3.4.23.18 40 - below, stable 30560 3.4.23.18 60 - 20 min, at pH 2.8, complete inactivation 30560 3.4.23.18 55 - up to, stable, at pH 3.8 30561 3.4.23.18 50 - up to, stable, at pH 3.8 30563 3.4.23.18 20 25 pH 2.7, 16 h stable 30564 3.4.23.18 30 - pH 7, drastic decrease of activity probably due to autolytic activity 30564 3.4.23.18 55 - 20 min, complete inactivation at pH 5.5, Ca2+ does not protect 30564 3.4.23.18 55 - 10 min, in 50 mM sodium acetate buffer, pH 5 30569, 30570, 30575 3.4.23.18 55 - isozyme A1 loses 10% of its original activity in contrast to almost complete loss of activity of isozyme A2 30569 3.4.23.18 55 - isozyme F1 retains 70%, F2 85% of original activity 30570 3.4.23.18 55 - isozyme M1 retains more than 95% of its original activity, M2 is completely inactivated 30575 3.4.23.18 50 80 the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h 752341 3.4.23.18 30 50 the enzyme maintains 39.8% of its maximum activity after 5 min incubation at 50°C. The protease remains stable after 1 h of incubation at 30 and 35°C, retaining more than 70% activity 753450 3.4.23.18 50 - the enzyme maintains 80% activity when incubated at 50°C and pH 3.0 for 20 min 754494 3.4.23.18 20 45 after incubation at 20°C for 1 h, the enzyme with propeptide retains 100% activity. 38.1°C is the melting temperature of the catalytically active enzyme with propeptide. When the temperature is over 45°C, the relative enzyme activities of the enzyme with propeptide decreases to below 40% 755227