3.4.23.23 60 - - 717133, 752456 3.4.23.23 60 - purified mutant G186D/E13D and non-mutant enzymes act optimally at 60°C and later, they start losing their activity rapidly leading to complete inactivation at 75°C 754970 3.4.23.23 63 - above, Mucor miehei 30652