3.1.3.72 additional information - heat stable 3.1.3.72 50 - 10 min, stable 3.1.3.72 55 - 40 min: no loss of activity, 80 min: 50% loss of activity 3.1.3.72 55 - 10 min, 60% loss of activity in assay containing CaCl2, compared to 40% in absence of CaCl2 3.1.3.72 60 - ca. 80% activity at pH 8.0 for up to 3 h in the presence/absence of 5 mM CaCl2. In the presence/absence of 5 mM CaCl2, phytase is also stable at 80°C and shows ca. 100% of residual activity for 30 min at pH 8.0 and then declines sharply thereafter