2.3.1.33 gene hisat, DNA and amino acid sequence determination and analysis 735700 2.3.1.33 gene hisat, DNA and amino acid sequence determination and analysis, the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens, recombinant expression in a cell-free translation system, and recombinant expression of the two HISAT splice variant-1 and -2 in Oreochromis niloticus 735700 2.3.1.33 gene Nat16, DNA and amino acid sequence determination and analysis, recombinant expression in a cell-free translation system 735700