 5.4.99.15 85 - 2 h, 45% loss of activity 724948 5.4.99.15 85 - 6 h, 9% loss of activity 3487, 721858, 722996 5.4.99.15 85 - 60 min, stable up to 3488 5.4.99.15 85 - activity is gradually abolished 681347 5.4.99.15 85 - pH 4.5, 120 min, about 10% loss of activity 727449 5.4.99.15 90 - 1 h, 30% loss of activity 651359 5.4.99.15 95 - 2 h, 90% loss of activity 724948 5.4.99.15 95 - purified recombinant enzyme, pH 5.0, 8 h, 60% activity remaining 748824 5.4.99.15 100 - 1 h, 65% loss of activity 651359