2.1.2.11 expressed in Escherichia coli strain BW25113 or MG1655 756787 2.1.2.11 expressed in Escherichia coli W3110 cells 756758 2.1.2.11 gene panB, phylogenetic analysis, genes panBC in Rhizobiales with multipartite genomes, overview 719177 2.1.2.11 panB gene coding for enzyme is located together with panE in the intervall purE-tre of the chromosome 485705 2.1.2.11 panB gene encoding enzyme is cloned and overexpressed in Escherichia coli BL21 (DE3) 485710 2.1.2.11 panB gene encoding enzyme is cloned, sequenced and 50fold overexpressed in Escherichia coli panB mutant Hfr3000 YA139 containing plasmid pCEJ01, gene is 792 bp long and encodes a protein of 264 amino acids, gene is likely to be cotranscribed with at least one other gene, panB, panC and panD genes are closely clustered at 3.1 min of the Escherichia coli K12 genetic map 485707 2.1.2.11 panB gene encoding enzyme is cloned, sequenced and expressed in Escherichia coli as an active octameric enzyme, ORF encodes a protein of 349 amino acids, panB gene is closely linked to nimO gene on Aspergillus nidulans linkage group VII 485708 2.1.2.11 panB gene encoding enzyme is cloned, sequenced and overexpressed in Escherichia coli BL21, ORF of 846 bp encodes a protein of 281 amino acids, N-terminal 37 amino acids are essential for enzyme function 485706 2.1.2.11 panB gene encoding enzyme is clustered together with other pantothenate biosynthetic genes panC and panD at 3 min of the chromosome map, panBCD operon 485709 2.1.2.11 PCR amplification of panB gene, which is identical to that of Mycobacterium bovis BCG 485706