2.7.4.8 additional information - thermostability of the enzyme is found to be significantly enhanced in the presence of the substrates 761281 2.7.4.8 30 - at least 15 min stable from pH 5.5-8.5 642688 2.7.4.8 42.4 - Tm value, mutant enzyme R96H 761496 2.7.4.8 43.8 - Tm value, mutant enzyme S121F 761496 2.7.4.8 44.4 - Tm value, mutant enzyme V91M 761496 2.7.4.8 44.6 - Tm value, mutant enzyme S186Y 761496 2.7.4.8 45 - t1/2: 10 min 642690 2.7.4.8 45.5 - Tm value, mutant enzyme S2L 761496 2.7.4.8 45.6 - Tm value, mutant enzyme R116Q 761496 2.7.4.8 46 - Tm value, mutant enzyme G3A 761496 2.7.4.8 47.4 - Tm value, wild-type enzyme 761496 2.7.4.8 50 - 10 min, inactivation 642688 2.7.4.8 60 - 10 min, 90% loss of activity 642690 2.7.4.8 80 - incubation for 2 min, 5 min, 10 min or 20 min leads to 43%, 67%, 89% or 97% loss of activity, respectively, 30 min: inactivation 642684 2.7.4.8 100 - 10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation) 642685