1.7.2.3	-	
1.7.2.3	amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein	
1.7.2.3	expressed in Escherichia coli	
1.7.2.3	expression in strain BL21(DE3) as His-tagged protein	
1.7.2.3	fusion protein with beta-galactosidase	
1.7.2.3	gene torA, the torCAD operon encodes the Tor system induced in the presence of trimethylamine oxide mainly during exponential phase	
1.7.2.3	gene torD, DNA sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as His-tagged protein	
1.7.2.3	GFP-tagged enzyme expression, the genome sequence of Vibrio fischeri reveals three putative TMAO reductase operons, transcriptional regulation, overview	
1.7.2.3	overexpression	
1.7.2.3	overexpression as His-tagged protein