7.4.2.6 - 7.4.2.6 cloning and gene organization of the bldK locus, overview 7.4.2.6 cloning in Escherichia coli JM109, PepT1 translation in Xenopus oocytes 7.4.2.6 DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression using the pAN701 plasmid 7.4.2.6 expressed in Escherichia coli BL21(DE3) cells 7.4.2.6 expressed in Lactococcus lactis strain NZ9000 7.4.2.6 expressed in lung, liver and spleen of mice 7.4.2.6 expression of Arabidopsis thaliana AtOPT6 in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae 7.4.2.6 expression of C-terminal His6-tagged OppA, OppA I602C, and OppBCDF His6-tag on C-terminus of OppC in Lactococcus lactis 7.4.2.6 four opp operons, opp-1, opp-2, opp-3, and opp-4, plus an opp-5A gene encoding a putative peptide-binding protein, DNA and amino acid sequence determination and analysis, opp-1, opp-2, opp-3, and opp-4 operons are polycistronic and opp-5A is monocistronic, transcriptional profiles and sequence comparison. Gene opp-3, which encodes proteins most similar to known peptide transport proteins, displays the highest expression level, overview