3.4.23.20 25 45 the purified enzyme does not lose any activity over 48 h at 25°C. The protease shows an activity loss of 77.56% at 45°C after 12 h 755308 3.4.23.20 30 - pH 2.5-6.0, 20 h, stable 30595 3.4.23.20 30 - pH 2.5-6.5, 24 h, stable 30586 3.4.23.20 30 50 the enzyme remains stable after 80 min incubation at 30-50°C (pH 5.0). The activity drops to less than 20% after 80 min at 60°C. The enzyme shows about 75% activity after 2 h at 50°C 752532 3.4.23.20 37 - pH 3.0 or pH 6.1, slow inactivation 30585 3.4.23.20 55 - pH 4.9, 1 h, stable 30585 3.4.23.20 60 - pH 3.5-5.5, 1 h, stable 30586, 30595 3.4.23.20 70 - pH 4.5, 1 h, more than 65% of activity is retained 30595 3.4.23.20 80 - pH 4.5, 10 min, complete inactivation 30595