3.4.23.23 additional information - - 30654, 30659, 30668 3.4.23.23 20 50 - 650661 3.4.23.23 37 - at 37°C, the enzyme residual activity is 94 and 68% of the initial one after 120 and 150 min of incubation, respectively 754256 3.4.23.23 38 - pH 3.0-6.0, 8 days, more than 90% of activity retained 30668 3.4.23.23 40 - stable up to, acid protease D 30649 3.4.23.23 40 60 stable up to 40°C, 26% residual proteolytic activity at 50°C, 13% residual proteolytic activity at 60°C 653000 3.4.23.23 45 - stable a up to, for 60 min 743030 3.4.23.23 45 - stable up to, acid protease C 30649 3.4.23.23 50 55 upon 60 min incubation period enzyme activity begins to decrease at a temperature above of 50 °C. After 1 h of incubation at 55°C, 75% of the unpurified enzyme's activity is retained 752456 3.4.23.23 55 - 20% loss of activity after 15 min 30654 3.4.23.23 55 - 90% loss of activity after 10 min 30651 3.4.23.23 55 - the wild type enzyme retains 88.6% activity after 60 min at 55°C. Mutant enzyme G186D/E13D loses 30% of its activity after 50 min at 55°C 754970 3.4.23.23 65 - 15 min, 40% loss of activity 30678