2.7.7.47	27.8 kb R-plasmid pTET3 gene cassette aadA9, antibiotic resistance region identified in Mycobacterium fortuitum, cloned in Escherichia coli DH5alphaMCR	
2.7.7.47	aadA resistence gene transferred vi filter mating from Enterococcus faecalis W4770 to Enterococcus faecalis JH2-2, PCR amplified aadA gene cloned into vector pCRII and transformed into Escherichia coli DH5-alpha	
2.7.7.47	cloned in Escherichia coli BL21	
2.7.7.47	expressed in Escherichia coli DH5alpha cells	
2.7.7.47	expression in Escherichia coli	
2.7.7.47	gene aadA2B isolated from integron InC as a gene cassette, Escherichia coli cells transformed by electroporation	
2.7.7.47	into the vector pGEM-T for sequencing	
2.7.7.47	streptomycin adenylyltransferase gene isolated from unconjugative plasmids of Enterococcus casseliflavus HZ95 is cloned in Escherichia coli DH5alpha	
2.7.7.47	the plastid expression vector pPRV111A carrying the aadA gene encoding aminoglycoside 3''-adenylyltransferase is used for plastid transformation in Solanum melongena	
2.7.7.47	thioredoxin-His6-tagged SMATase fusion protein is produced in a bacterial intracellular expression system mainly in a soluble form