2.7.4.25 additional information - thermal stability of CMP kinase genetic variants, overview 692282 2.7.4.25 43 - mutant D132A 676227 2.7.4.25 45 - mutant R110M 676227 2.7.4.25 47 - mutant D132N 676227 2.7.4.25 48 - midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP 645159 2.7.4.25 48 - mutant D132S, mutant D132H 676227 2.7.4.25 48.5 57.8 the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5°C, the melting temperature of CMP kinae in the presence of ATP is at 57.8°C, the melting temperature of CMP kinae in the presence of CMP is at 50.5°C 645159 2.7.4.25 49 - midpoint denaturation temperature in absence of nucleotide substrates 645159 2.7.4.25 49 - midpoint denaturation temperature in presence of CMP 645159 2.7.4.25 51 - midpoint denaturation temperature in presence of CMP 645159 2.7.4.25 51 - mutant R188M 676227 2.7.4.25 52 - CMP kinase is half-inactivated at 52°C 645154 2.7.4.25 52 - the melting temperature of the wild type enzyme is at 52°C 712736 2.7.4.25 52 - wild type, mutant S36A 676227 2.7.4.25 58 - midpoint denaturation temperature in presence of ATP 645159