3.4.22.44 15 - temperature optimum for the 24 kDa protein 137276 3.4.22.44 15 - temperature optimum of the 27 kDa full length protease. The optimum is not related to the C-terminal cleavage site but to the stability or flexibility of the structure of the NIa protease 137277 3.4.22.44 25 - assay at 718264, 718449 3.4.22.44 30 - assay at 718366