3.4.23.23 30 - for milk-clotting activity 653000 3.4.23.23 35 - for proteolytic activity 653000 3.4.23.23 37 - - 754256 3.4.23.23 40 - acid protease D 30649 3.4.23.23 45 - - 717133 3.4.23.23 45 - acid protease C 30649 3.4.23.23 49 55 Mucor pusillus 30652 3.4.23.23 50 - - 30654, 717133 3.4.23.23 55 - - 30679, 717133, 743030 3.4.23.23 58 62 - 717133 3.4.23.23 60 - - 717133, 752456 3.4.23.23 60 - purified mutant G186D/E13D and non-mutant enzymes act optimally at 60°C and later, they start losing their activity rapidly leading to complete inactivation at 75°C 754970 3.4.23.23 63 - above, Mucor miehei 30652